补喂不同水平雌马酚对母绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响

王晨晨; 张文杰; 侯传祥; 李晓斌; 赵国栋; 杨开伦

doi:10.3969/j.issn.1006-267x.2019.08.042

动物营养学报 >

2019 , Vol. 31 >Issue 8: 3801 - 3809

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.042

饲料科学与技术 Feed science and technology

补喂不同水平雌马酚对母绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响

王晨晨 ,
张文杰 ,
侯传祥 ,
李晓斌 ,
赵国栋 ,
杨开伦

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新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052

王晨晨(1993-),女,新疆乌苏人,硕士研究生,研究方向为草食动物营养代谢。E-mail:2225927653@qq.com

收稿日期: 2019-01-21

网络出版日期: 2019-08-19

基金资助

新疆维吾尔自治区重点实验室开放课题“雌马酚高效转化细菌筛选及富含雌马酚牛乳生产的研究”（2016D03012）

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Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep

WANG Chenchen ,
ZHANG Wenjie ,
HOU Chuanxiang ,
LI Xiaobin ,
ZHAO Guodong ,
YANG Kailun

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Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2019-01-21

Online published: 2019-08-19

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摘要

本试验旨在研究补喂不同水平雌马酚对绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响。试验选取年龄为2岁、平均体重为（36.73±2.42）kg的健康、体况相近的哈萨克母羊20只，随机分为4组，分别为对照组、试验Ⅰ组、试验Ⅱ组、试验Ⅲ组，每组5只羊。各组试验羊在相同的饲养条件下饲养，在此基础上，试验Ⅰ组、试验Ⅱ组、试验Ⅲ组每日每只分别补喂10、25、50 mg雌马酚，进行为期14 d的饲养试验。结果表明：1）与对照组相比，每日每只母绵羊补喂50 mg雌马酚可以显著或极显著提高平均日增重、平均日采食量（P<0.05或P<0.01），显著降低料重比（P<0.05）。2）补喂雌马酚后血浆中雌马酚含量呈先上升后下降趋势，在2.0~3.0 h时达到峰值。3）试验Ⅰ组血浆中睾酮（T）水平显著或极显著高于对照组和试验Ⅲ组（P<0.05或P<0.01），试验Ⅱ组也显著高于对照组（P<0.05），但与试验Ⅰ组和试验Ⅲ组无显著差异（P>0.05）；试验Ⅰ组和试验Ⅱ组血浆中双氢睾酮（DHT）水平显著高于对照组和试验Ⅲ组（P<0.05）。4）血浆总抗氧化能力（T-AOC）与过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性均以试验Ⅱ组最高，丙二醛（MDA）含量以试验Ⅱ组最低，与对照组的差异均达到显著或极显著水平（P<0.05或P<0.01）。由此得出，在本试验条件下，每日给每只母绵羊补喂50 mg雌马酚可以显著提高绵羊的生长性能；补喂雌马酚后2.0~3.0 h，母绵羊血浆中雌马酚含量达到峰值，并且随着补喂剂量的提高，血浆中高含量雌马酚的跨峰时间（即维持血浆高含量雌马酚的持续时间）也延长。每日给每只母绵羊补喂10和25 mg雌马酚可显著提高血浆中T和DHT水平。每日给每只母绵羊补喂25 mg雌马酚可显著提高机体抗氧化能力，效果优于补喂10、50 mg时。。

关键词： 绵羊; 雌马酚; 生产性能; 生殖激素; 抗氧化能力

本文引用格式

王晨晨 , 张文杰 , 侯传祥 , 李晓斌 , 赵国栋 , 杨开伦 . 补喂不同水平雌马酚对母绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响[J]. 动物营养学报, 2019 , 31(8) : 3801 -3809 . DOI: 10.3969/j.issn.1006-267x.2019.08.042

Abstract

This experiment was conducted to investigate the effects of supplemented with different levels of equol on growth performance, plasma equol content and reproductive hormone levels and antioxidant capacity of female sheep. Twenty Kazakh female sheep with an average body weight of (36.73±2.42) kg, aged 2 years old, were randomly divided into 4 groups (5 sheep in each group), being control group, trial group Ⅰ, trial group Ⅱ and trial group Ⅲ. Each sheep in all group was fed under the same feeding condition, additionally, sheep in trial group Ⅰ, trial group Ⅱ and trial group Ⅲ were fed with 10, 25 and 50 mg equol at 08:00 every day per sheep, respectively. The supplementary feeding experiment lasted for 14 days. The results showed as follows:1) compared with control group, supplementation of 50 mg/(d·sheep) equol can significantly improve the average daily gain and average daily feed intake (P<0.05 or P<0.01), while reduce the feed/gain (P<0.05). 2) After different dosages of equol were supplementary fed, the plasma equol content tendency rising up at the beginning and declining in late, and it peaked around 2.0 to 3.0 h. 3) The plasma testosterone (T) level in trial group Ⅰ was significantly higher than that in control group (P<0.01) and trial group Ⅲ (P<0.05), and that in trial group Ⅱ was also significantly higher than that in control group (P<0.05), but there was no significant difference between trial group Ⅰ and trial group Ⅲ (P>0.05). The plasma dihydrotestosterone (DHT) level in trial group Ⅰ and trial group Ⅱ was significantly higher than that in control group and trial group Ⅲ (P<0.05). 4) The plasma total antioxidant capacity (T-AOC) and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in trial group Ⅱ had the highest values, while the malondialdehyde (MDA) content had the lowest value, and the differences in above indices were significant compared with control group (P<0.05 or P<0.01). Therefore, supplementation of 50 mg/(d·sheep) equol can significantly improve the growth performance of sheep; plasma equol content reaches its peak at 2.0 to 3.0 h after supplementary feeding equol, and with the supplemental dosage of equol increasing, the high equol content (i.e. duration time of high plasma equol content) prolongs in cross-peak time, too. Supplementation of 10 and 25 mg/(d·sheep) equol can significantly elevate the plasma T and DHT levels of female sheep. Supplementation of 25 mg/(d·sheep) equol can significantly enhance the antioxidant capacity of female sheep, and the effect is better than 10 and 50 mg/(d·sheep).

Key words： sheep; equol; growth performance; reproductive hormones; antioxidant capacity

参考文献

[1] 郑庆虎,周辉,陈祯祥,等.雌马酚的生物学作用及其在医学领域的研究进展[J].中国皮肤性病学杂志,2018(1):105-109.
[2] SETCHELL K D R,BROWN N M,LYDEKING-OLSEN E.The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones[J].The Journal of Nutrition,2002,132(12):3577-3584.

[3] SHUTT D A,COX R I.Steroid and phyto-oestrogen binding to sheep uterine receptors in vitro[J].Journal of Endocrinology,1972,52(2):299-310.

[4] SETCHELL K D R,CLERICI C,LEPHART E D,et al.S-equol,a potent ligand for estrogen receptor β,is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora[J].The American Journal of Clinical Nutrition,2005,81(5):1072-1079.

[5] LEGETTE L L,MARTIN B R,SHAHNAZARI M,et al.Supplemental dietary racemic equol has modest benefits to bone but has mild uterotropic activity in ovariectomized rats[J].The Journal of Nutrition,2009,139(10):1908-1913.

[6] ARORA A,NAIR M G,STRASBURG G M.Antioxidant activities of isoflavones and their biological metabolites in a liposomal system[J].Archives of Biochemistry and Biophysics,1998,356(2):133-141.

[7] 马得莹,单安山,杜娟.大豆黄酮对动物生产性能、免疫功能与抗氧化作用的影响[J].东北农业大学学报,2004,35(4):505-508.
[8] 林厦菁,蒋守群,林哲敏,等.大豆异黄酮和抗生素对文昌鸡生长性能、肉品质和血浆抗氧化指标的影响[J].华南农业大学学报,2018,39(1):1-6.
[9] 周玲玲,徐建雄.高活性大豆异黄酮发酵豆粕对断奶仔猪生长性能、抗氧化能力、肝功能指标的影响[J].饲料工业,2017,38(20):34-38.
[10] JÖEHLE W,LAMOND D R.Control of reproductive functions in domestic animals[M].London:Martinus Niihoff,1980,28N49.
[11] FORBES J M.The effects of sex hormones,pregnancy and lactation on digestion,metabolism and voluntary food intake[M]//MILLIGAN L P,GROVUM W L,DOBSON A.Control of digestion and metabolism in ruminants.New Jersey:Prentice-Hall,1986.
[12] 李海亮,邓颖,王欣,等.肠道微生物代谢产物——S-雌马酚与人类健康关系研究进展[J].中国微生态学杂志,2018,30(3):362-367.
[13] SETCHELL K D R,ZHAO X H,JHA P,et al.The pharmacokinetic behavior of the soy isoflavone metabolite S-(-)equol and its diastereoisomer R-(+)equol in healthy adults determined by using stable-isotope-labeled tracers[J].The American Journal of Clinical Nutrition,2009,90(4):1029-1037.

[14] 王洋,张玉梅.雌马酚人体代谢的研究进展[J].卫生研究,2010,39(6):787-789.
[15] CHOI E J.Evaluation of equol function on anti-or prooxidant status in vivo[J].Journal of Food Science,2009,74(4):64-71.
[16] WALSH K R,FAILLA M L.Transport and metabolism of equol by Caco-2 human intestinal cells[J].Journal of Agricultural and Food Chemistry,2009,57(18):8297-8302.

[17] SETCHELL K D R,ZHAO X H,SHOAF S E,et al.The pharmacokinetics of S-(-)equol administered as SE5-OH tablets to healthy postmenopausal women[J].The Journal of Nutrition,2009,139(11):2037-2043.

[18] SFAKIANOS J,COWARD L,KIRK M,et al.Intestinal uptake and biliary excretion of the isoflavone genistein in rats[J].The Journal of Nutrition,1997,127(7):1260-1268.

[19] MORITO K,HIROSE T,KINJO J,et al.Interaction of phytoestrogens with estrogen receptors α and β[J].Biological and Pharmaceutical Bulletin,2001,24(4):351-356.

[20] LUND T D,MUNSON D J,HALDY M E,et al.Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback[J].Biology of Reproduction,2004,70(4):1188-1195.

[21] 张晓娜,杨镒峰,常彤,等.雌激素、雄激素在卵泡发育中的作用及应用[J].特产研究,2017,39(3):50-54.
[22] 王镜岩.生物化学(上册)[M].3版.北京:高等教育出版社,2002:556-583.
[23] 吴彬,张勇,陈明亮,等.雌马酚对去卵巢后大鼠骨质疏松症的影响[J].第三军医大学学报,2015,37(3):256-260.
[24] 李琮,陈俊宏,刘亚伟,等.大豆黄酮、芒柄花素及其组合对奶牛产奶性能、血浆和乳中激素含量的影响[J].动物营养学报,2017,29(1):309-316.
[25] ADLERCREUTZ H,FOTSIS T,BANNWART C,et al.Determination of urinary lignans and phytoestrogen metabolites, potential antiestrogens and anticarcinogens, in urine of women on various habitual diets[J].Journal of Steroid Biochemistry,1986,25(5):791-797.

[26] BELL A W.Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation[J].Journal of Animal Science,1995,73(9):2804-2819.

[27] DIBNER J J,KITCHELL M L,ATWELL C A,et al.The effect of dietary ingredients and age on the microscopic structure of the gastrointestinal tract in poultry[J].The Journal of Applied Poultry Research,1996(5):70-77.
[28] KLADNA A,BERCZYNSKI P,KRUK I,et al.Studies on the antioxidant properties of some phytoestrogens[J].Luminescence,2016,31(6):1201-1206.

[29] 李琮,臧长江,刘亚伟,等.添喂两种植物雌激素对奶牛乳成分、血液生化指标和抗氧化性能的影响[J].中国畜牧兽医,2017(6):126-130.
[30] HODGSON J M,CROFT K D,PUDDEY I B,et al.Soybean isoflavonoids and their metabolic products inhibit in vitro lipoprotein oxidation in serum[J].The Journal of Nutritional Biochemistry,1996,7(12):664-669.

[31] MITCHELL J H,GARDNER P T,MCPHAIL D B,et al.Antioxidant efficacy of phytoestrogens in chemical and biological model systems[J].Archives of Biochemistry and Biophysics,1998,360(1):142-148.

[32] CHOI E J.evaluation of equol function on anti-or prooxidant status in vivo[J].Journal of Food Science,2009,74(2):H65-H71.
[33] 苟钟勇,蒋守群,郑春田,等.雌马酚对鸡巨噬细胞抗氧化保护和免疫调控作用的研究[C]//全国动物生理生化第七届全国代表大会暨第十三次学术交流会论文集.沈阳:中国畜牧兽医学会,2014:193.
[34] 林厦菁,蒋守群.雌马酚对鸡小肠上皮细胞氧化应激模型的影响[C]//全国动物生理生化第七届全国代表大会暨第十三次学术交流会论文集.沈阳:中国畜牧兽医学会,2014:184-185.

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