动物营养学报 >

2019 , Vol. 31 >Issue 9: 4200 - 4207

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.033

实验方法 Experimental Methods

体外法研究添加不同比例啤酒花颗粒和氯化铈及其组合对瘤胃发酵的影响

  • 陈攀亮 ,
  • 李妍 ,
  • 曹玉凤 ,
  • 李秋凤 ,
  • 高艳霞 ,
  • 李建国
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  • 1. 河北农业大学动物科技学院, 保定 071001;
    2. 河北农业大学动物医学院, 保定 071001;
    3. 河北省牛羊胚胎工程技术研究中心, 保定 071001
陈攀亮(1993-),男,河北邯郸人,硕士研究生,研究方向为反刍动物营养与饲料科学。E-mail:2305206891@qq.com

收稿日期: 2019-03-07

  网络出版日期: 2019-09-17

基金资助

国家现代农业（奶牛）产业技术体系建设专项资金（CARS-36）；河北省科技计划项目（16226604D）；河北省农业产业技术体系奶牛创新团队（HBCT2018120203）

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In vitro Study on Effects of Different Proportions of Hops Pellets and Cerium Chloride and Its Combination on Rumen Fermentation

  • CHEN Panliang ,
  • LI Yan ,
  • CAO Yufeng ,
  • LI Qiufeng ,
  • GAO Yanxia ,
  • LI Jianguo
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  • 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China;
    2. College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China;
    3. Embryo Engineering and Technological Center of Cattle and Sheep of Hebei, Baoding 071001, China

Received date: 2019-03-07

  Online published: 2019-09-17

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摘要

本试验旨在通过体外法研究添加不同比例啤酒花颗粒和氯化铈及其组合对瘤胃发酵的影响。选用高产奶牛的全混合日粮（TMR）和瘤胃液分别作为发酵底物和发酵菌群。采用4×4两因子试验设计，啤酒花颗粒分别设空白（0 mg/L）、低（L，3.34 mg/L）、中（M，6.67 mg/L）、高（H，10.00 mg/L）4个浓度，氯化铈分别设空白（0 mg/L）、低（l，1.33 mg/L）、中（m，1.60 mg/L）、高（h，1.87 mg/L）4个浓度，以满足发酵底物干物质中啤酒花颗粒的含量分别为0、0.5、1.0、1.5 g/㎏，氯化铈的含量分别为0、0.20、0.24、0.28 g/㎏。按照啤酒花颗粒与氯化铈的不同组合，共分为16个组，分别为C（空白对照）、l、m、h、L、Ll、Lm、Lh、M、Ml、Mm、Mh、H、Hl、Hm、Hh组，每组5个重复。使用美国ANKOM RFS气体测量系统厌氧发酵24 h，测定发酵结束后的pH、氨态氮（NH3-N）浓度、微生物蛋白（MCP）产量、干物质消化率（DMD）、产气量（GP）及乙酸、丙酸、总挥发性脂肪酸（TVFA）浓度等发酵指标。结果表明：1）单独添加啤酒花颗粒的组别中，L、M组的NH3-N浓度较C组显著降低（P<0.05），M组的MCP产量较C组极显著提高（P<0.01）。2）单独添加氯化铈的组别中，l、m、h组MCP产量较C组均显著或极显著提高（P<0.05或P<0.01），l组DMD、GP及乙酸、丙酸、总挥发性脂肪酸（TVFA）浓度较C组显著提高（P<0.05）。3）啤酒花颗粒与氯化铈组合对NH3-N、乙酸、丙酸、TVFA浓度和MCP产量的交互作用显著（P<0.05）；除Hh、Lh组外，各组NH3-N浓度较C组均极显著降低（P<0.01），其中Hl、Ll组较低；除Lh组外，各组MCP产量均显著或极显著增加（P<0.05或P<0.01），其中Lm、Ll、Ml组较高；Mh、Lh、Ll组TVFA浓度较C组显著提高（P<0.05）。综合分析各项指标，M、l、Ll组分别为啤酒花颗粒、氯化铈单独与组合添加组别中的最优组别，其瘤胃发酵性能提升效果依次为Ll组 > l组 > M组。

关键词： 啤酒花颗粒; 氯化铈; 体外法; 瘤胃发酵

本文引用格式

陈攀亮 , 李妍 , 曹玉凤 , 李秋凤 , 高艳霞 , 李建国 . 体外法研究添加不同比例啤酒花颗粒和氯化铈及其组合对瘤胃发酵的影响[J]. 动物营养学报, 2019 , 31(9) : 4200 -4207 . DOI: 10.3969/j.issn.1006-267x.2019.09.033

Abstract

The purpose of this experiment was to investigate the effects of different proportions of hops pellets and cerium chloride and its combination on rumen fermentation through a in vitro study. The total mixed ration (TMR) and rumen fluid of high-yield dairy cows were used as fermentation substrate and fermentation microflora. A 4×4 two-factor experiment design was adopted, the hops pellets were set 4 concentrations of blank (0 mg/L), low (L, 3.34 mg/L), middle (M, 6.67 mg/L) and high (H, 10.00 mg/L), respectively; the cerium chloride were set 4 concentrations of blank (0 mg/L), low (l, 1.33 mg/L), middle (m, 1.60 mg/L) and high (h, 1.87 mg/L), respectively, to meet the hops pellets contents of dry matter fermentation substrate were 0, 0.5, 1.0 and 1.5 g/kg, respectively; and the cerium chloride contents were 0, 0.20, 0.24 and 0.28 g/kg, respectively. According to different combinations of hops pellets and cerium chloride, 16 groups (5 replicates per group) were divided into C (blank control), l, m, h, L, Ll, Lm, Lh, M, Ml, Mm, Mh,H, Hl, Hm and Hh. Anaerobic fermentation was carried out for 24 h using the American ANKOM RFS gas measurement system, and the pH, ammonia nitrogen (NH3-N) concentration, microbial crude protein (MCP) yield, dry matter digestibility (DMD), gas production (GP) and the concentrations of acetic acid, propionic acid and total volatile fat acids (TVFA) were detected when fermentation stopped. The results showed as follows:1) among the groups adding hops pellets alone, the NH3-N concentration in groups L and M were significantly decreased than that in group C (P<0.05), the MCP yield in group M was significantly increased than that in group C (P<0.05). 2) Among the groups adding cerium chloride alone, the MCP yield in groups l, m and h was significantly increased than that in group C (P<0.05 or P<0.01), the DMD, GP and concentrations of acetic acid, propionic acid and TVFA in group l were significantly increased than those in group C (P<0.05). 3) The combination of hops pellets and cerium chloride had significant interactive effects on the NH3-N, acetic acid, propionic acid and TVFA concentrations and MCP yield (P<0.05). The NH3-N concentration in all groups except groups Hh and Lh was significantly decreased that in group C (P<0.01), and that in groups Hl and Ll was lower; the MCP yield in all groups except group Lh was significantly increased that in group C (P<0.05 or (P<0.01), and that in groups Lm, Ll, Ml was higher; and the TVFA concentration in groups Mh, Lh and Ll was significantly increased that in group C (P<0.05). According to the comprehensive analysis of each index, groups M, l and Ll are the optimal group among hops pellets and cerium chloride alone and its combination groups, the rumen fermentation performance effect can be improved in the order of group Ll > group l > group M.

Key words： hops pellets; cerium chloride; in vitro study; rumen fermentation

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