反刍与草食动物营养 Ruminant and herbivore nutrition

醋酸棉酚对绵羊瘤胃液中原虫数量和种群多样性的影响

  • 王彩蝶 ,
  • 李钰琪 ,
  • 屯妮萨·麦提赛伊迪 ,
  • 杨红建 ,
  • 杨开伦
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  • 1. 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052;
    2. 中国农业大学动物科技学院, 动物营养学国家重点实验室, 北京 100193
王彩蝶(1989-),女,新疆精河人,博士研究生,从事草食动物营养与代谢研究。E-mail:1491673671@qq.com

收稿日期: 2019-04-09

  网络出版日期: 2019-10-17

基金资助

中国农业大学-新疆农业大学联合基金(2017TC002);2017年度新疆农业大学研究生科研创新项目(XJAUGRI2017006)

Effects of Gossypol Acetic Acid on Number and Population Diversity of Protozoa in Rumen Fluid of Sheep

  • WANG Caidie ,
  • LI Yuqi ,
  • TUNNISA·Maitisaiyidi ,
  • YANG Hongjian ,
  • YANG Kailun
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  • 1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China

Received date: 2019-04-09

  Online published: 2019-10-17

摘要

本试验旨在研究醋酸棉酚对绵羊瘤胃液中原虫数量和种群多样性的影响。选取8只安装有永久性瘤胃瘘管的哈萨克羊母羊,随机分为2组,分别为试验组和对照组,每组4只。试验期内(第1~47天)对照组绵羊饲喂基础饲粮;试验期第1~26天,试验组绵羊在每千克基础饲粮中添加醋酸棉酚361 mg,第27~47天醋酸棉酚添加量增加至722 mg。分别于试验第20、37和47天采集绵羊瘤胃液样品,应用显微镜计数方法结合高通量测序技术,检测瘤胃液样品中原虫数量和种群多样性。结果显示:1)醋酸棉酚显著降低了第20天瘤胃液中原虫数量(P<0.05),但对第37和47天瘤胃液中原虫数量无显著影响(P>0.05);2)绵羊瘤胃液原虫样品稀释曲线显示,测序已经覆盖了瘤胃液中绝大部分的原虫;3)饲喂醋酸棉酚不同天数对瘤胃液中原虫种群alpha多样性无显著影响(P>0.05);4)主成分分析(PCA)显示,醋酸棉酚对瘤胃液原虫区系结构组成无明显影响;5)第37天,在属水平上,试验组绵羊瘤胃液头毛属相对丰度显著高于对照组(P<0.05),但瘤胃液中其他原虫属相对丰度与对照组相比无显著变化(P>0.05)。综上所述,在本试验条件下,每千克基础饲粮中添加醋酸棉酚361 mg,在第20天时显著降低了绵羊瘤胃液中原虫数量;每千克基础饲粮中醋酸棉酚添加量增加至722 mg,对绵羊瘤胃液原虫数量没有显著影响;醋酸棉酚对绵羊瘤胃液中原虫种群多样性无显著影响。

本文引用格式

王彩蝶 , 李钰琪 , 屯妮萨·麦提赛伊迪 , 杨红建 , 杨开伦 . 醋酸棉酚对绵羊瘤胃液中原虫数量和种群多样性的影响[J]. 动物营养学报, 2019 , 31(10) : 4582 -4589 . DOI: 10.3969/j.issn.1006-267x.2019.10.022

Abstract

The aim of this experiment was to evaluate the effects of gossypol acetic acid (GAA) on the number and population diversity of protozoa in rumen fluid of sheep. Eight Kazakh female sheep with permanently rumen fistula were randomly divided into two groups (control group and trial group) with four sheep in each group. The sheep in the control group were fed a basal diet during trial period (from 1st to 47th day), while the sheep in the trial group were fed the basal diet supplemented with 361 mg/kg GAA from 1st to 26th day, and 722 mg/kg GAA from 27th to 47th day of the trial period. Rumen fluid samples at days 20, 37 and 47 were collected to detected the number and population diversity of protozoa by microscope count method combined with high throughput sequencing technique. The results showed as follows:GAA significantly reduced the number of protozoa in rumen fluid at day 20 (P<0.05), but there was no significant effect at other time points (days 37 and 47) (P>0.05). 2) The rarefaction curve of rumen fluid protozoa samples showed that the sequencing had covered most protozoa in rumen fluid. 3) GAA had no significant effect on the alpha diversity of protozoa population in rumen fluid at different time days (P>0.05). 4)The results of principal component analysis (PCA) showed that GAA had no obvious effect on the structure of protozoa population in rumen fluid. 5) At genus level, compared with the control group, the relative abundance of Ophryoscolex at day 37 in rumen fluid of the trial group was significantly increased (P<0.05), while the relative abundances of other protozoa genus in rumen fluid were not significantly changed (P>0.05). In conclusion, under the conditions of this experiment, basal diet supplemented with 361 mg/kg GAA significantly reduces the number of protozoa in rumen fluid of sheep at day 20. There is no significant effect on the number of protozoa in rumen fluid of sheep when increasing GAA to 722 mg/kg. GAA has no significant effect on diversity of protozoa population in rumen fluid of sheep.

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