综述 Review

基于代谢组学的乳汁中代谢物研究进展

  • 付力立 ,
  • 江婧 ,
  • 陶金忠 ,
  • 曹随忠
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  • 1. 四川农业大学动物医学院, 成都 611130;
    2. 宁夏大学农学院, 银川 750021
付力立(1994-),男,贵州贵阳人,硕士研究生,从事动物生殖内分泌与动物细胞工程研究。E-mail:18188504126@163.com

收稿日期: 2019-02-25

  网络出版日期: 2019-09-17

基金资助

国家自然科学基金地区科学基金项目(31660731);四川农业大学学科建设双支计划(03571537)

Advances on Metabolites in Human and Animal Milks Based on Metabolomics

  • FU Lili ,
  • JIANG Jing ,
  • TAO Jinzhong ,
  • CAO Suizhong
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  • 1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2019-02-25

  Online published: 2019-09-17

摘要

乳汁是为新生哺乳动物的生长发育提供必需营养的最佳食物。除了为新生哺乳动物提供营养外,还含有大量小分子代谢物,这些代谢物在母体和新生哺乳动物之间形成了复杂的联系。在对乳汁的研究中应用代谢组学分析可以挖掘其中小分子物质的变化情况,结合生物信息学分析能够将关键代谢标志物呈现,并预测营养素等对乳汁代谢组影响的作用机制。本文主要总结了人类乳汁和奶牛乳汁分析中应用到代谢组学技术的文献,并结合其他关于乳汁或代谢组学的研究,阐述了乳汁中部分低丰度差异代谢物的变化及其影响因素,剖析了未来在乳汁分析中的潜在方向,以期为揭示乳汁代谢组的生理机制提供新思路。

本文引用格式

付力立 , 江婧 , 陶金忠 , 曹随忠 . 基于代谢组学的乳汁中代谢物研究进展[J]. 动物营养学报, 2019 , 31(9) : 4000 -4007 . DOI: 10.3969/j.issn.1006-267x.2019.09.011

Abstract

Breast milk is the best food to provide essential nutrients for the growth and development of newborn mammals. The breast milk can provide nutrition for newborn mammals, and also contain a large number of metabolites, which form a complex relationship between mother and newborn mammals. The application of metabonomic analysis in the research of breast milk can discover the changes of tiny molecular substances, and combined with bioinformatics analysis, key metabolic markers can be presented, as well the action mechanism of the nutrients on milk metabolism spectrum can be predicted. This paper summarized the literature on the application of metabonomics in human milk and bovine milk analysis, and combined with the other milk or metabolomics research, described the variation and effect factors of some low abundance differential metabolites in breast milk, and analyzed the potential research direction of breast milk in the future, in order to provide new ideas for revealing the physiological mechanism of milk metabolites.

参考文献

[1] VERARDO V,GÍMEZ-CARAVACA A M,ARRÁEZ-ROMÁN D,et al.Recent advances in phospholipids from colostrum,milk and dairy by-products[J].International Journal of Molecular Sciences,2017,18(1):173.
[2] SENDA A,FUKUDA K,ISHⅡ T,et al.Changes in the bovine whey proteome during the early lactation period[J].Animal Science Journal,2011,82(5):698-706.  
[3] BARDANZELLU F,FANOS V,STRIGINI F A L,et al.Human breast milk:exploring the linking ring among emerging components[J].Frontiers in Pediatrics,2018,6:215.
[4] QI Y X,ZHAO X W,HUANG D W,et al.Exploration of the relationship between intestinal colostrum or milk,and serum metabolites in neonatal calves by metabolomics analysis[J].Journal of Agricultural and Food Chemistry,2018,66(27):7200-7208.  
[5] BODE L,MCGUIRE M,RODRIGUEZ J M,et al.It's alive:microbes and cells in human milk and their potential benefits to mother and infant[J].Advances in Nutrition,2014,5(5):571-573.  
[6] OBERMAJER T,POGACIC T.Commentary:relationship between milk microbiota,bacterial load,macronutrients,and human cells during lactation[J].Frontiers in Microbiology,2016,7:492.
[7] BRIERE C E,MCGRATH J M,JENSEN T,et al.Breast milk stem cells:current science and implications for preterm infants[J].Advances in Neonatal Care,2016,16(6):410-419.  
[8] CESARE MARINCOLA F,DESSÌ A,CORBU S,et al.Clinical impact of human breast milk metabolomics[J].Clinica Chimica Acta,2015,451:103-106.
[9] BARDANZELLU F,FANOS V,REALI A."Omics" in human colostrum and mature milk:looking to old data with new eyes[J].Nutrients,2017,9(8):843.
[10] YANG M,CAO X Y,WU R N,et al.Comparative proteomic exploration of whey proteins in human and bovine colostrum and mature milk using iTRAQ-coupled LC-MS/MS[J].International Journal of Food Sciences and Nutrition,2017,68(6):671-681.  
[11] ZAMBRUNI M,VILLALOBOS A,SOMASUNDERAM A,et al.Maternal and pregnancy-related factors affecting human milk cytokines among Peruvian mothers bearing low-birth-weight neonates[J].Journal of Reproductive Immunology,2017,120:20-26.
[12] SPEVACEK A R,SMILOWITZ J T,CHIN E L,et al.Infant maturity at birth reveals minor differences in the maternal milk metabolome in the first month of lactation[J].The Journal of Nutrition,2015,145(8):1698-1708.  
[13] SUNDEKILDE U K,DOWNEY E,O'MAHONY J A,et al.The effect of gestational and lactational age on the human milk metabolome[J].Nutrients,2016,8(5):304.
[14] WU J F,DOMELLOF M,ZIVKOVIC A M,et al.NMR-based metabolite profiling of human milk:a pilot study of methods for investigating compositional changes during lactation[J].Biochemical and Biophysical Research Communications,2016,469(3):626-632.  
[15] ZHANG Z Y,ADELMAN A S,RAI D,et al.Amino acid profiles in term and preterm human milk through lactation:a systematic review[J].Nutrients,2013,5(12):4800-4821.  
[16] ANDREAS N J,HYDE M J,GOMEZ-ROMERO M,et al.Multiplatform characterization of dynamic changes in breast milk during lactation[J].Electrophoresis,2015,36(18):2269-2285.  
[17] CHUANG C K,LIN S P,LEE H C,et al.Free amino acids in full-term and pre-term human milk and infant formula[J].Journal of Pediatric Gastroenterology and Nutrition,2005,40(4):496-500.  
[18] JAKOBSEN L H,KONDRUP J,ZELLNER M,et al.Effect of a high protein meat diet on muscle and cognitive functions:a randomised controlled dietary intervention trial in healthy men[J].Clinical Nutrition,2011,30(3):303-311.  
[19] NEGRO M,GIARDINA S,MARZANI B,et al.Branched-chain amino acid supplementation does not enhance athletic performance but affects muscle recovery and the immune system[J].The Journal of Sports Medicine and Physical Fitness,2008,48(3):347-351.
[20] TAJIRI K,SHIMIZU Y.Branched-chain amino acids in liver diseases[J].World Journal of Gastroenterology,2013,19(43):7620-7629.  
[21] NAGATA C,NAKAMURA K,WADA K,et al.Branched-chain amino acid intake and the risk of diabetes in a Japanese community:the takayama study[J].American Journal of Epidemiology,2013,178(8):1226-1232.  
[22] SERVILLO L,GIOVANE A,CAUTELA D,et al.Where does Nε-trimethyllysine for the carnitine biosynthesis in mammals come from?[J].PLoS One,2014,9(1):e84589.
[23] STEPHENS F B,WALL B T,MARIMUTHU K,et al.Skeletal muscle carnitine loading increases energy expenditure,modulates fuel metabolism gene networks and prevents body fat accumulation in humans[J].Journal of Physiology,2013,591(18):4655-4666.  
[24] BODE L.Human milk oligosaccharides:every baby needs a sugar mama[J].Glycobiology,2012,22(9):1147-1162.  
[25] JANTSCHER-KRENN E,ZHEREBTSOV M,NISSAN C,et al.The human milk oligosaccharide disialyllacto-N-tetraose prevents necrotising enterocolitis in neonatal rats[J].Gut,2012,61(10):1417-1425.  
[26] VILLASEÑOR A,GARCIA-PEREZ I,GARCIA A,et al.Breast milk metabolome characterization in a single-phase extraction,multiplatform analytical approach[J].Analytical Chemistry,2014,86(16):8245-8252.  
[27] GABRIELLI O,ZAMPINI L,GALEAZZI T,et al.Preterm milk oligosaccharides during the first month of lactation[J].Pediatrics,2011,128(6):e1520-e1531.
[28] De LEOZ M L A,GAERLAN S C,STRUM J S,et al.Lacto-N-tetraose,fucosylation,and secretor status are highly variable in human milk oligosaccharides from women delivering preterm[J].Journal of Proteome Research,2012,11(9):4662-4672.  
[29] DELPLANQUE B,GIBSON R,KOLETZKO B,et al.Lipid quality in infant nutrition:current knowledge and future opportunities[J].Journal of Pediatric Gastroenterology and Nutrition,2015,61(1):8-17.
[30] SINANOGLOU V J,CAVOURAS D,BOUTSIKOU T,et al.Factors affecting human colostrum fatty acid profile:a case study[J].PLoS One,2017,12(4):e0175817.
[31] BALLARD O,MORROW A L.Human milk composition:nutrients and bioactive factors[J].Pediatric Clinics of North America,2013,60(1):49-74.  
[32] SALAMON S,CSAPÍ J.Composition of the mother's milk Ⅱ.Fat contents,fatty acid composition.A review[J].Acta Universitatis Sapientiae-Alimentaria,2009,2(2):196-234.
[33] AZULAY CHERTOK I R,HAILE Z T,EVENTOV-FRIEDMAN S,et al.Influence of gestational diabetes mellitus on fatty acid concentrations in human colostrum[J].Nutrition,2017,36:17-21.
[34] ÅKERSTEDT M,FORSBÄCK L,LARSEN T,et al.Natural variation in biomarkers indicating mastitis in healthy cows[J].Journal of Dairy Research,2011,78(1):88-96.  
[35] SUNDEKILDE U K,FREDERIKSEN P D,CLAUSEN M R,et al.Relationship between the metabolite profile and technological properties of bovine milk from two dairy breeds elucidated by NMR-based metabolomics[J].Journal of Agricultural and Food Chemistry,2011,59(13):7360-7367.  
[36] SUNDEKILDE U K,POULSEN N A,LARSEN L B,et al.Nuclear magnetic resonance metabonomics reveals strong association between milk metabolites and somatic cell count in bovine milk[J].Journal of Dairy Science,2013,96(1):290-299.  
[37] BOUDONCK K J,MITCHELL M W,WULFF J,et al.Characterization of the biochemical variability of bovine milk using metabolomics[J].Metabolomics,2009,5(4):375-386.  
[38] XU W,VERVOORT J,SACCENTI E,et al.Milk metabolomics data reveal the energy balance of individual dairy cows in early lactation[J].Scientific Reports,2018,8(1):15828.
[39] LU J,ANTUNES FERNANDES E,PÁEZ CANO A E,et al.Changes in milk proteome and metabolome associated with dry period length,energy balance,and lactation stage in postparturient dairy cows[J].Journal of Proteome Research,2013,12(7):3288-3296.  
[40] SUN H Z,WANG D M,WANG B,et al.Metabolomics of four biofluids from dairy cows:potential biomarkers for milk production and quality[J].Journal of Proteome Research,2015,14(2):1287-1298.  
[41] KLEIN M S,BUTTCHEREIT N,MIEMCZYK S P,et al.NMR metabolomic analysis of dairy cows reveals milk glycerophosphocholine to phosphocholine ratio as prognostic biomarker for risk of ketosis[J].Journal of Proteome Research,2012,11(2):1373-1381.  
[42] TIAN H,ZHENG N,WANG W Y,et al.Integrated metabolomics study of the milk of heat-stressed lactating dairy cows[J].Scientific Reports,2016,6:24208.
[43] TOMASSINI A,CURONE G,SOLÈ M,et al.NMR-based metabolomics to evaluate the milk composition from Friesian and autochthonous cows of Northern Italy at different lactation times[J].Natural Product Research,2019,33(8):1085-1091.  
[44] ILVES A,HARZIA H,LING K,et al.Alterations in milk and blood metabolomes during the first months of lactation in dairy cows[J].Journal of Dairy Science,2012,95(10):5788-5797.  
[45] MAHER A D,HAYES B,COCKS B,et al.Latent biochemical relationships in the blood-milk metabolic axis of dairy cows revealed by statistical integration of 1H NMR spectroscopic data[J].Journal of Proteome Research,2013,12(3):1428-1435.  
[46] KLEIN M S,ALMSTETTER M F,SCHLAMBERGER G,et al.Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation[J].Journal of Dairy Science,2010,93(4):1539-1550.  
[47] YANG Y X,ZHENG N,ZHAO X W,et al.Metabolomic biomarkers identify differences in milk produced by Holstein cows and other minor dairy animals[J].Journal of Proteomics,2016,136:174-182.
[48] ZHANG T,ZHANG R,ZHANG L,et al.Changes in the milk metabolome of the giant panda (Ailuropoda melanoleuca) with time after Birth-Three phases in early lactation and progressive individual differences[J].PLoS One,2015,10(12):e0143417.
[49] MURGIA A,SCANO P,CONTU M,et al.Characterization of donkey milk and metabolite profile comparison with human milk and formula milk[J].LWT,2016,74:427-433.
[50] PALMA M,HERNÁNDEZ-CASTELLANO L E,CASTRO N,et al.NMR-metabolomics profiling of mammary gland secretory tissue and milk serum in two goat breeds with different levels of tolerance to seasonal weight loss[J].Molecular BioSystems,2016,12(7):2094-2107.  
[51] CABONI P,MURGIA A,PORCU A,et al.Gas chromatography-mass spectrometry metabolomics of goat milk with different polymorphism at the αS1-casein genotype locus[J].Journal of Dairy Science,2016,99(8):6046-6051.  
[52] CABONI P,MURGIA A,PORCU A,et al.A metabolomics comparison between sheep's and goat's milk[J].Food Research International,2019,119:869-875.
[53] SCANO P,MURGIA A,PIRISI F M,et al.A gas chromatography-mass spectrometry-based metabolomic approach for the characterization of goat milk compared with cow milk[J].Journal of Dairy Science,2014,97(10):6057-6066.  
[54] SCANO P,CUSANO E,CABONI P,et al.NMR metabolite profiles of dairy:a review[J].International Dairy Journal,2019,90:56-67.
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