补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响

陆江; 朱道仙; 卢鹏飞; 张东豪; 刘静; 傅宏庆

doi:10.3969/j.issn.1006-267x.2019.09.038

动物营养学报 >

2019 , Vol. 31 >Issue 9: 4242 - 4250

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.038

饲料科学与技术 Feed science and technology

补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响

陆江 ,
朱道仙 ,
卢鹏飞 ,
张东豪 ,
刘静 ,
傅宏庆

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1. 江苏农牧科技职业学院宠物科技学院, 泰州 225300;
2. 江苏农牧科技职业学院动物医学院, 泰州 225300;
3. 江苏雅博动物健康科技有限责任公司, 泰州 225300

陆江(1982-),男,江苏新沂人,讲师,硕士,主要从事动物临床营养及代谢病研究。E-mail:vetlj@163.com

收稿日期: 2019-03-18

网络出版日期: 2019-09-17

基金资助

江苏省大学生创新创业训练计划项目（201812806015Y）；江苏农牧科技职业学院横向课题配套项目（NSFPT201845，NSFPT201842）

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Effects of Supplementary Feeding Complex-Probiotic-Preparation on Growth Performance, Intestinal Motility and Intestinal Barrier Function of Puppies

LU Jiang ,
ZHU Daoxian ,
LU Pengfei ,
ZHANG Donghao ,
LIU Jing ,
FU Hongqing

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1. Department of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
2. Department of Animal Medicine, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
3. Abram(Jiangsu) Animal Heath Co., Ltd., Taizhou 225300, China

Received date: 2019-03-18

Online published: 2019-09-17

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摘要

本试验旨在研究补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响。选取40只6周龄左右的贵宾犬，随机分为5组，每组2个重复，每个重复4只犬。其中，试验A组幼犬饲喂基础饲粮+0.2 g/（kg BW·d）复合益生菌制剂，试验B组幼犬饲喂基础饲粮+0.6 g/（kg BW·d）复合益生菌制剂，试验C组幼犬饲喂基础饲粮+1.0 g/（kg BW·d）复合益生菌制剂，阴性对照组幼犬饲喂基础饲粮，对比对照组幼犬饲喂基础饲粮+1.0 g/（kg BW·d）宠儿香康源益生菌，进行为期30 d的饲养试验。试验结束后取样，用酶联免疫吸附测定（ELISA）法检测血清胃肠激素含量和肠道屏障功能指标，免疫比浊法检测血清炎性细胞因子含量，实时荧光定量PCR（RT-qPCR）法检测空肠黏膜中相关基因mRNA的相对表达量。结果显示：1）随着复合益生菌制剂补喂剂量的增加，终末体重和平均日增重增加，腹泻率和便秘率降低，其中试验C组平均日增重显著高于其他各组（P<0.05），腹泻率和便秘率显著低于阴性对照组（P<0.05）。2）随着复合益生菌制剂补喂剂量的增加，血清中胃动素（MTL）、白细胞介素-10（IL-10）含量呈升高趋势，血管活性肠肽（VIP）、D-乳酸、内毒素（LPS）、肿瘤坏死因子-α（TNF-α）含量与二胺氧化酶（DAO）活性呈降低趋势，其中试验C组与阴性对照比较差异显著（P<0.05），与对比对照组相比差异不显著（P>0.05）。3）随着复合益生菌制剂补喂剂量的增加，回肠黏膜闭合蛋白（occludin）、闭锁小带蛋白-1（ZO-1）mRNA的相对表达量呈上调趋势，肌球蛋白轻链激酶（MLCK）mRNA的相对表达量呈下调趋势，其中试验C组与阴性对照组差异显著（P<0.05），与对比对照组差异不显著（P>0.05），且ZO-1 mRNA的相对表达量显著高于试验A组和试验B组（P<0.05）。以上结果表明，补喂复合益生菌制剂可增强幼犬胃肠动力和肠道屏障功能，缓解炎症应激，改善生长性能，以每天补喂1.0 g/kg BW效果最佳。

关键词： 复合益生菌制剂; 幼犬; 生长性能; 肠道动力; 肠道屏障功能

本文引用格式

陆江 , 朱道仙 , 卢鹏飞 , 张东豪 , 刘静 , 傅宏庆 . 补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响[J]. 动物营养学报, 2019 , 31(9) : 4242 -4250 . DOI: 10.3969/j.issn.1006-267x.2019.09.038

Abstract

The purpose of this experiment was to study the effects of supplementary feeding complex-probiotic-preparation on growth performance, intestinal motility and intestinal barrier function of puppies. Forty poodles at the age about 6 weeks were randomly divided into five groups with 2 replicates in each group and 4 poodles in each replicate. Among them, poodles in test group A were fed a basal diet+0.2 g/(kg BW·d) complex-probiotic-preparation; poodles in test group B were fed the basal diet+0.6 g/(kg BW·d) complex-probiotic-preparation; poodles in test group C were fed the basal diet+1.0 g/(kg BW·d) complex-probiotic-preparation; poodles in negative control group were fed the basal diet; and poodles in contrast control group were fed the basal diet+1.0 g/(kg BW·d) G-pet probiotic powder. The experiment lasted for 30 days. After experiment to sampling, the contents of gastrointestinal hormones and intestinal barrier function indicators in serum were detected by enzyme-linked immunosorbent assay (ELISA) method, serum inflammatory factor contents were detected by immunoturbidimetry, and mRNA relative expression levels of related genes in jejunum mucosa were detected by quantitative real-time fluorescence PCR (RT-qPCR). The results showed as follows:1) with the increase of feeding dosage of complex-probiotic-preparation, the final weight and average daily gain were increased, and the diarrhea rate and constipation rate were decreased. The average daily gain of test group C was significantly higher than that of other groups (P<0.05), and the diarrhea rate and constipation rate were significantly lower than those of negative control group (P<0.05). 2) With the increase of feeding dosage of complex-probiotic-preparation, the contents of motilin (MTL) and interleukin-10 (IL-10) in serum showed an increase trend, while the contents of asoactive intestinal peptide (VIP), D-lactic acid, lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α) and the activity of diamine oxidase (DAO) in serum showed a decrease trend. Significant differences in above indices were found between test group C and negative control group (P<0.05), but no significant differences were found between test group C and contrast control group (P>0.05). 3) With the increase of feeding dosage of complex-probiotic-preparation, the mRNA relative expression levels of occludin and zonula occluden-1 (ZO-1) in ileal mucosa were up-regulated, while the mRNA relative expression level of myosin light-chain kinase (MLCK) was down-regulated. Significant differences in above indices were found between test group C and negative control group (P<0.05), but no significant differences were found between test group C and contrast control group (P>0.05). The mRNA relative expression level of ZO-1 in test group C was significantly higher than that of test groups A and B (P<0.05). In summary, supplementary feeding complex-probiotic-preparation can enhance the gastrointestinal motility and intestinal barrier function, alleviate the inflammatory stress and improve the growth performance of puppies, and the best supplementary feeding dosage is 1.0 g/kg BW per day.

Key words： complex-probiotic-preparation; puppy; growth performance; intestinal motility; intestinal barrier function

参考文献

[1] 陈丽玲,陈丽雄.Beagle幼犬腹泻的综合防治[J].中国畜牧兽医,2006,33(11):90-91.
[2] SALISU S S,NURA S,SHAAIBU A S,et al.Potentials of probiotics as alternative therapy in combating bacterial diseases:a review[J].British Journal of Applied Science and Technology,2014,4(9):1392-1410.

[3] MAKIOKA Y,TSUKAHARA T,IJICHI T,et al.Oral supplementation of Bifidobacterium longum strain BR-108 alters cecal microbiota by stimulating gut immune system in mice irrespectively of viability[J].Bioscience,Biotechnology,and Biochemistry,2018,82(7):1180-1187.

[4] 曹煜成,李卓佳,冯娟,等.地衣芽孢杆菌胞外产物消化活性的研究[J].热带海洋学报,2005,24(6):6-12.
[5] 王运东,张俊秀,韩真.微生态制剂干预肠易激综合征患者疗效meta分析[J].中国微生态学杂志,2011,23(6):543-545.
[6] CHIANG M L,CHEN H C,CHEN K N,et al.Optimizing production of two potential probiotic Lactobacilli strains isolated from piglet feces as feed additives for weaned piglets[J].Asian-Australasian Journal of Animal Science,2015,28(8):1163-1170.

[7] 吴德华,杜莉,徐汉坤,等.纳豆芽孢杆菌对仔犬日增重和肠道菌群的影响[J].家畜生态学报,2009,30(3):64-67.
[8] 燕珍寿,许琴,周宇翔.添加益生菌对仔犬生长性能影响的研究[J].中国畜牧兽医文摘,2014,30(5):200,201.
[9] 赵平,董蕾,兰康,等.多种胃肠激素在消化间期移行性复合运动中作用的研究[J].中华消化杂志,2005,25(2):95-97.
[10] CARLINI V P,GHERSI M,SCHIÖTH H B,et al.Ghrelin and memory:differential effects on acquisition and retrieval[J].Peptides,2010,31(6):1190-1193.

[11] 牛亭惠,吴国泰,陈振鹤,等.幼龄厌食模型大鼠胃肠激素变化规律研究进展[J].甘肃中医学院学报,2017,34(1):82-85.
[12] DOCKRAY G,DIMALINE R,VARRO A.Gastrin:old hormone,new functions[J].Pflügers Archiv,2005,449(4):344-355.

[13] 莫伟彬,杨衍滔,郭艳菊,等.益生菌干预对运动大鼠胃肠激素与AQP4表达的影响[J].中国实验动物学报,2018,26(4):411-417.
[14] BAYGUINOV O,HAGEN B,SANDERS K M.Substance P modulates localized calcium transients and membrane current responses in murine colonic myocytes[J].British Journal of Pharmacology,2003,138(7):1233-1243.

[15] 赵力芳.双歧杆菌三联活菌胶囊联合思密达对儿童腹泻血浆VIP、NPY及肠黏膜5-HT的影响[J].中国微生态学杂志,2015,27(8):917-920.
[16] FUKUDOME I,KOBAYASHI M,DABANAKA K,et al.Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats[J].Medical Molecular Morphology,2014,47(2):100-107.

[17] 朱锦龙,方兴亮.急性胰腺炎患者早期血浆D-乳酸和内毒素水平与病情严重度评估系统的相关性研究[J].中华危重症医学杂志(电子版),2013,6(5):41-43.
[18] 胡长霞.益生菌对轮状病毒性腹泻小鼠肠道屏障的影响及机制的探讨[D].硕士学位论文.武汉:华中科技大学,2013.
[19] AL-SADI R,YE D M,SAID H M,et al.Cellular and molecular mechanism of interleukin-1β modulation of Caco-2 intestinal epithelial tight junction barrier[J].Journal of Cellular and Molecular Medicine,2011,15(4):970-982.

[20] FORSYTH C B,BANAN A,FARHADI A,et al.Regulation of oxidant-induced intestinal permeability by metalloprotease-dependent epidermal growth factor receptor signaling[J].Journal of Pharmacology and Experimental Therapeutics,2007,321(1):84-97.

[21] STURM A,RILLING K,BAUMGART D C,et al. Escherichia coli Nissle 1917 distinctively modulates T-cell cycling and expansion via Toll-like receptor 2 signaling[J].Infection and Immunity,2005,73(3):1452-1465.

[22] CUSTODERO C,MANKOWSKI R T,LEE S A,et al.Evidence-based nutritional and pharmacological interventions targeting chronic low-grade inflammation in middle-age and older adults:a systematic review and meta-analysis[J].Ageing Research Reviews,2018,46:42-59.
[23] CHEN S W,ZHU J,ZUO S,et al.Protective effect of hydrogen sulfide on TNF-α and IFN-γ-induced injury of Intestinal epithelial barrier function in Caco-2 monolayers[J].Inflammation Research,2015,64(10):789-797.

[24] 杨凤娟,曾祥芳,谯仕彦.罗伊氏乳杆菌I5007对新生仔猪肠道形态、二糖酶活性和紧密连接蛋白表达的影响[J].中国农业科学,2014,47(22):4506-4515.
[25] 李雪莉,虞徳夫,王超,等.植物乳杆菌制剂对断奶仔猪肠道黏膜功能和微生物菌群及短链脂肪酸的影响[J].南京农业大学学报,2018,41(3):504-510.

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