动物营养学报 >

2019 , Vol. 31 >Issue 2: 809 - 823

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.037

饲料科学与技术 Feed science and technology

姜黄素对草鱼生长性能、抗氧化应激能力及核因子E2相关因子2/抗氧化反应元件信号通路相关基因表达的影响

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  • 1. 浙江省水生生物资源养护与开发技术研究重点实验室, 中国水产科学研究院水生动物繁育与营养重点实验室, 湖州师范学院生命科学学院, 湖州 313000;
    2. 农业部淡水渔业和种质资源利用重点实验室, 中国水产科学研究院淡水渔业研究中心, 无锡 214081
明建华(1973-),男,湖北黄石人,副教授,博士,从事水产动物营养与免疫、抗应激等方面研究。E-mail:mingjianhua686@163.com

收稿日期: 2018-08-20

  网络出版日期: 2019-02-18

基金资助

国家自然科学基金项目（31372544）；农业部淡水渔业和种质资源利用重点实验室开放课题（KF201302，KF201506）；现代农业产业技术体系建设专项（CARS-45-11）；浙江省重点研发计划项目（2015C03018）；湖州市公益性技术应用重点项目（2015GZ07）

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Effects of Curcumin on Growth Performance, Oxidative Stress Resistance and Expression of Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Responsive Element Signaling Pathway-Related Genes in Grass Carp (Ctenopharyngodon idella)

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  • 1. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences, College of Life Science, Huzhou University, Huzhou 313000, China;
    2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Received date: 2018-08-20

  Online published: 2019-02-18

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摘要

本试验旨在研究姜黄素对草鱼生长性能、抗氧化应激能力以及核因子E2相关因子2/抗氧化反应元件（Nrf2/ARE）信号通路相关基因表达的影响。选择初体质量为（5.11±0.08）g的草鱼幼鱼525尾，随机分为5组（每组3个重复，每个重复35尾），分别饲喂在基础饲料中添加0（对照）、200、400、600和800 mg/kg姜黄素的5种饲料。试验期60 d。饲养试验结束后，每重复取15尾规格基本一致的草鱼，每尾鱼腹腔注射10 mg/kg BW敌草快，进行急性氧化应激试验，在注射前（0 h）、注射后24 h时分别采样，检测草鱼血清和肝脏相关的生理生化指标，以及肝脏抗氧化相关基因的mRNA表达水平。结果表明：1）随着饲料中姜黄素添加量的增加，草鱼幼鱼的增重率、特定生长率和成活率均先升高后下降，而饲料系数和肝体指数则先下降后升高，当饲料中姜黄素添加量为400、600 mg/kg时草鱼的生长性能较佳，而脏体指数和肥满度在各组间的差异均不显著（P>0.05）。2）在草鱼注射敌草快前，其血清皮质醇（COR）、葡萄糖（GLU）含量及谷丙转氨酶（ALT）和谷草转氨酶（AST）活性在各组间均无显著差异（P>0.05）；在注射敌草快24 h后，草鱼血清上述指标随着饲料中姜黄素添加量的增加呈先下降后升高的变化趋势，当饲料姜黄素添加量为400、600 mg/kg时达到较低水平，显著低于对照组（P<0.05）。3）在草鱼注射敌草快前、注射24 h后，随着饲料中姜黄素添加量的增加，草鱼肝脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性，总抗氧化能力（T-AOC）和还原型谷胱甘肽（GSH）含量，Nrf2、铜与锌超氧化物歧化酶（Cu-ZnSOD）、锰超氧化物歧化酶（MnSOD）、CAT、GSH-Px和诱导型热休克蛋白70（HSP70）mRNA表达水平均呈先升高后下降的变化趋势，而肝脏活性氧（ROS）、丙二醛（MDA）和Kelch样环氧氯丙烷相关蛋白1（Keap1）mRNA表达水平均呈先下降后升高的变化趋势，当饲料姜黄素添加量为400、600 mg/kg时，这些指标分别达到较高或较低的水平。由此可见，饲料中添加姜黄素400、600 mg/kg可促进草鱼幼鱼的生长，降低饲料系数，提高鱼体抗氧化应激能力，对敌草快诱导的急性氧化应激损伤具有保护作用，并可激活Nrf2/ARE信号通路相关基因的表达。

关键词： 草鱼; 姜黄素; 生长; 抗氧化应激能力; 基因表达

本文引用格式

明建华, 叶金云, 张易祥, 杨霞, 邵仙萍, 强俊, 徐跑 . 姜黄素对草鱼生长性能、抗氧化应激能力及核因子E2相关因子2/抗氧化反应元件信号通路相关基因表达的影响[J]. 动物营养学报, 2019 , 31(2) : 809 -823 . DOI: 10.3969/j.issn.1006-267x.2019.02.037

Abstract

The present study aimd to investigate the effects of curcumin on growth performance, oxidative stress resistance and expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant responsive element (ARE) signaling pathway-related genes in grass carp (Ctenopharyngodon idella). Five hundred and twenty-five juvenile grass carp with an initial body weight of (5.11±0.08) g were randomly distributed into five groups with three replicates each, fed five diets supplemented with graded levels of curcumin[0 (control), 200, 400, 600 and 800 mg/kg diet] for 60 days. At the end of the feeding experiment, fifteen fish per tank were injected intraperitoneally with 10 mg diquat/kg body weight (BW) to carry out acute oxidative stress test. The blood and liver of grass carp were sampled before (0 h) and after 24 h of injection, and their physiological and biochemical indicators, as well as the mRNA expression levels of liver antioxidant-related genes were detected. The results showed as follows:1) with the increase of dietary curcumin supplementation, the weight gain rate (WGR), specific growth rate (SGR) and survival rate of juvenile grass carp increased first and then decreased, while the feed conversion ratio (FCR) and hepatosomatic index (HSI) decreased first and then increased; the growth performance of grass carp was better when diets supplemented with 400, 600 mg/kg curcumin. There was no significant difference in viscerosomatic index (VSI) and condition factor (CF) among the experimental groups (P>0.05). 2) Before diquat injection, there was not significantly different in serum cortisol (COR), glucose (GLU) contents, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities among groups (P>0.05). After 24 h of diquat injection, above indicators in serum decreased first and then increased, and which reached the lower levels when dietary curcumin supplementation was 400, 600 mg/kg and were lower than those in the control group (P<0.05). 3) Befor and after 24 h of diquat injection, with the increase of dietary curcumin supplementation, liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC) and glutathione (GSH) content, the mRNA expression levels of Nrf2, copper and zinc superoxide dismutase (Cu-ZnSOD), manganese superoxide dismutase (MnSOD), CAT and GSH-Px and heat shock protein 70 (HSP70) genes increased first and then decreased, while liver reactive oxygen species (ROS) and malondialdehyde (MDA) content, and mRNA expression level of Kelch-like ECH-associated protein1 (Keap1) trended to decrease first and then increase. When diet supplemented with 400, 600 mg/kg curcumin, these indexes reached the higher of lower levels, respectively. Therefore, the diet supplemented with 400, 600 mg/kg curcumin can promote the growth of juvenile grass carp, reduce FCR, improve antioxidative stress ability, and protect fish against acute oxidative stress injury induced by diquat; can activate the Nrf2/ARE signaling pathway of grass carp.

Key words： grass carp (Ctenopharyngodon idella); curcumin; growth performance; oxidative stress resistance; gene expression

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