动物营养学报 >

2019 , Vol. 31 >Issue 8: 3872 - 3881

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.08.049

研究简报 Short communications

复合酶制剂对断奶仔猪生长性能、营养物质表观消化率、血清抗氧化指标及内源消化酶活性的影响

  • 史林鑫 ,
  • 乔鹏飞 ,
  • 龙沈飞 ,
  • 陆文清
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  • 中国农业大学动物科技学院农业部饲料工业中心, 动物营养学国家重点实验室, 北京 100193
史林鑫(1995-),男,内蒙古赤峰人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1369319242@qq.com

收稿日期: 2019-01-29

  网络出版日期: 2019-08-19

基金资助

国家自然科学基金项目（31772612）

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Effects of Complex Enzyme Preparation on Growth Performance, Apparent Digestibility of Nutrients, Serum Antioxidant Indexes and Endogenous Digestive Enzyme Activities of Weaned Piglets

  • SHI Linxin ,
  • QIAO Pengfei ,
  • LONG Shenfei ,
  • LU Wenqing
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  • State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2019-01-29

  Online published: 2019-08-19

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摘要

本试验旨在评价复合酶制剂对断奶仔猪生长性能、营养物质表观消化率、血清抗氧化指标和内源消化酶活性的影响。选用84头初始体重为（7.22±0.22）kg的28日龄杜×长×大断奶仔猪，按完全随机区组设计分为2个组，每组7个重复，每个重复6头猪（公母各占1/2）。对照组仔猪饲喂玉米-豆粕型基础饲粮，复合酶组仔猪饲喂在基础饲粮中添加1 000 mg/kg复合酶制剂（包含4 000 U/g纤维素酶、1 500 U/g α-淀粉酶、150 U/g β-葡聚糖酶和3 000 U/g中性蛋白酶）的试验饲粮。饲养期为35 d，分为前期（1~14 d）和后期（15~35 d）2个阶段。结果显示：与对照组相比，饲粮中添加复合酶制剂显著降低断奶仔猪全期的料重比（F/G）（P<0.05），并有降低前期（P=0.09）及后期F/G（P=0.05）的趋势；复合酶组断奶仔猪前期及后期的中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）和粗脂肪（EE）表观消化率显著或极显著提高（P<0.05或P<0.01），前期有机物（OM）（P=0.07）、粗蛋白质（CP）（P=0.09）和总能（GE）的表观消化率（P=0.08）以及后期CP（P=0.07）和GE的表观消化率（P=0.09）有提高的趋势。与对照组相比，在试验第14天，饲粮中添加复合酶制剂显著或极显著提高血清总抗氧化能力（T-AOC）和总超氧化物歧化酶（T-SOD）活性（P<0.05或P<0.01），显著降低血清丙二醛（MDA）含量（P<0.05），趋于增加血清谷胱甘肽过氧化物酶（GSH-Px）活性（P=0.06）；在试验第35天，饲粮中添加复合酶制剂显著提高血清GSH-Px活性（P<0.05），对血清其余抗氧化指标无显著影响（P>0.05）。此外，与对照组相比，饲粮中添加复合酶制剂显著增强了空肠和回肠黏膜蔗糖酶以及回肠黏膜乳糖酶和胰脏胰脂肪酶活性（P<0.05），趋于增加空肠黏膜麦芽糖酶活性（P=0.07）。综上所述，饲粮中添加1 000 mg/kg复合酶制剂能通过提高营养物质的消化率和内源消化酶活性以及增强血清抗氧化能力来改善断奶仔猪机体健康水平和生长性能。

关键词： 复合酶; 断奶仔猪; 生长性能; 营养物质消化率; 抗氧化能力; 消化酶活性

本文引用格式

史林鑫 , 乔鹏飞 , 龙沈飞 , 陆文清 . 复合酶制剂对断奶仔猪生长性能、营养物质表观消化率、血清抗氧化指标及内源消化酶活性的影响[J]. 动物营养学报, 2019 , 31(8) : 3872 -3881 . DOI: 10.3969/j.issn.1006-267x.2019.08.049

Abstract

This experiment was conducted to evaluate the effects of complex enzyme preparation on growth performance, apparent digestibility of nutrients, serum antioxidant indexes and endogenous digestive enzyme activities of weaned piglets. Eighty-four 28-day-old weaned piglets (Duroc×Landrace×Yorkshire) with an initial body weight of (7.22±0.22) kg were randomly allocated to one of two groups in a randomized complete block design. Each group contained 7 replicates and each replicate contained 6 piglets (3 males and 3 females). Piglets in control group were fed a corn-soybean meal basal diet, and piglets in complex enzyme group were fed an experimental diet which supplemented with 1 000 mg/kg complex enzyme preparation (containing 4 000 U/g cellulase, 1 500 U/g α-amylase, 150 U/g β-glucanase and 3 000 U/g neutral protease) based on the basal diet. The trail period was 35 d, which was divided into two phases:early period (1 to 14 d) and later period (15 to 35 d). The results showed that compared with the control group, dietary supplemented with compound enzyme preparation could significantly decrease the feed/gain (F/G) of overall period (P<0.05) and the F/G in early (P=0.09) and later periods (P=0.05) both had the potential to be decreased; the apparent digestibility of neutral detergent fiber (NDF), acid detergent fiber (ADF) and ether extract (EE) in early and later periods of complex enzyme group was significantly increased (P<0.05 or P<0.01), and there was a tendency to increase the apparent digestibility of organic matter (OM) (P=0.07), crude protein (CP) (P=0.09) and gross energy (GE) (P=0.08) in early stage and the apparent digestibility of CP (P=0.07) and GE (P=0.09) in later stage in complex enzyme group. Compared with the control group, dietary supplemented with compound enzyme preparation significantly increased serum total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activity (P<0.05 or P<0.01), significantly decreased serum malondialdehyde (MDA) content (P<0.05), and tended to increase serum glutathione peroxidase (GSH-Px) activity (P=0.06) on day 14; dietary supplemented with compound enzyme preparation significantly increased the serum GSH-Px activity (P<0.05), and had no significant effects on the other serum antioxidant indices on day 35 (P>0.05). In addition, compared with the control group, dietary supplemented with compound enzyme preparation significantly enhanced the activities of sucrase in both jejunal and ileal mucosa, lactase in ileal mucosa and pancrelipase in pancreas (P<0.05), and there was a tendency to increase the maltase activity in jejunal mucosa in complex enzyme group (P=0.07). In conclusion, dietary supplemented with 1 000 mg/kg complex enzyme preparation has the potential to improve physiological health and growth performance of weaned piglets by increasing the digestibility of nutrients and the activities of endogenous digestive enzymes and enhancing the serum antioxidant capacity.

Key words： complex enzyme; weaned piglets; growth performance; apparent digestibility of nutrients; antioxidant capacity; digestive enzyme activities

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