无柄灵芝的鉴定及其在SD大鼠饲粮中应用价值的评估

付饶; 张辉金; 夏旻灏; 梁姝婕; 臧旭鹏; 余小华; 沈水宝; 董尚智; 冯定远; 左建军

doi:10.3969/j.issn.1006-267x.2019.09.046

动物营养学报 >

2019 , Vol. 31 >Issue 9: 4314 - 4321

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.09.046

饲料科学与技术 Feed science and technology

无柄灵芝的鉴定及其在SD大鼠饲粮中应用价值的评估

付饶 ,
张辉金 ,
夏旻灏 ,
梁姝婕 ,
臧旭鹏 ,
余小华 ,
沈水宝 ,
董尚智 ,
冯定远 ,
左建军

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1. 华南农业大学动物科学学院, 广州 510642;
2. 广西大学动物科学技术学院, 南宁 530004;
3. 珠海市现代农业发展中心, 珠海 519000

付饶(1994-),女,湖北十堰人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:1204950413@qq.com

收稿日期: 2019-03-01

网络出版日期: 2019-09-17

基金资助

国家自然科学基金项目（31872390）；广东省现代饲料产业创新团队饲料成份与营养价值岗位专家项目（2018LM1120）；广东省科技计划项目（2016A020210104）；珠海市高等教育建设项目

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Identification of Sessile Ganoderma Lucidum and Evaluation of Its Application Value in SD Rats Diet

FU Rao ,
ZHANG Huijin ,
XIA Minhao ,
LIANG Shujie ,
ZANG Xupeng ,
YU Xiaohua ,
SHEN Shuibao ,
DONG Shangzhi ,
FENG Dingyuan ,
ZUO Jianjun

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1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
3. Zhuhai Modern Agriculture Development Center, Zhuhai 519000, China

Received date: 2019-03-01

Online published: 2019-09-17

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摘要

本试验旨在研究液体培养的无柄灵芝菌液和菌丝对SD大鼠生长性能和营养物质沉积的影响，评估其在饲粮中的应用价值。通过对灵芝菌DNA的ITS序列的分析，对灵芝菌属进行鉴定。选取36只健康的3周龄雄性SD大鼠，随机分成3组：灌喂马铃薯葡萄糖水组（对照组）、灌喂无柄灵芝菌液组（菌液组）和灌喂无柄灵芝菌丝组（菌丝组），每组4个重复，每个重复3只大鼠，按照10 mL/（kg BW·d）连续灌喂4周。结果表明：与对照组相比，菌液组平均日增重显著提高（P<0.10），菌丝组平均日采食量显著降低（P<0.10），菌液组和菌丝组饲料转化率均无显著差异（P>0.10）；菌液组和菌丝组器官指数和背最长肌中肌苷酸含量均无显著差异（P>0.05），但菌液组背最长肌中总蛋白以及天冬氨酸、丝氨酸、谷氨酸、甘氨酸、丙氨酸、缬氨酸、蛋氨酸、异亮氨酸、酪氨酸、苯丙氨酸、组氨酸、精氨酸等12种氨基酸的含量显著提高（P<0.05）。综上，灌喂无柄灵芝菌液、菌丝对SD大鼠的生长性能有一定影响，但灌喂无柄灵芝菌液对肌肉中总蛋白、风味氨基酸的沉积具有促进作用。

关键词： 无柄灵芝; SD大鼠; 生长性能; 器官指数; 营养物质沉积

本文引用格式

付饶 , 张辉金 , 夏旻灏 , 梁姝婕 , 臧旭鹏 , 余小华 , 沈水宝 , 董尚智 , 冯定远 , 左建军 . 无柄灵芝的鉴定及其在SD大鼠饲粮中应用价值的评估[J]. 动物营养学报, 2019 , 31(9) : 4314 -4321 . DOI: 10.3969/j.issn.1006-267x.2019.09.046

Abstract

This experiment was aimed to investigate the effects of liquid cultured sessile Ganoderma lucidum solution and mycelium on growth performance and nutrient deposition in SD rats, and evaluate its application value in diets. The Ganoderma genus was identified by analysis of the ITS sequence of Ganoderma lucidum DNA. Thirty-six healthy 3-week-old male SD rats were randomly divided into three groups:feeding potato glucose water group (control group), feeding sessile Ganoderma lucidum solution group (solution group) and feeding sessile Ganoderma lucidum mycelium group (mycelium group), with 4 replicates per group and 3 rats per replicate, and fed 4 weeks at a dose of 10 mL/(kg BW·d). The results showed as follows:compared with the control group, the average daily weight gain in solution group increased significantly (P<0.10), the average daily feed intake in mycelium group significantly decreased (P<0.10), while there was no significant effect on the feed conversion rate in solution group and mycelium group (P>0.10); the organ indexes and inosine acid content of Longissimus dorsi in solution group and mycelium group had no significant difference (P>0.05), but the contents of total protein and aspartic acid, serine, glutamate, glycine, alanine, valine, methionine, isoleucine, tyrosine, phenylalanine, histidine and arginine of Longissimus dorsi in solution group significantly increased (P<0.05). In conclusion, feeding sessile Ganoderma lucidum solution and mycelium have no significant effect on the growth of SD rats, but Ganoderma lucidum solution can promote the deposition of total protein and flavor amino acids in muscle.

Key words： sessile Ganoderma lucidum; SD rats; growth performance; organ index; nutrient deposition

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