动物营养学报 >

2019 , Vol. 31 >Issue 2: 598 - 604

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.015

猪与禽营养 Swine and poultry nutrition

初生重对新生梅山仔猪免疫功能的影响

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  • 扬州大学动物科学与技术学院, 扬州 225009
董丽(1988-),女,江苏海安人,讲师,博士,从事猪的营养与生理调控研究。E-mail:donglijiayou@126.com

收稿日期: 2018-07-31

  网络出版日期: 2019-02-18

基金资助

江苏省自然科学基金青年基金项目（BK20170490）；江苏省科技帮扶苏北专项（SZ-HA2017006）；江苏高校优势学科建设工程资助项目（PAPD）

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Effects of Birth Weight on Immune Function of Meishan Newborn Piglets

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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2018-07-31

  Online published: 2019-02-18

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摘要

本试验旨在研究初生重对新生梅山仔猪免疫功能的影响。选取正常初生重（NBW）和低初生重（LBW）新生梅山仔猪各6头，不喂食母乳，出生6 h内颈动脉放血致死。结果表明：1）LBW组新生梅山仔猪的体重、脾脏重和相对脾脏重均显著低于NBW组（P<0.05）。2）LBW组新生梅山仔猪血液中CD4+ T淋巴细胞比例、CD4+/CD8+均极显著低于NBW组（P<0.01），CD8+ T淋巴细胞比例极显著高于NBW组（P<0.01）。3）LBW组新生梅山仔猪脾脏中T淋巴细胞比例极显著低于NBW组（P<0.01），CD3+ T淋巴细胞比例极显著高于NBW组（P<0.01）。4）LBW组新生梅山仔猪胸腺中白细胞介素-2（IL-2）和白细胞介素-4（IL-4）的基因相对表达量极显著低于NBW组（P<0.01）。5）LBW组新生梅山仔猪脾脏和肠系膜淋巴结中CD8的基因相对表达量显著高于NBW组（P<0.05），IL-2、IL-4和干扰素-γ（IFN-γ）的基因相对表达量极显著高于NBW组（P<0.01）。综上，LBW新生梅山仔猪的免疫功能低于NBW新生梅山仔猪，这与其体内T淋巴细胞分化和功能改变有关。

关键词： 初生重; 梅山仔猪; T淋巴细胞; 细胞因子; 免疫功能

本文引用格式

董丽, 仲召鑫, 毛俊舟, 王淑楠, 王洪荣, 喻礼怀, 包文斌 . 初生重对新生梅山仔猪免疫功能的影响[J]. 动物营养学报, 2019 , 31(2) : 598 -604 . DOI: 10.3969/j.issn.1006-267x.2019.02.015

Abstract

This experiment was conducted to investigate the effects of birth weight on immune function of Meishan newborn piglets. Six normal birth weight (NBW) and 6 low birth weight (LBW) Meishan newborn piglets were no breast milk feeding and killed by jugular vein bloodletting within 6 h of birth. The results showed as follows:1) body weight, spleen weight and relative spleen weight of Meishan newborn piglets in LBW group were significantly lower than those in NBW group (P<0.05). 2) CD4+ T lymphocyte proportion and the ratio of CD4+ to CD8+ T lymphocyte in blood of Meishan newborn piglets in LBW group were extremely significantly lower than those in NBW group (P<0.01), while CD8+ T lymphocyte proportion was extremely significantly higher than that in NBW group (P<0.01). 3) T lymphocyte proportion in spleen of Meishan newborn piglets in LBW group was extremely significantly lower than that in NBW group (P<0.01), while CD3+ T lymphocyte proportion was extremely significantly higher than that in NBW group (P<0.01). 4) The relative gene expression levels of interleukin-2 (IL-2) and interleukin-4 (IL-4) in thymus of Meishan newborn piglets in LBW group were extremely significantly lower than those in NBW group (P<0.01). 5) The relative gene expression level of CD8 in spleen and mesenteric lymph nodes of Meishan newborn piglets in LBW group was significantly higher than that in NBW group (P<0.05), and the relative gene expression levels of IL-2, IL-4 and interferon-γ (IFN-γ) were extremely significantly higher than those in NBW group (P<0.01). In conclusion, immune function of LBW Meishan newborn piglets are lower than those of NBW ones, and this may be associated with changes of differentiation and function of T lymphocyte.

Key words： birth weight; Meishan piglets; T lymphocyte; cytokines; immune function

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