饲料科学与技术 Feed science and technology

饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响

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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 成都 611130
毛湘冰(1980-),男,湖南长沙人,副教授,博士,研究方向为动物营养与饲料科学。E-mail:acatmxb2003@163.com

收稿日期: 2018-08-14

  网络出版日期: 2019-03-18

基金资助

现代农业产业技术体系(CARS-35);四川省科技支撑项目(2016NYZ0052,2016NZ0006)

Effects of Dietary Pectic Oligosaccharide on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus

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  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-08-14

  Online published: 2019-03-18

摘要

本研究旨在探讨饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响。试验选取28头健康的21日龄断奶的"杜×长×大"仔猪,采用2×2因子试验设计,即果胶寡糖处理(饲粮添加200 mg/kg果胶寡糖或不添加)和轮状病毒攻毒(灌服轮状病毒或无菌培养液),轮状病毒攻毒于试验第15天早上进行。试验期18 d。结果表明:轮状病毒攻毒提高了断奶仔猪的腹泻率和回肠黏膜中轮状病毒非结构蛋白4(NSP4)的阳性率,显著降低了回肠黏膜的绒毛高度和绒毛高度/隐窝深度值(P<0.05),显著降低了回肠黏膜中闭锁小带蛋白-1(ZO-1)、闭合蛋白(occludin)、黏蛋白(mucin)1和mucin2的表达(P<0.05),显著提高了回肠黏膜中丙二醛的含量和回肠食糜中大肠杆菌的数量(P<0.05),并显著降低了回肠黏膜中总抗氧化能力和回肠食糜中乳酸杆菌和总菌的数量(P<0.05);而饲粮添加果胶寡糖降低了轮状病毒攻毒断奶仔猪的腹泻率和回肠黏膜中轮状病毒NSP4的阳性率,并显著缓解了轮状病毒对断奶仔猪回肠形态结构、回肠黏膜中occludin、mucin1和mucin2的表达和抗氧化能力以及回肠食糜中微生物数量的影响(P<0.05)。综上所述,在断奶仔猪饲粮中添加果胶寡糖可以预防轮状病毒诱导发生的腹泻,而这一作用与其可改善回肠屏障功能有关。

本文引用格式

毛湘冰, 陈浩, 陈代文, 余冰, 何军, 郑萍, 虞洁, 罗钧秋, 罗玉衡 . 饲粮添加果胶寡糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响[J]. 动物营养学报, 2019 , 31(3) : 1288 -1294 . DOI: 10.3969/j.issn.1006-267x.2019.03.034

Abstract

This experiment was conducted to determine the effects of dietary pectic oligosaccharide (POS) on the ileal barrier function of weaned pigs challenged by rotavirus (RV). Twenty-eight healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included POS treatment (control diet or supplementing 200 mg/kg POS diet) and RV challenge (oral gavage with RV or sterilized medium). On day 15, RV challenge was carried out. The trial duration was 18 days. The results showed that RV infection increased the diarrhea rate and positive rate of rotavirus non-structural protein 4 (NSP4) in ileal mucosa, significantly decreased the villus height and villus height to crypt depth ratio of ileal mucosa (P<0.05), significantly reduced the expressions of zonula occludens-1 (ZO-1), occludin, mucin 1 and mucin 2 in ileal mucosa (P<0.05), significantly enhanced the malondialdehyde content in ileal mucosa and E. coli population in ileal digesta (P<0.05), and significantly decreased total antioxidant capacity in ileal mucosa and the population of Lactobacillus and total bacteria in ileal digesta of weaned pigs (P<0.05). Dietary POS reduced the diarrhea rate and positive rate of rotavirus NSP4 in ileal mucosa of the RV-infected weaned piglets, and significantly alleviated the effects of RV challenge on ileal mucosa morphology, microbial population in ileal digesta, antioxidant capacity and expressions of occludin, mucin 1 and mucin 2 in ileal mucosa of weaned piglets (P<0.05). These results suggest that dietary POS can prevent the RV-induced diarrhea, which is associated with the improvement of ileal barrier function of weaned pigs.

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