姚静静, 胡骁飞, 韩俊岭, 徐帆, 滕蔓, 邢云瑞, 孙亚宁, 邓瑞广, 张改平 . 黄曲霉毒素B₁单克隆抗体的制备及基于该抗体的黄曲霉毒素B₁免疫学检测方法的建立[J]. 动物营养学报, 2019 , 31(3) : 1405 -1414 . DOI: 10.3969/j.issn.1006-267x.2019.03.048

[1] BENNETT J W,KLICH M.Mycotoxins[J].Clinical Microbiology Reviews,2003,16(3):497-516.  

[2] MACHIDA M,ASAI K,SANO M,et al.Genome sequencing and analysis of Aspergillus oryzae[J].Nature,2005,438(7071):1157-1161.  

[3] WU F,GROOPMAN J D,PESTKA J J.Public health impacts of foodborne mycotoxins[J].Annual Review of Food Science and Technology,2014,5:351-372.

[4] 陈兴祥,黄克和.黄曲霉毒素对畜禽免疫机能的影响[J].中国兽医杂志,2002,38(10):33-35.

[5] OSTRY V,MALIR F,TOMAN J,et al.Mycotoxins as human carcinogens-the IARC monographs classification[J].Mycotoxin Research,2017,33(1):65-73.  

[6] IARC.International Agency for Research on Cancer,WHO IARC Monographs on the evaluation of carcinogenic risks to humans Some traditional herbal medicines,some mycotoxins naphthalene and styrene aflatoxins[C].Lyon:IARC, 2002.

[7] CHOI S,JUN H,BANG J,et al.Behaviour of Aspergillus flavus and Fusarium graminearum on rice as affected by degree of milling,temperature,and relative humidity during storage[J].Food Microbiology,2015,46:307-313.

[8] HU X F,HU R,ZHANG Z W,et al.Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS[J].Analytical and Bioanalytical Chemistry,2016,408(22):6027-6036.  

[9] GARCÍA-MORALEJA A,FONT G,MAÑES J,et al.Development of a new method for the simultaneous determination of 21 mycotoxins in coffee beverages by liquid chromatography tandem mass spectrometry[J].Food Research International,2015,72:247-255.

[10] ZHENG W L,TENG J,CHENG L,et al.Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B₁ using an electrochemical aptasensor[J].Biosensors and Bioelectronics,2016,80:574-581.

[11] GARCÍA-FONSECA S,BALLESTEROS-GÍMEZ A,RUBIO S.Restricted access supramolecular solvents for sample treatment in enzyme-linked immuno-sorbent assay of mycotoxins in food[J].Analytica Chimica Acta,2016,935:129-135.

[12] LEE N A,WANG S,ALLAN R D,et al.A rapid aflatoxin B₁ ELISA:development and validation with reduced matrix effects for peanuts,corn,pistachio,and soybeans[J].Journal of Agricultural and Food Chemistry,2004,52(10):2746-2755.  

[13] HU X,YAO J,WANG F,et al.Eu³⁺-labeled IgG-based time-resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed[J].Journal of the Science of Food and Agriculture,2018,98(2):674-680.  

[14] WANG D,ZHANG Z W,LI P W,et al.Europium nanospheres-based time-resolved fluorescence for rapid and ultrasensitive determination of total aflatoxin in feed[J].Journal of Agricultural and Food Chemistry,2015,63(47):10313-10318.  

[15] HANG D H,LI P W,ZHANG Q,et al.Ultrasensitive nanogold probe-based immunochromatographic assay for simultaneous detection of total aflatoxins in peanuts[J].Biosensors and Bioelectronics,2010,26(6):2877-2882.

[16] LI P W,ZHANG Z W,ZHANG Q,et al.Current development of microfluidic immunosensing approaches for mycotoxin detection via capillary electromigration and lateral flow technology[J].Electrophoresis,2012,33(15):2253-2265.  

[17] CHEN Y Q,CHEN Q,HAN M M,et al.Development and optimization of a multiplex lateral flow immunoassay for the simultaneous determination of three mycotoxins in corn,rice and peanut[J].Food Chemistry,2016,213:478-484.

[18] KOLOSOVA A Y,SHIM W B,YANG Z Y,et al.Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B₁.Stabilization of ELISA kit components and application to grain samples[J].Analytical and Bioanalytical Chemistry,2006,384(1):286-294.  

[19] 王磊,胡骁飞,滕蔓,等.抗黄曲霉毒素B₁单抗制备及免疫学定量方法建立[J].中国公共卫生,2012,28(1):58-60.

[20] 谢珲,章先,王歆,等.黄曲霉毒素B₁单克隆抗体的制备及间接竞争ELISA检测技术研究[J].微生物学通报,2015,42(10):2033-2040.

[21] KONG D Z,XIE Z J,LIU L Q,et al.Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of vancomycin in raw milk and animal feed[J].Food and Agricultural Immunology,2017,28(3):414-426.  

[22] 宋青龙,李成洪,傅巍,等.黄曲霉毒素B₁胶体金快速定量检测试剂盒的研发[J].动物营养学报,2017,29(10):3703-3709.

[23] KEMP H A,MORGAN M R A.Studies on the detrimental effects of bivalent binding in a microtitration plate ELISA and possible remedies[J].Journal of Immunological Methods,1986,94(1/2):65-72.

[24] 王磊,胡骁飞,职爱民,等.黄曲霉毒素B₁人工抗原及鼠源多克隆抗血清的制备[J].食品科技,2011,36(3):277-281.

[25] LANE R D,CRISSMAN R S,GINN S.High efficiency fusion procedure for producing monoclonal antibodies against weak immunogens[J].Methods in Enzymology,1986,121:183-192.

[26] 刘晓波,蔡美英,王霞,等.一种简单实用纯化腹水McAb方法——辛酸/硫酸铵法[J].华西医科大学学报,1999,30(4):455-456.

[27] BEATTY J D,BEATTY B G,VLAHOS W G.Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay[J].Journal of Immunological Methods,1987,100(1/2):173-179.

[28] 万文徽.单克隆抗体亲和常数的测定[J].单克隆抗体通讯,1993,9(2):72-75.

[29] YAO J J,WANG F Y,HAN J L,et al.Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots[J].Journal of Biophotonics,2017,10:657-663.