动物营养学报 >

2019 , Vol. 31 >Issue 9: 4099 - 4109

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.022

反刍与草食动物营养 Ruminant and herbivore nutrition

山羊妊娠前期和后期采食量限制对母体血清、羊水和尿囊液中氨基酸组成的影响

  • 李西林 ,
  • 李恒芝 ,
  • 贺志雄 ,
  • 谭支良 ,
  • 颜琼娴
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  • 1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 畜禽养殖污染控制与资源化技术国家工程实验室, 湖南省畜禽健康养殖工程技术研究中心, 农业部中南动物营养与饲料科学观测实验站, 动物营养生理与代谢过程湖南省重点实验室, 长沙 410125;
    2. 中国科学院大学, 北京 100049;
    3. 南京农业大学动物科学技术学院, 南京 210095;
    4. 湖南省畜禽安全生产协同创新中心, 长沙 410128;
    5. 湖南省植物功能成分利用协同创新中心, 长沙 410128
李西林(1993-),男,山东临清人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:lin172224350@163.com

收稿日期: 2019-03-06

  网络出版日期: 2019-09-17

基金资助

国家自然科学基金项目（31402105，31730092）；中国科学院亚热带农业生态研究所青年创新团队项目（2017QNCXTD_ZCS）

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Maternal Amino Acid Composition in Serum, Amniotic Fluid and Allantoic Fluid Affected by Nutrient Restriction of Goats during Early and Late Gestations

  • LI Xilin ,
  • LI Hengzhi ,
  • HE Zhixiong ,
  • TAN Zhiliang ,
  • YAN Qiongxian
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  • 1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Research Center of Livestock & Poultry Sciences, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Hunan Provincial Key Laboratory of Nutritional Physiology and Metabolic Process, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    4. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China;
    5. Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China

Received date: 2019-03-06

  Online published: 2019-09-17

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摘要

本试验旨在研究山羊妊娠前期和后期采食量限制对母体血清、羊水和尿囊液中氨基酸（AA）组成的影响。在妊娠前期（第26~65天）和后期（第96~135天），分别将12只年龄[（2.0±0.3）岁]、胎次（2胎）和体重相近的怀孕湘东黑山羊随机分配到对照组（n=6，自由采食）和采食量限制组（n=6，40%采食量限制），妊娠第65天和135天，检测母体血清、羊水和尿囊液中AA含量。结果显示：妊娠前期，与对照组相比，采食量限制组胎儿的体重、体长和胸围显著增加（P<0.05），血清中缬氨酸（Val）、天冬氨酸（Asp）、脯氨酸（Pro）、总必需氨基酸（EAA）和总非必需氨基酸（NEAA）含量显著降低（P<0.05），羊水中Val、酪氨酸（Tyr）和赖氨酸（Lys）含量显著降低（P<0.05），尿囊液中苏氨酸（Thr）、蛋氨酸（Met）、丝氨酸（Ser）和甘氨酸（Gly）含量显著增加（P<0.05）。妊娠后期，与对照组相比，采食量限制组的胎儿体重显著降低（P<0.05），血清中Lys、Asp、谷氨酸（Glu）和Pro含量显著降低（P<0.05），羊水中总EAA、总NEAA和各AA含量也显著降低（P<0.05），尿囊液中Glu含量显著降低，尿囊液中Pro含量显著增加（P<0.05）。由此可见，妊娠前期采食量限制会促进胎儿的生长，而妊娠后期采食量限制会抑制胎儿的生长，不同妊娠期采食量限制对母体血液及子宫内AA代谢的影响存在差异。

关键词： 氨基酸; 采食量限制; 胎儿生长; 羊水; 尿囊液; 山羊

本文引用格式

李西林 , 李恒芝 , 贺志雄 , 谭支良 , 颜琼娴 . 山羊妊娠前期和后期采食量限制对母体血清、羊水和尿囊液中氨基酸组成的影响[J]. 动物营养学报, 2019 , 31(9) : 4099 -4109 . DOI: 10.3969/j.issn.1006-267x.2019.09.022

Abstract

This experiment was conducted to investigate the effects of nutrient restriction of goats during early and late gestations on maternal amino acid (AA) composition in serum, amniotic fluid. In early (the 26th to 65th days) and late gestation (the 96th to 135th days), twelve pregnant Xiangdong black goats with similar age[(2.0±0.3) years], parity (2 parities) and body weight were randomly assigned to control group (n=6, ad libitum) and feed intake restricted group (n=6, 40% feed intake restriction). The content of AA in maternal serum, amniotic fluid and allantoic fluid were measured on the 65th and 135th days of pregnancy. The results showed as follows:in early gestation, compared with the control group, the body weight, body length and thoracic circumference of fetuses in feed intake restricted group were significantly increased (P<0.05), the contents of valine (Val), aspartic acid (Asp), proline (Pro), total essential amino acids (EAA) and total non-essential amino acids (NEAA) in serum were significantly decreased (P<0.05), the contents of Val, tyrosine (Tyr) and lysine (Lys) in amniotic fluid were significantly decreased (P<0.05), and the contents of threonine (Thr), methionine (Met), serine (Ser) and glycine (Gly) in allantoic fluid were significantly increased (P<0.05). In late gestation, compared with the control group, the body weight of fetuses in feed intake restricted group was significantly decreased (P<0.05), the contents of Lys, Asp, glutamic acid (Glu) and Pro in serum were significantly decreased (P<0.05), the contents of total EAA, total NEAA and each AA in amniotic fluid were significantly decreased (P<0.05), the content of Glu in allantoic fluid was significantly decreased (P<0.05), and the content of Pro in allantoic fluid was significantly increased (P<0.05). In summary, feed intake restriction during early gestation promotes fetal growth, while inhibits fetal growth during late gestation; feed intake restriction during early and late gestations probably induces some extent variation in AA metabolism of maternal blood and uterus.

Key words： amino acids; feed intake restriction; fetal growth; amniotic fluid; allantoic fluid; goats

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