动物营养学报 >

2019 , Vol. 31 >Issue 8: 3810 - 3820

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.043

饲料科学与技术 Feed science and technology

犬源复合益生菌对脾气虚犬盲肠菌群的影响

  • 曹护群 ,
  • 贺濛初 ,
  • 舒迎霜 ,
  • 夏晓东 ,
  • 冯士彬 ,
  • 李玉 ,
  • 王希春 ,
  • 吴金节
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  • 安徽农业大学动物科技学院, 合肥 230036
曹护群(1994-),女,安徽芜湖人,硕士研究生,从事兽医临床诊疗技术研究。E-mail:a18365268564@163.com

收稿日期: 2019-01-29

  网络出版日期: 2019-08-19

基金资助

国家重点研发项目（2016YFD0501009）

收起

Effects of Canine-Derived Compound Probiotics on Cecal Microflora in Splenic Qi Asthenia Canines

  • CAO Huqun ,
  • HE Mengchu ,
  • SHU Yingshuang ,
  • XIA Xiaodong ,
  • FENG Shibin ,
  • LI Yu ,
  • WANG Xichun ,
  • WU Jinjie
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  • College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2019-01-29

  Online published: 2019-08-19

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摘要

本试验旨在通过高通量测序技术研究犬源复合益生菌对脾气虚犬盲肠菌群的影响。选取24只1岁龄比格犬，随机分成4组：对照组、脾气虚组、自然恢复组和益生菌治疗组，每组6只。其中，对照组饲喂基础饲粮；脾气虚组、自然恢复组和益生菌治疗组在饲喂基础饲粮的同时每天按照10 mL/kg BW的剂量灌喂番泻叶水煎液，连续灌喂7 d，建立脾气虚模型。在试验第7天，采集脾气虚组犬盲肠内容物；对照组和自然恢复组犬继续饲喂基础饲粮7 d，益生菌治疗组犬在饲喂基础饲粮的基础上每天按照1×109 CFU/只的剂量灌服犬源复合益生菌7 d。在试验第14天，随机从对照组、自然恢复组和益生菌治疗组各选4只犬采集盲肠内容物。利用Illumina HiSeq 2500平台及QⅡME测序软件对各组犬盲肠内容物进行生物信息学分析。结果显示：1）对照组与益生菌治疗组盲肠菌群的ACE指数、Chao1指数、Shannon指数均高于脾气虚组与自然恢复组，而Simpson指数则低于脾气虚组与自然恢复组。2）益生菌治疗组盲肠菌群中拟杆菌门、纲、目、科以及芽孢杆菌纲、属的相对丰度均显著高于对照组和脾气虚组（P<0.05），其他菌群在不同分类水平上的相对丰度均与对照组无显著差异（P>0.05）。综上所述，犬源复合益生菌不仅能够使番泻叶诱导的脾气虚犬盲肠菌群多样性恢复至正常犬水平，而且还能够提高盲肠菌群中有益菌拟杆菌、芽孢杆菌的占比，对脾气虚犬盲肠菌群结构有良好的调节作用。

关键词： 脾气虚犬; 犬源复合益生菌; 盲肠菌群; 多样性; 高通量测序

本文引用格式

曹护群 , 贺濛初 , 舒迎霜 , 夏晓东 , 冯士彬 , 李玉 , 王希春 , 吴金节 . 犬源复合益生菌对脾气虚犬盲肠菌群的影响[J]. 动物营养学报, 2019 , 31(8) : 3810 -3820 . DOI: 10.3969/j.issn.1006-267x.2019.08.043

Abstract

The objective of this study was to analyze the effects of canine-derived probiotics on cecal microflora in splenic qi asthenia canines using Illumina HiSeq sequencing technology. Twenty-four Beagle canines, aged 1-year-old, were randomly divided into 4 groups, which were control group, splenic qi asthenia group, natural recovery group and probiotic treatment group. The canines in control group were fed a basal diet; the canines in splenic qi asthenia group, natural recovery group and probiotics treatment group were fed the basal diet and were gavaged with 10 mL/(kg BW·d) senna decoction for 7 days to establish splenic qi asthenia model. On the day 7 of trial, the canines in splenic qi asthenia group were dissected for collecting cecal contents; the canines in control group and natural recovery group still continue to fed the basal diet for 7 days; the canines in probiotics treatment group still continue to fed the basal diet and were gavaged with 1×109 CFU/d of canine-derived compound probiotic per canine for 7 days. On the day 14 of trial, four canines of each group were randomly selected to collect the cecal contents from the control group, the natural recovery group and the probiotic treatment group. The bioinformatics analysis of cecal contents for canines of each group was performed using the Illumina HiSeq sequencing platform and QⅡME sequencing software. The results showed as follows:1) the ACE index, Chao1 index and Shannon index of the cecal microflora in the control group and the probiotics treatment group were higher than those in the splenic qi asthenia group and the natural recovery group, while the Simpson index was lower than that in the splenic qi asthenia group and the natural recovery group. 2) Bacteroides at different classification levels such as phylum, class, order and family, and bacillus at class and genus levels, their relative abundances in the probiotics treatment group were significantly higher than those in the control group and the splenic qi asthenia group (P<0.05), while the relative abundances of other microflora at different classification levels were not significantly different compared with the control group (P>0.05). In summary, canine-derived probiotics can not only restore the cecal microflora diversity in splenic qi asthenia canines to the level of normal canines, but also increase the proportions of beneficial bacteria such as bacteroides and bacillus, and it has a good regulatory effect on the cecal microflora structure of splenic qi asthenia canines.

Key words： splenic qi asthenia canine; canine-derived compound probiotics; cecal microflora; diversity; high-throughput sequencing

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