沙葱及其提取物对小尾寒羊生长性能和脂肪代谢相关指标的影响

张秀媛, 李书仪, 王翠芳, 丁赫, 敖长金

doi:10.3969/j.issn.1006-267x.2019.01.040

动物营养学报 >

2019 , Vol. 31 >Issue 1: 334 - 341

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.040

饲料科学与技术 Feed science and technology

沙葱及其提取物对小尾寒羊生长性能和脂肪代谢相关指标的影响

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1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 河北北方学院农林科技学院, 张家口 075000

张秀媛(1982-),女,山西平遥人,博士研究生,研究方向为动物营养与畜产品品质。E-mail:zhangxiuyuan917@163.com

收稿日期: 2018-06-29

网络出版日期: 2019-01-16

基金资助

国家自然科学基金项目（31460611）

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Effects of Allium mongolicum Regel and Its Extract on Growth Performance and Lipid Metabolism Related Indices of Small Tail Han Sheep

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1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. School of Agriculture and Forestry Science and Technology, Hebei North College, Zhangjiakou 075000, China

Received date: 2018-06-29

Online published: 2019-01-16

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摘要

本试验旨在研究沙葱及其提取物对小尾寒羊生长性能和脂肪代谢相关指标的影响。选用月龄相近、体重35 kg左右的健康小尾寒羊40只，随机分为4组，每组10只羊。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加0.1%沙葱多糖（多糖组）、10 g/（d·只）沙葱粉（沙葱组）、10 g/（d·只）沙葱提取多糖后的滤渣（滤渣组）。预试期15 d，正试期60 d。结果表明：1）与对照组相比，滤渣组的平均日采食量（ADFI）显著增加（P<0.05），多糖组和沙葱组的ADFI均有增加趋势（0.05 ≤ P<0.10）；沙葱组和滤渣组的平均日增重（ADG）均有增加趋势（0.05 ≤ P<0.10）；3个试验组的料重比（F/G）均无显著差异（P>0.05）。2）与对照组相比，3个试验组的背最长肌中肌内脂肪（IMF）含量均无显著差异（P>0.05）。3）与对照组相比，3个试验组的血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）含量均无显著差异（P>0.05），3个试验组的血清瘦素（LEP）含量显著升高（P<0.05）。4）与对照组相比，多糖组和滤渣组背最长肌中二酰甘油酰基转移酶1（DGAT1）mRNA表达量显著降低（P<0.05），沙葱组和滤渣组背最长肌中乙酰辅酶A羧化酶（ACC）、硬脂酰辅酶A去饱和酶（SCD）mRNA表达量均显著降低（P<0.05），多糖组背最长肌中激素敏感脂肪酶（HSL）mRNA表达量显著升高（P<0.05）。由此可见，沙葱、沙葱多糖、沙葱提取多糖后的滤渣均不能显著改善小尾寒羊生长性能和背最长肌中肌内脂肪沉积，但均可显著提高血清LEP含量，并且可以不同程度地调节背最长肌中脂肪代谢相关基因的mRNA表达量。

关键词： 沙葱; 沙葱多糖; 小尾寒羊; 生长性能; 脂肪代谢; 基因

本文引用格式

张秀媛, 李书仪, 王翠芳, 丁赫, 敖长金 . 沙葱及其提取物对小尾寒羊生长性能和脂肪代谢相关指标的影响[J]. 动物营养学报, 2019 , 31(1) : 334 -341 . DOI: 10.3969/j.issn.1006-267x.2019.01.040

Abstract

This experiment was conducted to investigate effect of Allium mongolicum Regel (AMR) and its extract on growth performance and lipid metabolism related indices of small tail Han sheep. Forty healthy small tail Han sheep with the similar age and body weight (about 35 kg) were randomly divided into 4 groups with 10 sheep per group. Sheep in the control group were fed a basal diet, sheep in the experimental groups were fed the basal diet supplemented with 0.1% AMR polysaccharide (polysaccharide group), 10 g/(d·sheep) AMR meal (AMR group), 10 g/(d·sheep) filter residue from AMR extracted polysaccharide (filter residue group), respectively. The pre-experimental period lasted for 15 days, the formal experiment period lasted for 60 days. The results showed as follows:1) compared with the control group, the average daily feed intake (ADFI) of filter residue group was significantly increased (P<0.05), the ADFI of polysaccharide group and AMR group showed an increasing trend (0.05 ≤ P<0.10); the average daily gain (ADG) of AMR group and filter residue group showed an increasing trend (0.05 ≤ P<0.10), the feed to gain ratio (F/G) of three experimental groups was no significant difference (P>0.05). 2) Compared with the control group, the content of intramuscular fat (IMF) in longissimus dorsi of three experimental groups was no significant difference (P>0.05). 3) Compared with the control group, the contents of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in serum of three experimental groups were no significant difference (P>0.05), and the serum leptin (LEP) content of three experimental groups was significantly increased (P<0.05). 4) Compared with the control group, the diacylglycerol acyltransferase 1 (DGAT1) mRNA expression of polysaccharide group and filter residue group was significantly decreased (P<0.05), the acetyl-CoA carboxylase (ACC) and stearoyl-CoA desaturase (SCD) mRNA expressions of AMR group and filter residue group were significantly decreased (P<0.05), the hormone-sensitive lipase (HSL) mRNA expression of polysaccharide group was significantly increased (P<0.05). It is concluded that AMR, AMR polysaccharide and filter residue from AMR extracted polysaccharide cannot significantly improve the growth performance and the deposition of IMF in longissimus dorsi of small tail Han sheep, but it can significant improve the serum LEP content, and can differently regulate the mRNA expression of lipid metabolism related genes.

Key words： Allium mongolicum Regel; AMR polysaccharide; small tail Han sheep; growth performance; lipid metabolism; gene

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