饲料科学与技术 Feed science and technology

富硒胞外多糖对断奶仔猪生长、抗氧化功能、肠道形态结构和抗菌肽表达的影响

  • 路则庆 ,
  • 胡喻涵 ,
  • 黄向韵 ,
  • 张煜 ,
  • 王凤芹 ,
  • 汪以真
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  • 浙江大学饲料科学研究所, 生物饲料安全与污染防控国家工程实验室, 农业部华东动物营养与饲料重点实验室, 浙江省饲料与动物营养重点实验室, 杭州 310058
路则庆(1986-),男,山东泰安人,博士,动物营养与饲料科学专业。E-mail:zqlu2012@zju.edu.cn

收稿日期: 2019-01-31

  网络出版日期: 2019-08-19

基金资助

国家现代生猪产业技术体系岗位科学家项目(CARS-35);浙江省重点研发计划项目(2015C02022)

Effects of Selenium-Enrich Exopoly Saccharide on Growth Performance, Antioxidant Function, Intestinal Morphology and Antibacterial Peptide Expression of Weaned Piglets

  • LU Zeqing ,
  • HU Yuhan ,
  • HUANG Xiangyun ,
  • ZHANG Yu ,
  • WANG Fengqin ,
  • WANG Yizhen
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  • National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Ministry of Agriculture, Institute of Feed Science, Zhejiang University, Hangzhou 310058, China

Received date: 2019-01-31

  Online published: 2019-08-19

摘要

本试验旨在探究阴沟肠杆菌(Enterobacter cloacae)Z0206所产胞外多糖(EPS)和富硒胞外多糖(Se-EPS)对断奶仔猪生长性能、抗氧化功能、肠道形态结构和抗菌肽表达的影响。选择体重相近的28日龄"杜×长×大"三元杂交断奶仔猪150头,随机分为5组,每组3个重复,每个重复10头猪。对照(CON)组饲喂基础饲粮,亚硒酸钠(Na2SeO3)组饲喂基础饲粮+0.30 mg/kg Na2SeO3,Na2SeO3+黄芪多糖(APS)组饲喂基础饲粮+0.30 mg/kg Na2SeO3+560 mg/kg APS,Na2SeO3+EPS组饲喂基础饲粮+0.30 mg/kg Na2SeO3+560 mg/kg EPS,Se-EPS组饲喂基础饲粮+560 mg/kg Se-EPS。试验期39 d。结果表明:与Na2SeO3组相比,饲粮中添加Se-EPS显著提高了断奶仔猪的平均日增重(P<0.05),显著降低料重比(P<0.05),显著提高了血清总抗氧化能力(P<0.05),显著降低了血清丙二醛含量(P<0.05)。与对照组相比,饲粮中添加Na2SeO3+EPS、Se-EPS显著提高了断奶仔猪空肠的绒毛高度以及绒毛高度/隐窝深度(V/C)(P<0.05),饲粮中添加Se-EPS显著提高了十二指肠中猪β-防御素1(pBD-1)和空肠、回肠中猪β-防御素2(pBD-2)的mRNA相对表达量(P<0.05)。由此可见,饲粮中添加Se-EPS可提高断奶仔猪生长性能、抗氧化功能以及促进肠道内源抗菌肽的表达。

本文引用格式

路则庆 , 胡喻涵 , 黄向韵 , 张煜 , 王凤芹 , 汪以真 . 富硒胞外多糖对断奶仔猪生长、抗氧化功能、肠道形态结构和抗菌肽表达的影响[J]. 动物营养学报, 2019 , 31(8) : 3755 -3762 . DOI: 10.3969/j.issn.1006-267x.2019.08.037

Abstract

This experiment was conducted to investigate the effects exopoly saccharides (EPS) and selenium-enriched exopoly saccharides (Se-EPS) produced by Enterobacter cloacae Z0206 on growth performance, antioxidant function, intestinal morphology and antibacterial peptide expression of weaned piglets. A total of 150 piglets (Landrace×Yorkshire×Duroc) weaned at 28 days of age with similar body weight were randomly allocated into 5 groups with 3 replicates per group and 10 pigs per replicate. Pigs in the control (CON) group were fed a basal diet, pigs in the sodium selenite (Na2SeO3) group fed the basal diet+0.30 mg/kg Na2SeO3, pigs in the Na2SeO3+ astragalus polysaccharides (APS) group fed the basal diet+0.30 mg/kg Na2SeO3+560 mg/kg APS, pigs in the Na2SeO3+EPS group fed the basal diet+0.30 mg/kg Na2SeO3+560 mg/kg EPS, and pigs in the Se-EPS group fed the basal diet+560 mg/kg Se-EPS. The experiment lasted for 39 days. The results showed that compared with Na2SeO3 group, dietary supplemented with Se-EPS significantly increased the average daily gain of weaned piglets (P<0.05), significantly decreased the ratio of feed to gain (P<0.05), significantly increased the serum total antioxidant capacity (P<0.05), and significantly decreased the serum malondialdehyde content (P<0.05). Compared with the control group, dietary supplemented with Na2SeO3+EPS and Se-EPS significantly increased the villus height and villus height/crypt depth (V/C) in jejunum of weaned piglets (P<0.05), and dietary supplemented with Se-EPS significantly increased the mRNA relative expression of porcine β-defensin 1 (pBD-1) in duodenum and porcine β-defensin 2 (pBD-2) in jejunum and ileum (P<0.05). In conclusion, dietary supplemented with Se-EPS can improve the growth performance and antioxidant function, and promote intestinal endogenous antimicrobial peptide expression of weaned piglets.

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