本试验旨在探讨全波长酶标仪代替紫外分光光度计测定多糖含量的可行性，并对其测定结果的可靠性进行验证。首先，分别用紫外分光光度计和全波长酶标仪检测葡萄糖标准溶液葡萄糖含量，确认全波长酶标仪可替代紫外分光光度计进行多糖含量检测；随后，考察全波长酶标仪检测多糖含量的可靠性并对测定条件进行优化；最后，使用该方法对天然植物复方粗提取物的多糖含量进行检测。结果表明：全波长酶标仪可代替紫外分光光度计测定多糖含量，该方法精密度相对标准偏差（RSD）值为0.41%，样品重复性RSD值为1.06%，标准品重复性RSD值为0.84%，回收率100.02%，样品稳定性RSD值为1.25%，标准品稳定性RSD值为1.34%。对主要影响因素稀释倍数、检测波长、硫酸添加量和水浴温度进行筛选，最终确定全波长酶标仪-苯酚-硫酸法测定天然植物复方多糖中多糖含量的测定条件为：天然植物复方多糖样品经处理制成母液后稀释200倍、硫酸添加量5 mL、水浴温度30℃、检测波长490 nm。经测定，天然植物复方多糖中多糖含量为114.70 mg/g。

This study aims to investigate the feasibility of measuring the polysaccharide content by a full-wavelength microplate reader instead of the ultraviolet spectrophotometer, and to verify the reliability of the results. First, the glucose content of the glucose standard solution was detected by ultraviolet spectrophotometer and full-wavelength microplate reader, respectively, to confirm the feasibility of measuring the polysaccharide content by a full-wavelength microplate reader instead of the ultraviolet spectrophotometer. Then, the reliability of the full-wavelength microplate reader for detecting polysaccharide content was investigated and the measurement conditions were optimized. Finally, using this method to detect the polysaccharide content of natural plant polysaccharide compound. The results showed that the full-wavelength microplate reader could replace the ultraviolet spectrophotometer to determine the polysaccharide content. For full-wavelength microplate reader method, the relative standard deviation (RSD) value of precision was 0.41%, the RSD value of sample repeatability was 1.06%, the RSD value of standard repeatability was 0.84%, the recovery rate was 100.02%, the RSD value of sample stability was 1.25%, and the RSD value of standard stability was 1.34%. The main influencing factors including dilution multiple, detection wavelength, sulfuric acid addition and water bath temperature were screened. The determination conditions of polysaccharide content in compound polysaccharides of natural plant polysaccharide compound by full-wavelength microplate reader-phenol-sulfuric acid method were as follows:sample was treated and diluted 200 times into a testing solution, determined as 5 mL of sulfuric acid, 30℃ water bath temperature, and 490 nm detection wavelength. Through measuring, the polysaccharide content in the natural plant polysaccharide compound is 114.70 mg/g.