饲料科学与技术 Feed science and technology

刺槐豆胶酶解产物对肉仔鸡生长性能、血清生化指标和抗氧化能力的影响

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  • 1. 中国农业科学院饲料研究所, 生物饲料开发国家工程研究中心, 农业部饲料生物技术重点实验室, 北京 100081;
    2. 中国农业科学院特产研究所, 长春 130112
谢静静(1994-),女,陕西宝鸡人,硕士研究生,研究方向为单胃动物营养。E-mail:1440674628@qq.com

收稿日期: 2018-07-09

  网络出版日期: 2019-02-18

基金资助

北京市科技计划"饲用微生物与酶制剂检测技术研究与应用"(D161100006116002);国家重点研发计划资助(2018YFD0500600)

Effects of Locust Bean Gum Enzymatic Hydrolysate on Growth Performance, Serum Biochemical Indexes and Antioxidant Capacity of Broilers

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  • 1. Key Laboratory for Feed Biotechnology of Ministry of Agriculture, Institute of Feed Research, National Engineering Research Center of Biological Feed, Chinese Academy of Agriculture Sciences, Beijing 100081, China;
    2. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2018-07-09

  Online published: 2019-02-18

摘要

本试验旨在研究饲粮添加不同水平刺槐豆胶酶解产物对肉仔鸡生长性能、血清生化指标和抗氧化能力的影响。选用1日龄健康的爱拔益加(AA)肉仔鸡768羽,随机分为6个组,每组8个重复,每个重复16只鸡。Ⅰ组饲喂基础饲粮,Ⅱ组在基础饲粮中添加有效成分含量为5 mg/kg的黄霉素,Ⅲ组在基础饲粮中添加0.1%的刺槐豆胶,Ⅳ、Ⅴ和Ⅵ组分别在基础饲粮中添加0.1%、0.2%和0.3%的刺槐豆胶酶解产物。试验期42 d。结果表明:1)1~21日龄,Ⅵ组平均日增重(ADG)显著高于Ⅰ组和Ⅲ组(P<0.05),Ⅴ组和Ⅵ组平均日采食量(ADFI)显著高于Ⅰ组(P<0.05)。22~42日龄,Ⅴ组ADG显著高于Ⅲ组和Ⅳ组(P<0.05)。1~42日龄,Ⅴ组和Ⅵ组ADG和ADFI均显著高于Ⅲ组(P<0.05)。2)21和42日龄,各组之间血清总蛋白(TP)、白蛋白(ALB)、胆固醇(CHO)、甘油三酯(TG)、葡萄糖(GLU)含量及谷草转氨酶(AST)、谷丙转氨酶(ALT)活性均无显著差异(P>0.05)。3)21和42日龄,各组之间血清谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)、谷胱甘肽还原酶(GR)活性和丙二醛(MDA)含量均无显著差异(P>0.05)。各组之间胸肌T-SOD、GR活性和MDA含量无显著差异(P>0.05)。Ⅵ组肝脏MDA含量显著低于Ⅱ组和Ⅲ组(P<0.05),Ⅵ组肝脏T-SOD活性显著高于Ⅰ组、Ⅲ组和Ⅳ组(P<0.05)。综上所述,饲粮中添加适量刺槐豆胶酶解产物可以提高肉仔鸡ADG和ADFI,增强肝脏抗氧化能力。本试验条件下,肉仔鸡饲粮中刺槐豆胶酶解产物的适宜添加量为0.3%。

本文引用格式

谢静静, 王中成, 崔虎, 乜豪, 张铁涛, 高秀华, 乔宇 . 刺槐豆胶酶解产物对肉仔鸡生长性能、血清生化指标和抗氧化能力的影响[J]. 动物营养学报, 2019 , 31(2) : 883 -891 . DOI: 10.3969/j.issn.1006-267x.2019.02.045

Abstract

The aim of this study was to investigate the effects of different dietary locust bean gum enzymatic hydrolysate levels on growth performance, serum biochemical indexes and antioxidant capacity of broilers. A total of 768 healthy one-day-old Arbor Acres (AA) broilers were randomly divided into 6 groups with 8 replicates per group and 16 broilers per replicate. Broilers in group Ⅰ were fed a basal diet, broilers in group Ⅱ were fed the basal diet supplemented with an active ingredient content 5 mg/kg flavomycin, broilers in group Ⅲ were fed the basal diet supplemented with 0.1% locust bean gum, and broilers in groups Ⅳ, Ⅴ and Ⅵ were fed the basal diet supplemented with 0.1%, 0.2% and 0.3% locust bean gum enzymatic hydrolysate, respectively. The experimental period was 42 days. The results showed as follows:1) during 1 to 21 days of age, the average daily gain (ADG) of group Ⅵ was significantly higher than that of groups Ⅰ and Ⅲ (P<0.05), the average daily feed intake (ADFI) of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅰ (P<0.05). During 22 to 42 days of age, the ADG of group Ⅴ was significantly higher than that of groups Ⅲ and Ⅳ (P<0.05). During 1 to 42 days of age, the ADG and ADFI of groups Ⅴ and Ⅵ were significantly higher than those of group Ⅲ (P<0.05). 2) On 21 and 42 days of age, there were no significant differences in the contents of total protein (TP), albumin (ALB), cholesterol (CHO), triglyceride (TG), glucose (GLU) and activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum among all groups (P>0.05). 3) On 21 and 42 days of age, there were no significant differences in the activities of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), glutathione reductase (GR) and content of malondialdehyde (MDA) in serum among all groups (P>0.05). There were no significant differences in the activities of T-SOD, GR and content of MDA in serum among all groups (P>0.05). The liver MDA content of group Ⅵ was significantly higher than that of groups Ⅱ and Ⅲ (P<0.05), the liver T-SOD activity of group Ⅵ was significantly higher than that of groups Ⅰ, Ⅱ and Ⅲ (P<0.05). In conclusion, the dietary appropriate addition of locust bean gum enzymatic hydrolysate can improve the ADG and ADFI of broilers, enhance liver antioxidant capacity. In this experiment, the suitable dietary locust bean gum enzymatic hydrolysate addition of broilers is 0.3%.

参考文献

[1] GIBSON G R,ROBERFROID M B.Dietary modulation of the human colonic microbiota:introducing the concept of prebiotics[J].The Journal of Nutrition,1995,125(6):1401-1412.  

[2] CAMACHO M M,MARTINEZ-NAVARRETE N,CHIRALT A.Rheological characterization of experimental dairy creams formulated with locust bean gum (LBG) and λ-carrageenan combinations[J].International Dairy Journal,2005,15(3):243-248.  

[3] 杨永利,张继,郭守军,等.槐豆胶的急性毒性试验及槐豆胶与长角豆胶、瓜胶流变性的比较研究[J].西北师范大学学报(自然科学版),1997,33(4):52-54.

[4] BRUFAU M T,MARTÍN-VENEGAS R,GUERRERO-ZAMORA A M,et al.Dietary β-galactomannans have beneficial effects on the intestinal morphology of chickens challenged with Salmonella enterica serovar Enteritidis[J].Journal of Animal Science,2015,93(1):238-246.  

[5] NUYENS F,SMEETS N,VAN CAMPENHOUT L,et al.Determination of non-starch polysaccharides in wheat by NIRS for the prediction of the nutritional value of feed enzymes in broilers[C]//World's Poultry Congress.Salvador:[s.n.],2012.

[6] ANNISON G,HUGHES R J,CHOCT M.Effects of enzyme supplementation on the nutritive value of dehulled lupins[J].British Poultry Science,1996,37(1):157-172.  

[7] SMITS C H M,ANNISON G.Non-starch plant polysaccharides in broiler nutrition-towards a physiologically valid approach to their determination[J].World's Poultry Science Journal,1996,52(2):203-221.  

[8] KAKANI R.Prebiotic properties of yeast cell wall mannanoligosaccharides and guar gum galactomannans in starting broilers[D]. Doctor thesis.Texas:Texas A & M University,2013.

[9] 王彬,黄瑞林,印遇龙,等.半乳甘露寡糖对育肥猪的应用效果[J].中国科学院研究生院学报,2006,23(3):364-369.

[10] 王彬,黄瑞林,何子双,等.半乳甘露寡糖取代抗生素对泌乳母猪的应用效果[J].安徽农业科学,2006,34(3):483-484,605.

[11] ISHIHARA N,CHU D C,AKACHI S,et al.Preventive effect of partially hydrolyzed guar gum on infection of Salmonella enteritidis in young and laying hens[J].Poultry Science,2000,79(5):689-697.  

[12] KIM K W,THOMAS R L.Antioxidative activity of chitosans with varying molecular weights[J].Food Chemistry,2007,101(1):308-313.  

[13] 肖宇,王利华,孙国强,等.外源寡糖对奶山羊血清生化指标和抗氧化指标的影响[J].动物营养学报,2012,24(2):342-348.

[14] BRUFAU M T,GUARDIOLA F,BOU R,et al.β-Galactomannans protect epithelial barrier function disruption induced by Salmonella enteritidis in human intestinal Caco-2 cells[J].Proceedings of the Nutrition Society,2013,72:E51.

[15] CARNÉ S,ZARAGOZA A.β-galactomananos vegetales hidrolizados para prevenir la invasión intestinal de Salmonella en avicultura[J].Selecciones Avícolas,2014,56(9):37-41.  

[16] VILÀ B,DE QUEIROZ D,BADIOLA I,et al.Effects of carob bean gum on performance,nutrient digestibility and Salmonella enterica var. Enteritidis colonisation in chickens[J].Food Research International,2012,45(2):1133-1138.  

[17] ZHANG C.Evaluation of guar meal as a source of prebiotic galactomannans for laying hens[D].Doctor thesis.Texas:Texas A & M University,2004.

[18] MAINAR-JAIME R C,ANDRÉS S,AUSERÉ M,et al.Galactomannans for the control of Salmonella infection in fattening pigs[C]//Proceedings of the 10th International Conference on the Epidemiology and Control of Biological,Chemical and Physical Hazards in Pigs and Pork. Ames:Iowa State University Digital Press,2013.

[19] SCHALINSKE L.Impact of β-galactomannan on health status and immune function in rats[D]. Doctor thesis.Ames:Iowa State University,2015.

[20] BADIA R,LIZARDO R,MARTÍNEZ P,et al.Oligosaccharide structure determines prebiotic role of β-galactomannan against Salmonella enterica ser. Typhimurium in vitro[J].Gut Microbes,2013,4(1):72-75.  

[21] BADIA R,BRUFAU M T,GUERRERO-ZAMORA A M,et al.β-galactomannan and Saccharomyces cerevisiae var. boulardii modulate the immune response against Salmonella enterica serovar Typhimurium in porcine intestinal epithelial and dendritic cells[J].Clinical and Vaccine Immunology,2012,19(3):368-376.  

[22] BADIA R,ZANELLO G,CHEVALEYRE C,et al.Effect of Saccharomyces cerevisiae var. Boulardii and β-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4(K88)[J].Veterinary Research,2012,43:4.

[23] JUNEJA L R,SAKANAKA S,CHU D C.Physiological and technological functions of partially hydrolysed guar gum (modified galactomannans)[M]//MC CLEARY B V,PROSKY L.Advanced dietary fibre technology.Oxford:Blackwell Science Ltd,2008.

[24] GIANNINI E G,MANSI C,DULBECCO P,et al.Role of partially hydrolyzed guar gum in the treatment of irritable bowel syndrome[J].Nutrition,2006,22(3):334-342.  

[25] RAJANI J,DASTAR B,SAMADI F,et al.Effect of extracted galactoglucomannan oligosaccharides from Pine wood (Pinus brutia) on Salmonella typhimurium colonisation,growth performance and intestinal morphology in broiler chicks[J].British Poultry Science,2016,57(5):682-692.

[26] 杜冰,吴襟,张敏,等.半乳甘露寡糖对肉兔生长性能、屠宰性能和胃肠道pH值的影响[J].饲料工业,2006,27(8):39-41.

[27] 李鹏,佘锐萍,吴襟,等.半乳甘露寡糖对兔小肠黏膜结构及免疫相关细胞数量的影响[J].中国兽医杂志,2007,43(4):19-20.

[28] 张彩云,高天增,李德发,等.半乳甘露寡糖对早期断奶仔猪生长性能的影响[J].饲料研究,2003(3):1-3.

[29] 胡静,林英庭.半乳甘露寡糖在动物生产中的研究进展[J].饲料博览,2012(9):29-31.

[30] 王锐,刘军,刘辉宇,等.乳甘露寡糖对异育银鲫幼鱼生长和非特异性免疫的影响[J].上海水产大学学报,2008,17(4):502-506.

[31] WANG R L,HOU Z P,WANG B,et al.Effects of feeding galactomannan oligosaccharides on growth performance,serum antibody levels and intestinal microbiota in newly-weaned pigs[J].Journal of Food Agriculture & Environment,2010,8(3):47-55.

[32] 王吉潭,李德发,龚利敏,等.半乳甘露寡糖对肉鸡生产性能和免疫机能的影响[J].中国畜牧杂志,2003,39(2):5-7.

[33] 陈建荣.不同营养水平日粮中添加半乳甘露寡糖对哺乳母猪生产性能及免疫机能的影响[D].硕士学位论文.长沙:湖南农业大学,2007.

[34] IDE T,MORIUCHI H,NIHIMOTO K.Hypolipidemic effects of guar gum and its enzyme hydrolysate in rats fed highly saturated fat diets[J].Annals of Nutrition & Metabolism,1991,35(1):34-44.  

[35] 王际英,宋志东,李培玉,等.饲料添加半乳甘露寡糖对刺参幼参生长、体壁营养组成及免疫力的影响[J].中国水产科学,2014,21(2):310-319.
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