动物营养学报 >

2019 , Vol. 31 >Issue 9: 4218 - 4225

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.035

饲料科学与技术 Feed science and technology

壳聚糖对泌乳中期奶牛花生四烯酸免疫调节途径的影响

  • 李倜宇 ,
  • 闫素梅 ,
  • 史彬林 ,
  • 张鹏飞
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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
李倜宇(1987-),男,内蒙古锡林郭勒人,博士研究生,研究方向为动物环境与营养。E-mail:litiyu2050@163.com

收稿日期: 2019-02-21

  网络出版日期: 2019-09-17

基金资助

国家自然科学基金项目（31672463）

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Effects of Chitosan on Arachidonic Acid Immune Regulating Pathway in Mid-Lactating Dairy Cows

  • LI Tiyu ,
  • YAN Sumei ,
  • SHI Binlin ,
  • ZHANG Pengfei
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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2019-02-21

  Online published: 2019-09-17

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摘要

本试验旨在研究壳聚糖对泌乳中期奶牛花生四烯酸（AA）免疫调节途径的影响。将40头泌乳中期荷斯坦奶牛依照产奶量、泌乳期和胎次相一致的原则，随机分为5个组，每组8头牛。5组奶牛饲粮中壳聚糖添加水平分别为0（对照组）、500、1 000、1 500和2 000 mg/kg。试验期为60 d，分为试验前期（第1~30天）和后期（第31~60天）2个阶段。试验期间奶牛自由采食和饮水。结果显示：随着壳聚糖添加水平的升高，试验后期（第60天）血清中AA和白三烯B4（LTB4）浓度、5-脂氧化酶（5-LOX）活性及外周血淋巴细胞中胞浆型磷脂酶A2（cPLA2）和5-LOX基因表达量呈显著的一次线性升高效应（P<0.05），前列腺素E2（PGE2）和LTB4浓度、cPLA2和环氧合酶-2（COX-2）活性及外周血淋巴细胞中COX-2和5-LOX基因表达量呈显著的二次曲线升高效应（P<0.05）；试验前期（第30天）血清中cPLA2基因表达量呈极显著的一次线性和二次曲线升高效应（P<0.01），外周血淋巴细胞中5-LOX基因表达量呈显著的二次曲线升高效应（P<0.05）；上述指标均在1 500 mg/kg壳聚糖添加组表现为最佳。结果提示，壳聚糖可以通过增加cPLA2、COX-2和5-LOX基因的表达量及其活性，促进免疫细胞对AA代谢途径中主要介质AA、PGE2和LTB4的释放，进而影响泌乳中期奶牛的免疫功能，以1 500 mg/kg壳聚糖的免疫调节效果最佳。

关键词： 壳聚糖; 奶牛; 花生四烯酸; 免疫调节

本文引用格式

李倜宇 , 闫素梅 , 史彬林 , 张鹏飞 . 壳聚糖对泌乳中期奶牛花生四烯酸免疫调节途径的影响[J]. 动物营养学报, 2019 , 31(9) : 4218 -4225 . DOI: 10.3969/j.issn.1006-267x.2019.09.035

Abstract

This experiment was conducted to study the effects of chitosan on arachidonic acid immune regulating pathway in mid-lactating dairy cows. A total of forty healthy mid-lactating Holstein cows with similar milk yield, days in lactation and parity were randomly divided into five groups with eight cows per group. The five experimental diets contained 0 (control group), 500, 1 000, 1 500 and 2 000 mg/kg chitosan, respectively. Feeding trial lasted for 60 days, and was divided two phases:prophase (day 1 to 30) and anaphase (day 31 to 60). Cows had free access to feed and water during the feeding trial. The results showed as follows:with the of chitosan supplemental level increasing, the concentrations of arachidonic acid (AA) and leukotriene B4 (LTB4), the activity of 5-lipoxygenase (5-LOX) in serum and the gene expression levels of cytosolic phospholipase A2 (cPLA2) and 5-LOX in peripheral blood lymphocytes (PBLs) showed significant linear increasing effects on anaphase (day 60) (P<0.05). The concentrations of prostaglandin E2 (PGE2) and LTB4, the activities of cPLA2 and cyclooxygenase-2 (COX-2) in serum and the gene expression levels of COX-2 and 5-LOX in PBLs exhibited significant quadratic increasing effects on anaphase (day 60) (P<0.05). The gene expression level of cPLA2 in PBLs showed extremely significant linear and quadratic increasing effects on prophase (day 30) (P<0.01). The gene expression level of 5-LOX in PBLs exhibited significant quadratic increasing effects on prophase (day 30) (P<0.05). When the chitosan supplemental level was 1 500 mg/kg, all above indices got the best performance. The results indicate that chitosan can affect the immune function of mid-lactating dairy cows by increasing the gene expression levels of cPLA2, COX-2, and 5-LOX, improving the activities of cPLA2, COX-2 and 5-LOX, stimulating the releasing of main factors in AA metabolic pathway such as AA, PGE2 and LTB4 from immunological cells, and the dosage of 1 500 mg/kg chitosan has the best immunoregulatory effect.

Key words： chitosan; dairy cows; arachidonic acid; immune regulating

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