动物营养学报 >

2019 , Vol. 31 >Issue 8: 3632 - 3642

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.024

反刍与草食动物营养 Ruminant and herbivore nutrition

过瘤胃不饱和脂肪对安格斯肉牛生长性能、血清指标及相关基因表达的影响

  • 杨致玲 ,
  • 杨国义 ,
  • 刘旗 ,
  • 赫磊 ,
  • 吴树峰 ,
  • 杨文强 ,
  • 张拴林 ,
  • 刘强 ,
  • 郭刚
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  • 1. 山西农业大学动物科技学院, 太谷 030801;
    2. 山西省林草局草原工作总站, 太原 030000
杨致玲(1965-),女,山西太谷人,高级实验师,学士,从事动物营养与饲料科学研究。E-mail:1652109648@qq.com

收稿日期: 2019-01-31

  网络出版日期: 2019-08-19

基金资助

山西省科技攻关项目（20140311022-2）；山西现代农业牛产业技术体系（18-05）

收起

Effects of Rumen Undegradable Unsaturated Fat on Growth Performance, Biochemical Indices and Related Gene Expression of Angus Beef

  • YANG Zhiling ,
  • YANG Guoyi ,
  • LIU Qi ,
  • HE Lei ,
  • WU Shufeng ,
  • YANG Wenqiang ,
  • ZHANG Shuanlin ,
  • LIU Qiang ,
  • GUO Gang
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  • 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    2. General Station of Grassland, Bureau of Forestry and Grassland of Shanxi Province, Taiyuan 030000, China

Received date: 2019-01-31

  Online published: 2019-08-19

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摘要

本试验旨在研究过瘤胃不饱和脂肪对安格斯肉牛生长性能、血清指标及相关基因表达的影响。选用24头平均体重（447.78±2.53）kg、15~17月龄安格斯去势公牛，随机分为4组，每组6头。1、2、3和4组分别饲喂含有0、2.0%、4.0%和6.0%过瘤胃不饱和脂肪的试验饲粮。试验期77 d，其中预试期10 d，正试期67 d，正试期分为前期（27 d）和后期（40 d）2个阶段。结果表明：1）试验前期，4组的平均日增重显著高于1和2组（P<0.05），1组的料重比显著高于4组（P<0.05）。试验前期、后期和全期，各组之间平均日采食量差异不显著（P>0.05）。2）试验前期和后期，2和3组的血清胰岛素（INS）含量显著高于1组（P<0.05），2、3和4组的血清胰高血糖素（GC）含量显著高于1组（P<0.05）。试验前期，3组的血清生长激素（GH）含量显著低于1组（P<0.05）；试验后期，2、3和4组的血清GH含量显著低于1组（P<0.05）。3）试验前期和后期，各组之间血清甘油三酯脂肪酶（ATGL）、肉碱棕榈酰转移酶-Ⅰ（CPT-Ⅰ）和乙酰辅酶A羧化酶（AACase）活性没有显著差异（P>0.05）。4）试验前期和后期，各组之间血清葡萄糖（GLU）、甘油三酯（TG）、白蛋白（ALB）和总胆固醇（TCHO）含量及谷丙转氨酶（ALT）活性均无显著差异（P>0.05）。试验前期，4组的血清总蛋白（TP）含量显著高于1组（P<0.05），2、3和4组的血清丙二醛（MDA）含量显著低于1组（P<0.05），2、3和4组的血清谷草转氨酶（AST）活性显著高于1组（P<0.05）；试验后期，3和4组的血清MDA含量显著低于1组（P<0.05），2和3组的血清AST活性显著高于1组（P<0.05）。5）4组的背最长肌中过氧化物酶体增殖物激活受体γ（PPARγ）的基因表达量显著高于1组（P<0.05）。由此可见，饲粮中添加过瘤胃不饱和脂肪对试验全期的平均日增重、平均采食量和料重比没有显著影响，对血清ATGL、CPT-Ⅰ和AACase的活性没有显著影响，提高了血清GC和INS含量，促进了背最长肌PPARγ的基因表达。综合以上结果，本试验条件下，饲粮过瘤胃不饱和脂肪适宜添加量为4.0%。

关键词： 过瘤胃脂肪; 生长性能; 血清指标; FASN; PPAR&gamma

本文引用格式

杨致玲 , 杨国义 , 刘旗 , 赫磊 , 吴树峰 , 杨文强 , 张拴林 , 刘强 , 郭刚 . 过瘤胃不饱和脂肪对安格斯肉牛生长性能、血清指标及相关基因表达的影响[J]. 动物营养学报, 2019 , 31(8) : 3632 -3642 . DOI: 10.3969/j.issn.1006-267x.2019.08.024

Abstract

The objective of the current study was to evaluate the effects of rumen undegradable unsaturated fat (RUF) on growth performance, biochemical indices and related gene expression of Angus beef. Twenty-four Angus steers[average body weight of (447.78±2.53) kg, 15 to 17 months of age] were randomly assigned to 4 groups with 6 beef in each group. Groups 1, 2, 3 and 4 were fed experimental diets containing 0, 2.0%, 4.0% and 6.0% RUF, respectively. The experiment lasted 77 days including a 10-day preliminary test period and a 67-day formal test period, and the formal test period was divided into two stages:early stage (27 days) and later stage (40 days). The results showed as follows:1) in early stage, the average daily gain of group 4 was significantly higher than that of groups 1 and 2 (P<0.05), the ratio of feed to gain of group 1 was significantly higher than that of group 4 (P<0.05). In early stage, later stage and whole stage, there was no significant difference on the average daily feed intake among all groups (P>0.05). 2) In early stage and later stage, the serum insulin (INS) content of groups 2 and 3 was significantly higher than that of group 1 (P<0.05), the serum glucagon (GC) content of groups 2, 3 and 4 was significantly higher than that of group 1 (P<0.05). In early stage, the serum growth hormone (GH) content of group 3 was significantly lower than that of group 1 (P<0.05); in later stage, the serum GH content of groups 2, 3 and 4 was significantly lower than that of group 1 (P<0.05). 3) In early stage and later stage, there were no significant differences on the activities of adipose triglyceride lipase (ATGL), carnitine palmitoyl transferase-Ⅰ (CPT-Ⅰ) and acetyl-coenzyme A carboxylase (AACase) in serum among all groups (P>0.05). 4) In early stage and later stage, there were no significant differences on the glucose (GLU), triglyceride (TG), albumin (ALB) and total cholesterol (TCHO) contents and alanine aminotransferase (ALT) activity in serum among all groups (P>0.05). In early stage, the serum total protein (TP) content of group 4 was significantly higher than that of group 1 (P<0.05), the serum malondialdehyde (MDA) content of groups 2, 3 and 4 was significantly lower than that of group 1 (P<0.05), the serum glutamic oxaloacetic transaminase (AST) activity of groups 2, 3 and 4 was significantly higher than that of group 1 (P<0.05); in later stage, the serum (MDA content of groups 3 and 4 was significantly lower than that of group 1 (P<0.05), the serum AST activity of groups 2 and 3 was significantly higher than that of group 1 (P<0.05). 5) The gene expression of peroxisome proliferator-activated receptor γ (PPARγ) in Longissimus dorsi of group 4 was significantly higher than that of group 1 (P<0.05). In conclusion, dietary RUF has no significant effects on the average daily gain, average daily feed intake and ratio of feed to gain of beef in whole stage, and has no significant effects on the activities of aATGL, CPT-Ⅰ and AACase in serum, but improves the contents of GC and INS in serum, promotes the gene expression of PPARγ in Longissimus dorsi. Overall consideration, under this experimental conditions, the optimum dietary RUF content is 4.0%.

Key words： rumen protected fat; growth performance; serum indices; FASN; PPARγ

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