动物营养学报 >

2019 , Vol. 31 >Issue 1: 274 - 284

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.034

实验方法 Experimental Methods

体外产气法研究植物精油对肉羊体外瘤胃发酵参数及甲烷产量的影响

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  • 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206
石宁(1987-),男,河北石家庄人,硕士研究生,研究方向为反刍动物营养与饲料科学。E-mail:522583272@qq.com

收稿日期: 2018-06-13

  网络出版日期: 2019-01-16

基金资助

国家自然科学基金项目（31302000）；北京市农业科技项目（20170131）

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Effects of Plant Essential Oil on Rumen Fermentation Parameters and Methane Production of Mutton Sheep in Vitro

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  • Animal Science and Technology College, Beijing University of Agricultural, Beijing Key Laboratory of Dairy Cow Nutrition, Beijing 102206, China

Received date: 2018-06-13

  Online published: 2019-01-16

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摘要

本试验旨在采用体外产气法研究4种天然植物精油（桉叶油、山苍子油、肉桂油和茴香油）对肉羊体外瘤胃发酵参数及甲烷（CH4）产量的影响。体外培养底物精粗比为60：40，分别添加0（对照）、50、100、200和400 mg/L的桉叶油、山苍子油、肉桂油和茴香油，每种植物精油的每个浓度设置3个重复，体外模拟瘤胃发酵培养24 h，测定24 h产气量和气体中的CH4产量以及瘤胃发酵液的pH、挥发性脂肪酸（VFA）和氨态氮（NH3-N）浓度。结果表明：1）添加山苍子油、茴香油显著影响了体外瘤胃发酵液pH（P<0.05）。2）与对照组相比，添加400 mg/L山苍子油、茴香油显著降低了总VFA浓度（P<0.05），且随着山苍子油、茴香油添加浓度的增加呈线性下降趋势（P<0.05）；添加400 mg/L山苍子油和茴香油均显著增加了乙酸比例（P<0.05），且随着山苍子油和茴香油添加浓度的增加呈先降低后升高的二次曲线趋势（P<0.05）；添加200 mg/L茴香油、400 mg/L桉叶油显著提高了丙酸比例（P<0.05），而添加400 mg/L的山苍子油显著降低了丙酸比例（P<0.05），且随着桉叶油、山苍子油、茴香油添加浓度的增加呈二次曲线变化趋势（P<0.05）；添加400 mg/L桉叶油、200 mg/L茴香油显著降低了乙酸/丙酸（P<0.05），添加400 mg/L山苍子油显著增加了乙酸/丙酸（P<0.05），且随着桉叶油、山苍子油、茴香油添加浓度的增加呈二次曲线变化趋势（P<0.05）。3）与对照组相比，添加400 mg/L茴香油显著降低了NH3-N含量（P<0.05），且随着茴香油添加浓度的增加呈线性下降趋势（P<0.05）。4）与对照组相比，添加400 mg/L山苍子油、茴香油均显著降低了产气量（P<0.05），且随则山苍子油添加浓度的增加呈先增加后降低的二次曲线趋势（P<0.05），随着茴香油添加浓度的增加呈线性下降趋势（P<0.05）；添加400 mg/L山苍子油、茴香油显著降低了CH4产量（P<0.05），且随着山苍子油添加浓度的增加呈先增加后降低的二次曲线趋势（P<0.05），随着茴香油添加浓度的增加呈线性下降趋势（P<0.05）。由此可见，不同植物精油对体外瘤胃发酵参数和CH4产量的影响结果不同，且与植物精油浓度有关。

关键词： 植物精油; 瘤胃发酵参数; 甲烷产量

本文引用格式

石宁, 贾淼, 李艳玲 . 体外产气法研究植物精油对肉羊体外瘤胃发酵参数及甲烷产量的影响[J]. 动物营养学报, 2019 , 31(1) : 274 -284 . DOI: 10.3969/j.issn.1006-267x.2019.01.034

Abstract

The objective of this experiment was to study the effects of four kinds of plant essential oils (eucalyptus oil, litsea cubeba oil, cinnamon oil and anise oil)on rumen fermentation parameters and methane (CH4) production of mutton sheep in vitro. The ratio of concentrate to forage of the cultivation substrates was 60:40, and 0 (control), 50, 100, 200 and 400 mg/L of eucalyptus oil, litsea cubeba oil, cinnamon oil or anise oil were added, respectively. There were 3 replicates for each concentration of four plant essential oils. The 24 h gas production, CH4 proportion in gas, pH, volatile fatty acid (VFA) and ammonia nitrogen (NH3-N) concentrations were measured for 24 h simulating rumen fermentation cultivation in vitro. The results showed as follows:1) adding litsea cubeba oil and anise oil significantly affected the rumen fermentation fluid pH in vitro (P<0.05). 2) Compared with the control group, adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the total VFA concentration (P<0.05), and showed a linearly decreased trend with the concentrations of litsea cubeba oil and anise oil increased (P<0.05); adding 400 mg/L litsea cubeba oil and anise oil significantly increased the acetic acid proportion (P<0.05), and showed a firstly decreased and then increased quadratically trend with the concentration of litsea cubeba oil and anise oil increased (P<0.05); adding 200 mg/L anise oil and 400 mg/L eucalyptus oil significantly increased the propionic acid proportion (P<0.05), adding 400 mg/L litsea cubeba oil significantly decreased the propionic acid proportion (P<0.05), and showed a quadratic curve change trend with the concentrations of eucalyptus oil, litsea cubeba oil and anise oil increased (P<0.05); adding 400 mg/L eucalyptus oil and 200 mg/L anise oil significantly decreased the ratio of acetic acid to propionic acid (P<0.05), adding 400 mg/L litsea cubeba oil significantly increased the ratio of acetic acid to propionic acid (P<0.05), and showed a quadratic curve change trend with the concentrations of eucalyptus oil, anise oil and litsea cubeba oil increased (P<0.05). 3) Compared with the control group, adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the NH3-N concentration (P<0.05), and showed a linearly decreased trend with the concentration of anise oil increased (P<0.05). 4) Compared with the control group, adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the gas production (P<0.05), and showed a firstly increased and then decreased quadratically trend with the concentration of litsea cubeba oil increased (P<0.05), and showed a linearly decreased trend with the concentration of anise oil increased (P<0.05); adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the CH4 production (P<0.05), and showed a firstly increased and then decreased quadratically trend with the with the concentration increase of litsea cubeba oil increased (P<0.05), and showed a linearly decreased trend with the concentration of anise oil increased. (P<0.05). The results indicate that different plant essential oils have different effects on rumen fermentation parameters and CH4 production in vitro, and which are related to the concentration of essential oils.

Key words： plant essential oil; rumen fermentation parameters; methane production

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