动物营养学报 >

2019 , Vol. 31 >Issue 9: 4251 - 4261

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.039

饲料科学与技术 Feed science and technology

血根碱对脂多糖免疫应激断奶仔猪生长性能、免疫功能及肠道健康的影响

  • 康保聚 ,
  • 陈家顺 ,
  • 金顺顺 ,
  • 田军权 ,
  • 王鹏 ,
  • 符晨星 ,
  • 伍小松 ,
  • 姚康
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  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 长沙 410025;
    3. 中国科学院大学, 北京 100049
康保聚(1991-),男,河南商丘人,硕士研究生,动物营养与饲料科学专业。E-mail:1456427701@qq.com

收稿日期: 2019-03-12

  网络出版日期: 2019-09-17

基金资助

国家自然科学基金青年科学基金项目（31702126）；湖南省教育厅科学研究项目优秀青年项目（17B121）；中科院"百人计划"项目

收起

Effects of Sanguinarine on Growth Performance, Immune Function and Gut Heath of Immune-Stressed Weaned Piglets Challenged with Lipopolysaccharide

  • KANG Baoju ,
  • CHEN Jiashun ,
  • JIN Shunshun ,
  • TIAN Junquan ,
  • WANG Peng ,
  • FU Chenxing ,
  • WU Xiaosong ,
  • YAO Kang
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410025, China;
    3. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2019-03-12

  Online published: 2019-09-17

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摘要

本试验旨在研究血根碱（SAG）对脂多糖（LPS）免疫应激断奶仔猪生长性能、免疫功能及肠道健康的影响。试验选取（21±1）日龄、平均体重为（6.20±0.09）kg的健康"杜×长×大"断奶仔猪24头，按体重相近原则随机分为4组，分别为对照（CON）组（基础饲粮）、LPS组（基础饲粮+腹腔注射100 μg/kg BW LPS）、SAG组（基础饲粮+50 mg/kg血根碱）和SAG+LPS组（基础饲粮+50 mg/kg血根碱+腹腔注射100 μg/kg BW LPS），每组6个重复，每个重复1头猪。试验采用2×2双因子设计，主因子为血根碱（饲粮中添加、不添加）和LPS（腹腔注射、不注射）。分别于试验第14、21天给断奶仔猪腹腔注射LPS或等量的生理盐水。试验期21 d。结果表明：1）LPS攻毒前（1~14 d），饲粮中添加血根碱显著提高断奶仔猪的平均日采食量（ADFI）和平均日增重（ADG）（P<0.05），显著降低料重比（F/G）（P<0.05）。攻毒期间（15~21 d），LPS攻毒显著降低ADG和ADFI（P<0.05），显著提高F/G（P<0.05）；饲粮中添加血根碱显著提高ADFI和ADG（P<0.05），显著降低F/G（P<0.05）。2）攻毒结束后，饲粮中添加血根碱和LPS攻毒均显著提高断奶仔猪的血清免疫球蛋白G（IgG）和免疫球蛋白M（IgM）含量（P<0.05）。与LPS组相比，SAG+LPS组的血清IgG和IgM含量显著增加（P<0.05）。3）攻毒结束后，LPS攻毒显著提高断奶仔猪的血清白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α（TNF-α）含量（P<0.05），显著降低血清IL-10含量（P<0.05）。饲粮中添加血根碱显著降低血清IL-1β、IL-6和TNF-α含量（P<0.05），显著增加血清IL-10含量（P<0.05）。与LPS组比，SAG+LPS组的血清IL-1β、IL-6和TNF-α含量显著降低（P<0.05），血清IL-10含量显著增加（P<0.05）。饲粮中添加血根碱与LPS攻毒的交互作用对血清TNF-α含量有显著影响（P<0.05）。4）攻毒结束后，LPS攻毒显著提高断奶仔猪的血清D-乳酸（D-LA）含量和二胺氧化酶（DAO）活性（P<0.05）。饲粮中添加SAG显著降低血清D-LA含量和DAO活性（P<0.05）。与LPS组比，SAG+LPS组血清D-LA含量和DAO活性显著降低（P<0.05）。饲粮中添加血根碱与LPS攻毒的交互作用对血清D-LA含量和DAO活性有显著影响（P<0.05）。5）攻毒结束后，LPS攻毒显著降低断奶仔猪的十二指肠、回肠绒毛高度（VH）及绒毛高度/隐窝深度（V/C），显著提高十二指肠隐窝深度（CD）（P<0.05）。饲粮中添加血根碱显著提高十二指肠、空肠、回肠VH及V/C，显著降低十二指肠CD（P<0.05）。与LPS组相比，SAG+LPS组的十二指肠、空肠和回肠VH和V/C显著增加（P<0.05），十二指肠CD显著降低（P<0.05）。由此可见，饲粮中添加血根碱可以缓解断奶仔猪免疫应激导致的生长性能下降，提高血清IgG和IgM含量，抑制血清IL-1β、IL-6、TNF-α含量的增加和IL-10含量的降低，进而增强其免疫抵抗能力，缓解肠道通透性增加，并在一定程度上改善小肠黏膜形态结构，从而使断奶仔猪的肠道健康得到改善。

关键词： 血根碱; 断奶仔猪; 脂多糖; 生长性能; 免疫功能; 肠道健康

本文引用格式

康保聚 , 陈家顺 , 金顺顺 , 田军权 , 王鹏 , 符晨星 , 伍小松 , 姚康 . 血根碱对脂多糖免疫应激断奶仔猪生长性能、免疫功能及肠道健康的影响[J]. 动物营养学报, 2019 , 31(9) : 4251 -4261 . DOI: 10.3969/j.issn.1006-267x.2019.09.039

Abstract

This experiment was conducted to investigate the effects of sanguinarine (SAG) on growth performance, immune function and gut heath of immune-stressed weaned piglets challenged with lipopolysaccharide (LPS). Twenty-four healthy "Duroc×Landrace×Yorkshire" weaned piglets at (21±1) days of age with average body weight of (6.20±0.09) kg were randomly assigned to 4 groups with 6 replicates per group and 1 piglet per replicate according to similar body weight. The 4 groups were control (CON) group (basal diet), LPS group (basal diet and injected intraperitoneally with 100 μg/kg BW LPS), SAG group (basal diet supplemented with 50 mg/kg SAG) and SAG+LPS group (basal diet supplemented with 50 mg/kg SAG and injected intraperitoneally with 100 μg/kg BW LPS), respectively. A 2×2 double-factor design was used, and the main factors were SAG (added or not in diet) and LPS (injected intraperitoneally or not). Weaned piglets were injected intraperitoneally with 100 μg/kg BW LPS or an equivalent amount of sterile saline at days 14 and 21. The trail lasted for 21 days. The results showed as follows:1) before challenged with LPS (days 1 to 14), dietary SAG significantly increased average daily feed intake (ADFI) and average daily gain (ADG) of weaned piglets and significantly decreased the ratio of feed to gain (F/G) (P<0.05). During the period of challenged with LPS (days 15 to 21), LPS challenge significantly decreased ADG and ADFI and significantly increased F/G (P<0.05), and dietary SAG significantly increased ADFI and ADG and significantly decreased F/G (P<0.05). 2) After the LPS challenge, both dietary SAG and LPS challenge significantly increased the contents of immunoglobulin G (IgG) and immunoglobulin M (IgM) in serum of weaned piglets (P<0.05). Compared with LPS group, the contents of IgG and IgM in serum were significantly increased in SAG+LPS group (P<0.05). 3) After the LPS challenge, LPS challenge significantly increased the contents of interleukin (IL)-1β、IL-6 and tumor necrosis factor-α (TNF-α) in serum of weaned piglets (P<0.05), and significantly decreased the content of IL-10 in serum (P<0.05). Dietary SAG significantly decreased the contents of IL-1β、IL-6 and TNF-α in serum (P<0.05), and significantly increased the content of IL-10 in serum (P<0.05). Compared with LPS group, the contents of IL-1β、IL-6 and TNF-α in serum were significantly decreased and the content of IL-10 in serum was significantly increased in the SAG+LPS group (P<0.05). The interaction of dietary SAG and LPS challenge had a significant effect on the content of TNF-α in serum (P<0.05). 4) After the LPS challenge, LPS challenge significantly increased the content of D-lactate (D-LA) and the activity of diamine oxidase (DAO) in serum of weaned piglets (P<0.05). Dietary SAG significantly decreased the content of D-LA and the activity of DAO in serum (P<0.05). Compared with LPS group, the content of D-LA and the activity of DAO in serum were significantly decreased in SAG+LPS group (P<0.05). The interaction of dietary SAG and LPS challenge had a significant effects on the content of D-LA and the activity of DAO in serum (P<0.05). 5) After the LPS challenge, LPS challenge significantly decreased villus height (VH) and the ratio of villus height to crypt depth (V/C) of duodenum and ileum of weaned piglets (P<0.05), and significantly increased crypt depth (CD) of duodenum (P<0.05). Dietary SAG significantly increased VH and V/C of duodenum, jejunum and ileum (P<0.05), and significantly decreased CD of duodenum (P<0.05). Compared with LPS group, VH and V/C were significantly increased of duodenum, jejunum and ileum (P<0.05), and CD of duodenum was significantly decreased (P<0.05). In conclusion, dietary SAG can alleviate the decline of growth performance of weaned piglets caused by immunological stress, increase the contents of IgG and IgM in serum, inhabit the increase of IL-1β, IL-6, TNF-α contents and the decrease of IL-10 content in serum, and then improve immune resistance, alleviate the increase of intestinal permeability and improve intestinal mucosal morphology, and improve the intestinal health of weaned piglets.

Key words： sanguinarine; weaned piglets; lipopolysaccharide; growth performance; immune function; gut health

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