研究简报 Short communications

脂多糖刺激对断奶仔猪脾脏炎症信号通路关键基因表达的影响

  • 汪洋 ,
  • 李先根 ,
  • 刘玉兰 ,
  • 朱惠玲 ,
  • 王秀英
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  • 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
汪洋(1994-),男,湖北潜江人,硕士研究生,从事猪营养生理机能调控的研究。E-mail:609671541@qq.com

收稿日期: 2019-01-22

  网络出版日期: 2019-08-19

基金资助

湖北省教育厅优秀中青年科技创新团队项目(T201508)

Effects of Lipopolysaccharide Challenge on Key Genes Expression of Inflammatory Signaling Pathway in Spleen of Weaned Piglets

  • WANG Yang ,
  • LI Xiangen ,
  • LIU Yulan ,
  • ZHU Huiling ,
  • WANG Xiuying
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2019-01-22

  Online published: 2019-08-19

摘要

本试验旨在研究脂多糖(LPS)刺激后不同时间断奶仔猪脾脏Toll样受体4(TLR4)和核苷酸结合寡聚域受体(NOD)炎症信号通路关键基因表达的变化。选择42头体重为(7.1±0.9)kg的杜×长×大断奶仔猪,按注射LPS之前(0 h)和注射LPS后不同时间点(1、2、4、8、12、24 h)随机分为7个处理,每个处理6头猪。预饲14 d后,腹腔注射100 μg/kg BW的LPS,分别于注射LPS之前和注射LPS后不同时间点,将仔猪麻醉屠宰,取血液和脾脏样品,测定血细胞分类计数和脾脏炎症信号通路关键基因及炎症相关基因的mRNA表达量。结果表明:LPS刺激导致部分血细胞分类计数发生显著的变化(P<0.05);使TLR4和NOD炎症信号通路关键基因mRNA表达量显著上调(P<0.05),且在2~8 h达到峰值;也导致肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6、环氧合酶2、热休克蛋白70 mRNA表达量显著升高(P<0.05),且在1~4 h达到峰值。LPS刺激8~24 h后,这些炎症相关基因均逐渐恢复至正常水平或略低于正常水平。总体来看,LPS刺激激活了炎症信号通路,促进炎性细胞因子的表达,并随着时间的延长,这些基因呈现先上升、达到高峰后逐渐下降的变化。

本文引用格式

汪洋 , 李先根 , 刘玉兰 , 朱惠玲 , 王秀英 . 脂多糖刺激对断奶仔猪脾脏炎症信号通路关键基因表达的影响[J]. 动物营养学报, 2019 , 31(8) : 3882 -3890 . DOI: 10.3969/j.issn.1006-267x.2019.08.050

Abstract

The purpose of this study was to investigate the changes of key genes of Toll-like receptor 4 (TLR4) and nucleotide-bound oligomeric domain receptor (NOD) inflammation signaling pathway in spleen of weaned piglets at different time after lipopolysaccharide (LPS) challenge. Forty-two Duroc×Landrace×Yorkshine weaned piglets with a body weight of (7.1±0.9) kg were selected and randomly divided into 7 treatments (6 pigs per treatment). The piglets were injected with 100 μg/kg body weight LPS, and slaughtered at 0 h (before LPS challenge), 1, 2, 4, 8, 12 and 24 h (after LPS challenge). Blood and spleen samples were taken to determine the blood cell differential counts and the mRNA expressions of inflammatory related genes, respectively. The results showed as follows:LPS challenge led to significant changes in blood cell classification count (P<0.05). The mRNA expressions of key genes of TLR4 and NOD signaling pathway were increased after LPS challenge (P<0.05), and reached the peak at 2 to 8 h. The mRNA expressions of tumor necrosis factor-α, interleukin-1β, interleukin-6, cyclooxygenase 2 and heat shock protein 70 were also significantly increased after LPS challenge (P<0.05), and peaked at 1 to 4 h. At 8 to 24 h post injection, these genes gradually returned to normal level or slightly lower than normal level. Overall, LPS challenge activates the inflammatory signaling pathway and up-regulates the expression of inflammatory cytokines. During LPS stimulation, the expressions of these gene increase at the early time, and then decrease gradually after the peak.

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