动物营养学报 >

2019 , Vol. 31 >Issue 9: 4286 - 4295

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.043

饲料科学与技术 Feed science and technology

竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数及血清免疫和抗氧化相关指标的影响

  • 侯昆 ,
  • 童津津 ,
  • 楚康康 ,
  • 熊本海 ,
  • 蒋林树
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  • 1. 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206;
    2. 北京奶牛中心, 首农集团, 北京 100192;
    3. 中国农业科学院北京畜牧兽医研究所, 北京 100193
侯昆(1996-),男,北京人,硕士研究生,研究方向为反刍动物营养与免疫。E-mail:1031520946@qq.com

收稿日期: 2019-04-16

  网络出版日期: 2019-09-17

基金资助

国家自然科学基金项目（31772629，31702302，31802091）；2019年北京农学院学位与研究生教育改革与发展项目（2019YJS031）

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Effects of Bamboo Leaf Flavonoids and Artemisia annua Extract on Milk Performance, Milk Somatic Cell Count and Serum Immune and Antioxidant Related Indexes of Dairy Cows with Subclinical Mastitis

  • HOU Kun ,
  • TONG Jinjin ,
  • CHU Kangkang ,
  • XIONG Benhai ,
  • JIANG Linshu
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  • 1. Beijing Institute of Animal Science and Technology, Beijing Key Laboratory of Cow Nutrition, Beijing 102206, China;
    2. Beijing Dairy Cattle Center, Beijing Capital Agribusiness Group, Beijing 100192, China;
    3. Beijing Institute of Animal Husbandry and Veterinary Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2019-04-16

  Online published: 2019-09-17

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摘要

本试验旨在研究竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数（SCC）及血清免疫和抗氧化相关指标的影响。将2~4胎次、体重（590±15）kg、产奶量和泌乳天数相近的18头患隐性乳房炎荷斯坦奶牛[SCC为（41.31±0.49）×104个/mL]随机分为3组，每组6头，进行饲养试验。其中，对照组奶牛饲喂全混合日粮（TMR），竹叶黄酮组与青蒿提取物组奶牛除饲喂TMR外，再分别投喂30 g/d的竹叶黄酮和60 g/d的青蒿提取物。饲养试验进行35 d，其中预试期7 d，饲喂期14 d，停止饲喂期14 d。分别在试验第1、14、28天晨饲前采集血样、奶样，并记录产奶量，分别进行乳中SCC、乳成分以及血清免疫相关因子含量和抗氧化指标的检测。结果显示：1）与对照组相比，竹叶黄酮组和青蒿提取物组奶牛的产奶量分别增加了8.95%和13.24%（P>0.05），4%乳脂校正乳产量和第14天乳蛋白率显著提高（P<0.05），第14天乳中SCC显著降低（P<0.05）。2）竹叶黄酮组与青蒿素提取物组奶牛第14天血清中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）含量均显著低于对照组（P<0.05）；此外，青蒿提取物组奶牛第14天血清中白细胞介素-2（IL-2）含量显著高于对照组（P<0.05）。3）与对照组相比，竹叶黄酮组与青蒿提取物组奶牛第14天血清中丙二醛（MDA）含量显著下降（P<0.05），第14天血清中超氧化物歧化酶（SOD）活性有上升趋势（P=0.06）。综上可知，竹叶黄酮与青蒿提取物可在一定程度上提高奶牛的产奶性能，增强机体抗氧化能力与免疫功能，并可降低患隐性乳房炎奶牛乳中SCC。

关键词： 竹叶黄酮; 青蒿提取物; 奶牛; 隐性乳房炎; 抗氧化能力; 免疫功能

本文引用格式

侯昆 , 童津津 , 楚康康 , 熊本海 , 蒋林树 . 竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数及血清免疫和抗氧化相关指标的影响[J]. 动物营养学报, 2019 , 31(9) : 4286 -4295 . DOI: 10.3969/j.issn.1006-267x.2019.09.043

Abstract

This experiment was conducted to investigate the effects of bamboo leaf extract and Artemisia annua extract on milk performance, milk somatic cell count (SCC)and serum immune and antioxidant related indexes of dairy cows with subclinical mastitis. Eighteen Chinese Holstein cows with subclinical mastitis[SCC was (41.31+0.49)×104 cell/mL] with similar days in milk, similar milk yield, body weight of (590±15) kg, 2 to 4 parities were selected and randomly divided into 3 groups with 6 cows per group for feeding experiment. Cows in control group were a total mixed ration (TMR), while cows in bamboo leaf flavones group and Artemisia annua extract group were also fed TMR, and supplementary fed with bamboo leaf flavones (30 g/d) and Artemisia annua extract (60 g/d), respectively. The feeding experiment was conducted for 35 days, including pre-test period of 7 days, feeding period of 14 days and post feeding period of 14 days. Blood and milk samples were collected on day 1, 14, 28 to measure milk SCC, milk composition and serum immune-related factor contents and antioxidant indexes, and the milk yield was recorded. The results showed as follows:1) compared with the control group, the milk yield in bamboo leaf flavones group and Artemisia annua extract group was increased by 8.95% and 13.24% (P>0.05), respectively, the 4% fat corrected milk (FCM) yield and milk protein percentage on day 14 were significantly increased (P<0.05), and the milk SCC on day 14 was significantly decreased (P<0.05). 2) The contents of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum on day 14 in bamboo leaf flavones group and Artemisia annua extract group were significantly lower than those in control group (P<0.05); otherwise, the serum interleukin-2 (IL-2) content in serum in Artemisia annua extract group was significantly higher than that in control group (P<0.05). 3) Compared with the control group, the serum malondialdehyde (MDA) content on day 14 was decreased significantly (P<0.05), and serum superoxide dismutase (SOD) activity on day 14 tended to be increased both in bamboo leaf flavones group and Artemisia annua extract group (P=0.06). In conclusion, both bamboo leaf flavonoids and Artemisia annua extract can improve the mill performance, enhance the immune function and antioxidant capacity of dairy cows, and decrease the milk SCC of dairy cows with subclinical mastitis.

Key words： bamboo leaf flavonoids; Artemisia annua extract; antioxidant capacity; immune function; dairy cows; subclinical mastitis

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