研究简报 Short communications

紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备

  • 张虎 ,
  • 王庚南 ,
  • 王建平 ,
  • 马雨红 ,
  • 苏铭 ,
  • 刘聚祥
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  • 河北农业大学动物医学院, 保定 071000
张虎(1991-),男,河北衡水人,硕士研究生,从事基础兽医学研究。E-mail:1041395440@qq.com

收稿日期: 2019-01-29

  网络出版日期: 2019-08-19

基金资助

河北省自然基金青年基金项目(C2016204039);河北省高等学校科学技术研究项目(QN2018170);河北省蛋鸡体系(HBCT2018150210)

Isolation and Purification of Chicoric Acid from Echinacea purpurea Root and Its Molecularly Imprinted Polymer Preparation

  • ZHANG Hu ,
  • WANG Gengnan ,
  • WANG Jianping ,
  • MA Yuhong ,
  • SU Ming ,
  • LIU Juxiang
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  • College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China

Received date: 2019-01-29

  Online published: 2019-08-19

摘要

菊苣酸为天然药物紫锥菊中有效酚酸类化合物,本试验旨在研究紫锥菊根中菊苣酸提取纯化的新方法。试验采用超声辅助提取法对紫锥菊根粉末进行提取,所得粗提物再用葡聚糖凝胶LH-20为固定相,80%、60%及40%甲醇为流动相,进行反复提取,得到菊苣酸单体。再将制得的菊苣酸单体作为模版分子,α-甲基丙烯酸为功能单体,乙二醇二甲基烯酸酯为交联剂,制备菊苣酸分子印迹聚合物,并应用此分子印迹聚合物为固定相,对紫锥菊根粉进行富集纯化。结果显示,经葡聚糖凝胶LH-20提取分离后,高效液相色谱法测定菊苣酸含量为90.22%。紫锥菊根粉经分子印迹聚合物富集纯化后,可同时富集母核结构相同的菊苣酸和咖啡酸。由此可见,本方法可用于紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备。

本文引用格式

张虎 , 王庚南 , 王建平 , 马雨红 , 苏铭 , 刘聚祥 . 紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备[J]. 动物营养学报, 2019 , 31(8) : 3914 -3920 . DOI: 10.3969/j.issn.1006-267x.2019.08.054

Abstract

Chicoric acid is one active phenolic acid component of natural herbal medicine Echinacea purpurea, and this study set out to investigate the new isolation and purification method of chicoric acid from Echinacea purpurea root. The chicoric acid monomer was isolated and purified by the sephadex gel LH-20 column which eluted by 80%, 60%, 40% methanol after extracted by ultrasonic extraction from Echinacea purpurea root powder. For the preparation of molecularly imprinted polymer of chicoric acid, the purified chicoric acid monomer above was used as the template molecule with the 4-vinylpyridine and ethylene glycol dimethacrylate to be functional monomer and crosslinker, respectively, and the Echinacea purpurea root powder was enrichment purified by the molecularly imprinted polymer. The results showed that after sephadex gel LH-20 column separated and purified, the chicoric acid content was 90.22% by high performance liquid chromatography. After enrichment purified by the molecularly imprinted polymer of Echinacea purpurea root powder, the cichoric acid and caffeic acid could simultaneous enrichment which was with the same mother nucleus structure. In conclusion, this method can used for the isolation and purification of chicoric acid from echinacea purpurea root and its molecularly imprinted polymer preparation.

参考文献

[1] 闫晓慧,谈锋.3种松果菊属植物的鉴别、活性成分及生物技术研究进展[J].中草药,2006,37(2):300-303.
[2] CHIOU S Y,SUNG J M,HUANG P W,et al.Antioxidant,antidiabetic,and antihypertensive properties of Echinacea purpurea flower extract and caffeic acid derivatives using in vitro models[J].Journal of Medicinal Food,2017,20(2):171-179.  
[3] NYALAMBISA M,OYEMITAN I A,MATEWU R,et al.Volatile constituents and biological activities of the leaf and root of Echinacea species from South Africa[J].Saudi Pharmaceutical Journal,2017,25(3):381-386.  
[4] 姚兴东,聂园梅,DATTA-GUPTA N.紫锥花种属中酚类化合物的HPLC分析[J].分析科学学报,2006,22(2):199-201.
[5] JIANG L,LI W Z,WANG Y C,et al.Effects of cichoric acid extract from Echinacea purpurea on collagen-induced arthritis in rats[J].The American Journal of Chinese Medicine,2014,42(3):679-692.  
[6] ZHU D,WANG Y T,DU Q W,et al.Cichoric acid reverses insulin resistance and suppresses inflammatory responses in the glucosamine-induced HepG2 cells[J].Journal of Agriculture and Food Chemistry,2015,63(51):10903-10913.  
[7] ZHANG H L,DAI L H,WU Y H,et al.Evaluation of hepatocyteprotective and anti-hepatitis B virus properties of cichoric acid from Cichorium intybus leaves in cell culture[J].Biological and Pharmaceutical Bulletin,2014,37(7):1214-1220.  
[8] SCHLERNITZAUER A,OIRY C,HAMAD R,et al.Chicoric acid is an antioxidant molecule that stimulates AMP kinase pathway in L6 myotubes and extends lifespan in Caenorhabditis elegans[J].PLoS One,2013,8(11):e78788.
[9] KUBAN-JANKOWSKA A,SAHU K K,GORSKA M,et al.Chicoric acid binds to two sites and decreases the activity of the YopH bacterial virulence factor[J].Oncotarget,2016,7(3):2229-2238.
[10] TSAI Y L,CHIU C C,CHEN J Y F,et al.Cytotoxic effects of Echinacea purpurea flower extracts and cichoric acid on human colon cancer cells through induction of apoptosis[J].Journal of Ethnopharmacology,2012,143(3):914-919.  
[11] LIU Q,CHEN Y W,SHEN C,et al.Chicoric acid supplementation prevents systemic inflammation-induced memory impairment and amyloidogenesis via inhibition of NF-kB[J].The FASEB Journal,2017,31(4):1494-1507.  
[12] 赵咏梅,杨力.紫锥菊中菊苣酸喷雾提取工艺的研究[J].陕西农业科学,2018,64(10):32-35.
[13] 陈理.高速逆流色谱分离制备菊苣酸单体[J].中国中药杂志,2009,34(13):1732-1733.
[14] 谢春燕,徐新军,谢鸷生,等.快速制备液相色谱分离紫锥菊中咖啡酰基酒石酸、菊苣酸和松果菊苷[J].中药新药与临床药理,2012,23(1):90-94.
[15] WANG G N,ZHANG L,SONG Y P,et al.Application of molecularly imprinted polymer based matrix solid phase dispersion for determination of fluoroquinolones,tetracyclines and sulfonamides in meat[J].Journal of Chromatography B,2017,1065-1066:104-111.
[16] YANG K,WANG G N,LIU H Z,et al.Preparation of dual-template molecularly imprinted polymer coated stir bar based on computational simulation for detection of fluoroquinolones in meat[J].Journal of Chromatography B,2017,1046:65-72.
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