动物营养学报 >

2019 , Vol. 31 >Issue 1: 342 - 350

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.041

饲料科学与技术 Feed science and technology

不同浓度乙醇洗脱沙葱黄酮对脂多糖诱导的小鼠腹腔巨噬细胞的抗炎作用

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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
王翠芳(1990-),女,内蒙古赤峰人,博士研究生,从事动物营养与饲料研究。E-mail:cuifangwang2017@163.com

收稿日期: 2018-06-25

  网络出版日期: 2019-01-16

基金资助

国家自然科学基金项目（31260558）

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Anti-Inflammatory Effects of Different Concentrations of Ethanol Elutions of Allium mongolicum Regel Flavonoids on Lipopolysaccharide-Induced Mouse Peritoneal Macrophage

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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-06-25

  Online published: 2019-01-16

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摘要

本试验旨在研究通过超声波法提取的35%和75%乙醇洗脱沙葱黄酮对脂多糖（LPS）诱导的小鼠腹腔巨噬细胞的抗炎作用。采用细胞计数试剂盒检测不同添加浓度（25、50、100、200、400 μg/mL）的35%或75%乙醇洗脱沙葱黄酮对小鼠腹腔巨噬细胞活性的影响；设空白对照组、LPS应激模型组、不同浓度乙醇洗脱沙葱黄酮组（分别加入25、50、100 μg/mL 35%或75%乙醇洗脱沙葱黄酮溶液），采用Griess、实时荧光定量PCR及酶联免疫吸附测定法检测不同浓度乙醇洗脱沙葱黄酮对小鼠腹腔巨噬细胞细胞因子及炎性因子的影响。结果表明：与空白对照组相比，35%、75%乙醇洗脱沙葱黄酮在25~100 μg/mL添加浓度内可极显著提高小鼠腹腔巨噬细胞活性（P<0.01）。与LPS应激模型组相比，35%、75%乙醇洗脱沙葱黄酮均可极显著极显著降低小鼠腹腔巨噬细胞一氧化氮含量及诱导型一氧化氮合酶mRNA相对表达量（P<0.01），并均呈剂量依赖效应，且75%乙醇洗脱沙葱黄酮作用效果优于35%乙醇洗脱沙葱黄酮；35%乙醇洗脱沙葱黄酮可极显著降低小鼠腹腔巨噬细胞肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）含量（P<0.01），极显著降低TNF-α、IL-6 mRNA相对表达量（P<0.01）；75%乙醇洗脱沙葱黄酮可显著或极显著降低小鼠腹腔巨噬细胞TNF-α含量（P<0.05或P<0.01），极显著降低TNF-α、IL-6 mRNA相对表达量（P<0.01）。由此可见，35%、75%乙醇洗脱沙葱黄酮可通过提高小鼠腹腔巨噬细胞活性、调控细胞因子及炎性因子的分泌而发挥抗炎作用。

关键词： 沙葱; 黄酮; 脂多糖; 小鼠; 腹腔巨噬细胞; 炎症

本文引用格式

王翠芳, 王特日格乐, 丹妮, 杜红喜, 张秀媛, 萨茹丽, 敖长金 . 不同浓度乙醇洗脱沙葱黄酮对脂多糖诱导的小鼠腹腔巨噬细胞的抗炎作用[J]. 动物营养学报, 2019 , 31(1) : 342 -350 . DOI: 10.3969/j.issn.1006-267x.2019.01.041

Abstract

This experiment was conducted to explore the anti-inflammatory effects of 35% and 75% ethanol elutions of Allium mongolicum Regel flavonoids (AMF) on lipopolysaccharide (LPS)-induced mouse peritoneal macrophage (MPM). The effects of different concentrations (25, 50, 100, 200 and 400 μg/mL) of 35% and 75% ethanol elutions of AMF on cell viability of MPM were examined by cell counting kit-8 assay. The blank control group, LPS stress model group, different concentration ethanol elutions of AMF groups (supplemented with 25, 50 and 100 μg/mL 35% or 75% ethanol elutions of AMF, respectively) were set up, and the effects different concentrations of ethanol elutions of AMF on cytokines and inflammatory factors of MPM were determined using Griess, real time PCR and enzyme-linked immune sorbent assay methods. The result showed that compared with the blank control group, supplemented with 25 to 100 μg/mL 35% and 75% ethanol elutions of AMF could significantly increase the cell viability of MPM (P<0.01). Compared with the LPS stress model group, 35% and 75% ethanol elutions of AMF could significantly decrease the nitric oxide content and inducible nitric oxide synthase mRNA relative expression in MPM (P<0.01), and showed a dose-dependent effect, and the effect of 75% ethanol elutions of AMF was better than 35% ethanol elutions of AMF; 35% ethanol elutions of AMF could significantly decrease the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in MPM (P<0.01), and also could significantly decrease the TNF-α and IL-6 mRNA relative expression (P<0.01); 75% ethanol elutions of AMF could significantly decrease the contents of TNF-α in MPM (P<0.05 or P<0.01), and also could significantly decrease the TNF-α and IL-6 mRNA relative expression (P<0.01). In conclusion, 35% and 75% ethanol elutions of AMF can exert its anti-inflammatory effect via improving cell viability of MPM and affecting secretion of cytokines and inflammatory factors.

Key words： Allium mongolicum Regel; flavonoids; LPS; mouse; peritoneal macrophage; inflammation

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