动物营养学报 >

2019 , Vol. 31 >Issue 1: 477 - 484

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.056

研究简报 Short communications

不同长度的饲用苎麻青贮对黑山羊瘤胃发酵参数及纤维素酶活性的影响

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  • 1. 中国农业科学院麻类研究所, 长沙 410205;
    2. 扬州大学动物科学与技术学院, 扬州 225009;
    3. 中国科学院东北地理与农业生态研究所, 长春 130102
孙盛楠(1987-),女,内蒙古呼伦贝尔人,讲师,博士,从事牧草生理生态学研究。E-mail:snsun@yzu.edu.cn

收稿日期: 2018-06-13

  网络出版日期: 2019-01-16

基金资助

国家自然科学基金项目（31501988）；湖南省重点研发计划（2016NK2170）

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Effects of Different Lengths of Feeding-Ramie Silage on Rumen Fermentation Parameters and Cellulase Activities of Black Goats

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  • 1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;
    2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    3. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China

Received date: 2018-06-13

  Online published: 2019-01-16

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摘要

本试验旨在研究不同长度的饲用苎麻青贮对黑山羊瘤胃发酵参数及纤维素酶活性的影响。选择健康、年龄相近、体重为（20.35±2.16）kg的黑山羊12只，随机分为3组（每组4只），分别饲喂由不同长度（1、2和3 cm）的饲用苎麻青贮为粗饲料配制的全混合日粮（TMR）。在饲喂第11天时，分别于晨饲后0.5、2.0和6.0 h，经口腔采集瘤胃内容物，进行瘤胃发酵参数及纤维素酶活性测定。结果表明：1）饲用苎麻青贮长度对平均日采食量无显著影响（P>0.05）；在晨饲后0.5和2.0 h，1 cm组的瘤胃液pH显著低于2和3 cm组（P<0.05）；在晨饲后2.0 h，1 cm组的瘤胃液氨态氮浓度最低，且显著低于2和3 cm组（P<0.05）。2）饲用苎麻青贮长度对瘤胃液乙丙比有显著影响（P=0.026），3 cm组在各个时间点均低于1和2 cm组，但是对单一挥发性脂肪酸及总挥发性脂肪酸浓度均无显著影响（P>0.05）。3）饲用苎麻青贮长度对瘤胃液各纤维素酶活性均无显著影响（P>0.05），采样时间对瘤胃液β-葡萄糖苷酸酶、果胶酶及木聚糖酶活性均有显著影响（P<0.05），饲用苎麻青贮长度与采样时间对瘤胃液羧甲基纤维素酶及木聚糖酶活性有显著交互作用（P<0.05）。由此可见，在本试验条件下，长度为3 cm的饲用苎麻青贮更有利于黑山羊瘤胃发酵。

关键词： 饲用苎麻; 青贮; 瘤胃发酵; 纤维素酶活性

本文引用格式

孙盛楠, 林谦, 向海, 侯振平, 钟荣珍, 戴求仲, 吴端钦 . 不同长度的饲用苎麻青贮对黑山羊瘤胃发酵参数及纤维素酶活性的影响[J]. 动物营养学报, 2019 , 31(1) : 477 -484 . DOI: 10.3969/j.issn.1006-267x.2019.01.056

Abstract

This experiment was conducted to investigate the effects of different lengths of feeding-ramie silage on rumen fermentation parameters and cellulase activities of black goats. Twelve black goats with similar body weight[(20.35±2.16) kg] were randomly divided into 4 groups with 4 goats per group. Goats in the 4 groups were fed total mixed rations (TMRs) which used the feeding-ramie silage as a roughage, and the lengths of feeding-ramie silage were 1, 2 and 3 cm, respectively. On the 11th day of feeding, the rumen contents were collected by the oral cavity at 0.5, 2.0 and 6.0 h after morning feeding, and the rumen fermentation parameters and cellulase activities were measured. The results showed as follows:1) the length of feeding-ramie silage had no significant effect on average daily intake of goats (P>0.05). The pH in rumen fluid of 1 cm group was significantly lower than that of 2 and 3 cm groups at 0.5 and 2.0 h after morning feeding (P<0.05). The rumen fluid ammonia nitrogen concentration of 1 cm group was the lowest, and it significantly lower than that of 2 and 3 cm groups at 2.0 h after morning feeding (P<0.05). 2) The length of feeding-ramie silage had significant effects on the acetic acid to propionic acid ratio (P=0.026), especially the acetic acid to propionic acid ratio of 3 cm group was lower than that of other two groups at all time points, but there were no significant effects on individual volatile fatty acids (VFAs) and total VFAs (P>0.05). 3) The length of feeding-ramie silage had no significant influences on cellulose activities in rumen fluid (P>0.05), but the sampling time had a significant effect on β-glucuronidase, pectinase and xylanase activities in rumen fluid (P<0.05), and the feeding-ramie silage length and sampling time had significant interaction effects on carboxymethyl cellulase and xylanase activities in rumen fluid (P<0.05). In conclusion, the feeding-ramie silage with 3 cm chopped length is more conducive to rumen fermentation of black goats under this experiment conditions.

Key words： feeding-forage ramie; silage; rumen fermentation; cellulase activities

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