饲料科学与技术 Feed science and technology

混合真菌发酵对玉米秸秆纤维素与木质素降解率的影响

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  • 黑龙江八一农垦大学动物科技学院, 大庆 163319
张仲卿(1992-),男,辽宁绥中人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:980890780@qq.com

收稿日期: 2018-08-18

  网络出版日期: 2019-03-18

基金资助

国家重点研发计划项目(2018YFD0502102);黑龙江省科技厅中央引导地方项目(ZY17C08);黑龙江省农垦总局十三五科技攻关项目(HNK135-04-03,HNK135-07-03)

Effects of Mixed Fungal Fermentation on Degradation Rate of Cellulose and Lignin of Corn Straw

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  • College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2018-08-18

  Online published: 2019-03-18

摘要

本试验以拟康宁木霉(Trichoderma koningiopsis)和黄孢原毛平革菌(Phanerochaete chrysosporium)为发酵菌种,研究混合发酵对玉米秸秆纤维素和木质素降解率的影响及最优发酵条件。采用单因素试验设计,通过测定菌种比例(拟康宁木霉:黄孢原毛平革菌)、接种量、营养液添加量及发酵天数对纤维素和木质素降解率的影响,筛选出混合真菌发酵的最优条件,并进一步采用响应面法评价各因素及其交互作用对玉米秸秆纤维素和木质素降解率的影响,最后采用Design Expert 8.0.6软件分别建立混合真菌发酵玉米秸秆降解纤维素和木质素条件的二次多项式数学模型。结果表明:混合真菌发酵玉米秸秆的最优发酵条件为菌种比例1.0:4.1,接种量25.2%,营养液添加量72.2%,发酵天数11 d。在此条件下,玉米秸秆纤维素和木质素降解率分别达到36.80%和28.87%。

本文引用格式

张仲卿, 张爱忠, 姜宁 . 混合真菌发酵对玉米秸秆纤维素与木质素降解率的影响[J]. 动物营养学报, 2019 , 31(3) : 1385 -1395 . DOI: 10.3969/j.issn.1006-267x.2019.03.046

Abstract

In this experiment, Trichoderma koningiopsis and Phanerochaete chrysosporium were used as fermentation strains, the effects of mixed fungal fermentation on degradation rate of cellulose and lignin of corn straw and the optimal fermentation parameters were investigated. Single factor experiment was used to study the effects of the fungus ratio (Trichoderma koningiopsis:Phanerochaete chrysosporium), inoculation amount, additive amount of nutrient solution and fermentation day on the degradation rate of cellulose and lignin. On the basis of this experiment, the optimal fermentation factors were selected, and the effects of various factors and their interactions on the degradation rates of cellulose and lignin were further evaluated by response surface method. The quadratic polynomial mathematical model of degradation rates of cellulose and lignin of corn straw by mixed fungus fermentation was established by using design expert 8.0.6 software. The results show that the optimal parameters of mixed fungus fermentation of corn straw are as follows:fungus ratio 1.0:4.1, inoculation amount 25.2%, additive amount of nutrient solution 72.2%, fermentation days 11 days. On this condition, the degradation rates of cellulose and lignin reach 36.80% and 28.87%, respectively.

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