综述 Review

食品及饲料中黄曲霉毒素生物脱毒的研究进展

展开
  • 东北农业大学动物科学技术学院, 哈尔滨 150030
张立阳(1991-),男,内蒙古赤峰人,博士研究生,研究方向为反刍动物营养。E-mail:zhangliyang91@163.com

收稿日期: 2018-07-09

  网络出版日期: 2019-02-18

基金资助

国家奶牛产业技术体系项目(CARS-36)

Biological Detoxification of Aflatoxin for Food and Feed: A Review

Expand
  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2018-07-09

  Online published: 2019-02-18

摘要

玉米、水稻、高粱、花生等粮食作物常被黄曲霉、寄生曲霉污染,从而导致黄曲霉毒素(AFs)的产生,尤其是被国际癌症研究机构认定为一级致癌物的黄曲霉毒素B1(AFB1)。国内外有大量研究针对饲料中AFB1的脱毒,目前在生产中应用最为广泛、效果最为显著的是在饲料中添加非营养性螯合剂,通过物理结合方式脱毒,但该方法脱毒不彻底、造成饲料营养特性和适口性下降,且引起环境蓄积性污染等缺点日益凸显。生物脱毒因其高效、安全等特性,已成为目前研究的重点。生物脱毒主要包括菌体吸附和生物降解2种方式。乳酸菌中的鼠李糖乳杆菌以及某些酵母菌能够有效地吸附AFs;此外,有大量的细菌、真菌及生物酶对AFs有生物降解作用。本文综述了微生物介导AFs脱毒菌株、酶的种类和脱毒效果,以及相关机理的最新研究进展。

本文引用格式

张立阳, 赵雪娇, 刘帅, 张永根 . 食品及饲料中黄曲霉毒素生物脱毒的研究进展[J]. 动物营养学报, 2019 , 31(2) : 521 -529 . DOI: 10.3969/j.issn.1006-267x.2019.02.005

Abstract

Aspergillus flavus and Aspergillus parasiticus contaminate corn, sorghum, rice, peanuts, corn, rice, sorghum, peanuts and other grain crops. They produce aflatoxins, especially aflatoxin B1 (AFB1), which is classified as a grade 1 carcinogen by the International Agency for Research on Cancer. Many studies have focused on aflatoxin removal from food or feed, dietary supplementation with non-nutritive mycotoxin-sequestering agents is by far the most widely studied method for reducing the effects of mycotoxin exposure. It is detoxified by physical combination. However, the disadvantages of this method include incomplete detoxification, reduced feed nutrition and palatability, and environmental accumulative pollution, which are becoming more and more prominent. Biological detoxification has become the focus of current research because of its high efficiency and safety. Biological detoxification mechanisms-either microbe adsorption or biological degradation. Lactic acid bacteria such as Lactobacillus rhamnosus, and some yeast efficiently binds AFB1. In addition, a large number of bacteria, fungi and biological enzymes have biodegradability to aflatoxin. This review explored the kinds of microbe-associated aflatoxin decontamination microbes and enzymes, detoxification effect and its mechanism.

参考文献

[1] IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.Some traditional herbal medicines,some mycotoxins,naphthalene and styrene[M].IARC monographs on the evaluation of carcinogenic risks to humans.Lyon:IARC Press,2002:1-556.

[2] WALIYAR F,OSIRU M,NTARE B R,et al.Post-harvest management of aflatoxin contamination in groundnut[J].World Mycotoxin Journal,2015,8(2):245-252.  

[3] YITBAREK M B,TAMIR B.Mycotoxines and/or aflatoxines in milk and milk products:review[J].American Scientific Research Journal for Engineering,Technology & Sciences,2014,4(1):1-32.

[4] EL-NEZAMI H,KANKAANPAA P,SALMINEN S,et al.Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen,aflatoxin B1[J].Food and Chemical Toxicology,1998,36(4):321-326.  

[5] KIM S,LEE H,LEE S,et al.Invited review:microbe-mediated aflatoxin decontamination of dairy products and feeds[J].Journal of Dairy Science,2017,100(2):871-880.  

[6] 韩鹏飞,贺稚非,李洪军,等.微生物细胞壁结构及结合真菌毒素的研究进展[J].食品科学,2012,33(11):294-298.

[7] WALTER J.Ecological role of Lactobacilli in the gastrointestinal tract:implications for fundamental and biomedical research[J].Applied and Environmental Microbiology,2008,74(16):4985-4996.  

[8] KIM K,LEE G,THANH H D,et al.Exopolysaccharide from Lactobacillus plantarum LRCC5310 offers protection against rotavirus-induced diarrhea and regulates inflammatory response[J].Journal of Dairy Science,2018,101(7):5702-5712.  

[9] SILVA M,JACOBUS N V,DENEKE C,et al.Antimicrobial substance from a human Lactobacillus strain[J].Antimicrobial Agents and Chemotherapy,1987,31(8):1231-1233.  

[10] PIERIDES M,EL-NEZAMI H,PELTONEN K,et al.Ability of dairy strains of lactic acid bacteria to bind aflatoxin M1 in a food model[J].Journal of Food Protection,2000,63(5):645-650.  

[11] GRATZ S,WU Q K,EL-NEZAMI H,et al.Lactobacillus rhamnosus strain GG reduces aflatoxin B1 transport,metabolism,and toxicity in Caco-2 cells[J].Applied and Environmental Microbiology,2007,73(12):3958-3964.  

[12] DEABES M M,DARWISH H R,ABDEL-AZIZ K B.Protective effects of Lactobacillus rhamnosus GG on aflatoxins-induced toxicities in male albino mice[J].Journal of Environmental and Analytical Toxicology,2012,2(2):2-9.

[13] GRATZ S,TÄUBEL M,JUVONEN R O,et al.Lactobacillus rhamnosus strain GG modulates intestinal absorption,fecal excretion,and toxicity of aflatoxin B1 in rats[J].Applied and Environmental Microbiology,2006,72(11):7398-7400.  

[14] EL-NEZAMI H,KANKAANPÄÄ P,SALMINEN S,et al.Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media[J].Journal of Food Protection,1998,61(4):466-468.  

[15] RAHNAMA P,VOSOUGH A M,SANI A M,et al.In vitro effect of Lactobacillus rhamnosus GG on reduction of aflatoxin B1[J].Nutrition & Food Science,2014,44(1):32-40.  

[16] HASKARD C A,ELNEZAMI H S,KANKAANPÄÄ P E,et al.Surface binding of aflatoxin B1 by lactic acid bacteria[J].Applied and Environmental Microbiology,2001,67(7):3086-3091.  

[17] 刘畅,刘阳,邢福国,等.黄曲霉毒素B1吸附菌株的筛选及吸附机理研究[J].核农学报,2010,24(4):766-771.

[18] LAHTINEN S J,HASKARD C A,OUWEHAND A C,et al.Binding of aflatoxin B1 to cell wall components of Lactobacillus rhamnosus strain GG[J].Food Additives & Contaminants,2004,21(2):158-164.  

[19] STANLEY V G,OJO R,WOLDESENBET S,et al.The use of Saccharomyces cerevisiae to suppress the effects of aflatoxicosis in broiler chicks[J].Poultry Science,1993,72(10):1867-1872.  

[20] DAWSON K A,EVANS J,KUDUPOJE M.Understanding the adsorption characteristics of yeast cell wall preparations associated with mycotoxin binding[C]//LYONS T P,JACQUES K A.Science and technology in the feed industry.Nottingham:Nottinghan University Press,2001:169-182.

[21] TOPCU A,BULAT T,WISHAH R,et al.Detoxification of aflatoxin B1 and patulin by Enterococcus faecium strains[J].International Journal of Food Microbiology,2010,139(3):202-205.  

[22] JURI M G F,DALCERO A M,MAGNOLI C E.In vitro aflatoxin B1 binding capacity by two Enterococcus faecium strains isolated from healthy dog faeces[J].Journal of Applied Microbiology,2015,118(3):574-582.  

[23] HERNANDEZ-MENDOZA A,GUZMAN-DE-PEÑA D,GARCIA H S.Key role of teichoic acids on aflatoxin B1 binding by probiotic bacteria[J].Journal of Applied Microbiology,2009,107(2):395-403.  

[24] 张建梅,李国军,谷巍.生物脱霉剂对黄曲霉毒素B1的作用研究[J].中国饲料,2009(5):21-23.

[25] KASMANI F B,TORSHIZI M A K,ALLAMEH A,et al.A novel aflatoxin-binding Bacillus probiotic:performance,serum biochemistry,and immunological parameters in Japanese quail[J].Poulty Science,2012,91(8):1846-1853.  

[26] KABAK B,VAR I.Factors affecting the removal of aflatoxin M1 from food model by Lactobacillus and Bifidobacterium strains[J].Journal of Environmental Science and Health,Part B:Pesticides,Food Contaminants,and Agricultural Wastes,2008,43(7):617-624.  

[27] GUAN L Z,SUN Y P,CAI J S,et al.The aflatoxin-detoxifizyme specific expression in mouse parotid gland[J].Transgenic Research,2015,24(3):489-496.  

[28] 刘大岭,姚冬生,陈敏峰.真菌提取液对黄曲霉毒素解毒作用的研究[J].广东药学院学报,1995(2):92-94.

[29] ALBERTS J F,GELDERBLOM W C A,BOTHA A,et al.Degradation of aflatoxin B1 by fungal laccase enzymes[J].International Journal of Food Microbiology,2009,135(1):47-52.  

[30] ZHANG W,XUE B B,LI M M,et al.Screening a strain of Aspergillus niger and optimization of fermentation conditions for degradation of aflatoxin B1[J].Toxins,2014,6(11):3157-3172.  

[31] SHCHERBAKOVA L,STATSYUK N,MIKITYUK O,et al.Aflatoxin B1 degradation by metabolites of Phoma glomerata PG41 isolated from natural substrate colonized by aflatoxigenic Aspergillus flavus[J].Jundishapur Journal of Microbiology,2015,8(1):e24324.

[32] WANG J Q,OGATA M,HIRAI H,et al.Detoxification of aflatoxin B1 by manganese peroxidase from the white-rot fungus Phanerochaete sordida YK-624[J].FEMS Microbiology Letters,2011,314(2):164-169.  

[33] HACKBART H C S,MACHADO A R,CHRIST-RIBEIRO A,et al.Reduction of aflatoxins by Rhizopus oryzae and Trichoderma reesei[J].Mycotoxin Research,2014,30(3):141-149.  

[34] MA Q G,GAO X,ZHOU T,et al.Protective effect of Bacillus subtilis ANSB060 on egg quality,biochemical and histopathological changes in layers exposed to aflatoxin B1[J].Poultry Science,2012,91(11):2852-2857.  

[35] GUAN S,JI C,ZHOU T,et al.Aflatoxin B1 degradation by Stenotrophomonas Maltophilia and other microbes selected using coumarin medium[J].International Journal of Molecular Sciences,2008,9(8):1489-1503.  

[36] SUN L H,ZHANG N Y,SUN R R,et al.A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings[J].Microbial Biotechnology,2015,8(3):490-498.  

[37] TENIOLA O D,ADDO P A,BROST I M,et al.Degradation of aflatoxin B1 by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556T[J].International Journal of Food Microbiology,2005,105(2):111-117.  

[38] LAPALIKAR G V,TAYLOR M C,WARDEN A C,et al.F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the actinomycetales[J].PLoS One,2012,7(2):e30114.

[39] ZHAO L H,GUAN S,GAO X,et al.Preparation,purification and characteristics of an aflatoxin degradation enzyme from Myxococcus fulvus ANSM068[J].Journal of Applied Microbiology,2011,110(1):147-155.  

[40] SANGARE L,ZHAO Y J,FOLLY Y M E,et al.Aflatoxin B1 degradation by a Pseudomonas strain[J].Toxins,2014,6(10):3028-3040.  

[41] 李超波,李文明,杨文华,等.黄曲霉毒素B1降解菌的分离鉴定及其降解特性[J].微生物学报,2012,52(9):1129-1136.

[42] KONG Q,ZHAI C P,GUAN B,et al.Mathematic modeling for optimum conditions on aflatoxin B1 degradation by the aerobic bacterium Rhodococcus erythropolis[J].Toxins,2012,4(11):1181-1195.  

[43] LIU D L,YAO D S,LIANG Y Q,et al.Production,purification,and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20)[J].Food and Chemical Toxicology an International Journal,2001,39(5):461-466.  

[44] 刘大岭,姚冬生,黄炳贺,等.黄曲霉毒素解毒酶的固定化及其性质的研究[J].生物工程学报,2003,19(5):603-607.

[45] 陈仪本,蔡斯赞,黄伯爱,等.生物学法降解花生油中黄曲霉毒素的研究[J].卫生研究,1998,27(增刊):81-85.

[46] 王会娟,刘阳,邢福国.高产漆酶平菇的筛选及其在降解黄曲霉毒素B1中的应用[J].核农学报,2012,26(7):1025-1030.

[47] KUSUMANINGTYAS E,WIDIASTUTI R,MARYAM R.Reduction of aflatoxin B1 in chicken feed by using Saccharomyces cerevisiae,Rhizopus oligosporus and their combination[J].Mycopathologia,2006,162(4):307-311.  

[48] DAS A,BHATTACHARYA S,PALANISWAMY M,et al.Biodegradation of aflatoxin B1 in contaminated rice straw by Pleurotus ostreatus MTCC 142 and Pleurotus ostreatus GHBBF10 in the presence of metal salts and surfactants[J].World Journal of Microbiology and Biotechnology,2014,30(8):2315-2324.  

[49] 李俊霞,梁志宏,关舒,等.黄曲霉毒素B1降解菌株的筛选及鉴定[J].中国农业科学,2008,41(5):1459-1463.

[50] 雷元培,赵丽红,马秋刚,等.降解黄曲霉毒素枯草芽孢杆菌的解毒性、抗菌性及抗逆性研究[J].饲料工业,2011,32(24):23-27.

[51] JERMNAK U,CHINAPHUTI A,POAPOLATHEP A,et al.Prevention of aflatoxin contamination by a soil bacterium of Stenotrophomonas sp. that produces aflatoxin production inhibitors[J].Microbiology,2013,159(5):902-912.

[52] TAYLOR M C,JACKSON C J,TATTERSALL D B,et al.Identification and characterization of two families of F420H2-dependent reductases from Mycobacteria that catalyse aflatoxin degradation[J].Molecular Microbiology,2010,78(3):561-575.  

[53] MCCORMICK S P.Microbial detoxification of mycotoxins[J].Journal of Chemical Ecology,2013,39(7):907-918.  

[54] 刘英丽,毛慧佳,刘慧琳,等.黄曲霉毒素B1生物法脱毒机制及产物毒性评价[J].食品工业科技,2018,39(3):324-329.

[55] JI C,FAN Y,ZHAO L H.Review on biological degradation of mycotoxins[J].Animal Nutrition,2016,2(3):127-133.  

[56] 计成,贾如,赵丽红.基因工程技术在黄曲霉毒素生物降解中的应用[J].中国农业科学,2017,50(17):3427-3433.
文章导航

/