水产营养 Aquaculture Nutrition

N-氨甲酰谷氨酸对花鲈生长性能、形体指标和血浆生化指标的影响

  • 黄皓琰 ,
  • 郁欢欢 ,
  • 梁晓芳 ,
  • 郑银桦 ,
  • 王春平 ,
  • 吴秀峰 ,
  • 陈沛 ,
  • 薛敏
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  • 1. 中国农业科学院饲料研究所, 国家水产饲料安全评价基地, 北京 100081;
    2. 亚太兴牧(北京)科技有限公司, 北京 100193;
    3. 农业部饲料生物技术重点开放实验室, 北京 100081
黄皓琰(1993-),女,山东日照人,硕士研究生,研究方向为水产动物营养与饲料科学。E-mail:1119288183@qq.com

收稿日期: 2019-03-22

  网络出版日期: 2019-10-17

基金资助

国家重点研发计划项目(2018YFD0900400,2016YFF0201800);国家自然科学基金项目(31372539,31572631);国家重点基础研究发展计划项目(2014CB138600);北京市现代农业产业技术体系(SCGWZJ 20171103-1);中国农业科学院基本科研业务费(1610382016010);中国农业科学院创新工程项目(CAAS-ASTIP-2017-FRI-08)

Effects of N-Carbamylglutamate on Growth Performance, Morphometric Parameters and Plasma Biochemical Parameters of Japanese Seabass (Lateolabrax japonocus)

  • HUANG Haoyan ,
  • YU Huanhuan ,
  • LIANG Xiaofang ,
  • ZHENG Yinhua ,
  • WANG Chunping ,
  • WU Xiufeng ,
  • CHEN Pei ,
  • XUE Min
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  • 1. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Beijing Animore Science & Technology Co., Ltd., Beijing 100193, China;
    3. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2019-03-22

  Online published: 2019-10-17

摘要

本试验通过在饲料中添加不同水平的N-氨甲酰谷氨酸(NCG),研究其对花鲈生长性能、形体指标和血浆生化指标的影响,以确定NCG在花鲈饲料中的适宜添加水平。在基础饲料中分别添加0(对照)、120、240、360、480、720 mg/kg的NCG,制成6种试验饲料,分别命名为N0、N120、N240、N360、N480和N720,饲喂平均初始体重为(11.67±0.02)g的花鲈10周。每种试验饲料投喂6个养殖桶(重复),每个养殖桶放养30尾花鲈。结果表明:各组之间终末均重、增重率、脂肪沉积率、特定生长率、脏体比和肝体比无显著差异(P>0.05)。N240组花鲈具有最高的蛋白质沉积率和最低的腹脂率,其蛋白质沉积率显著高于除N360组外的其他各组(P<0.05),腹脂率显著低于N480组(P<0.05)。肝脏脂肪含量在N360组最低,显著低于N0组和N120组(P<0.05)。血浆葡萄糖、总胆固醇、低密度脂蛋白胆固醇含量以及谷丙转氨酶、谷草转氨酶活性各组间无显著差异(P>0.05)。N120组血浆尿素氮含量显著低于N0组、N480组和N720组(P<0.05)。N120组血浆甘油三酯含量显著低于N0组和N360组(P<0.05)。N240组血浆高密度脂蛋白胆固醇含量显著高于N360组和N720组(P<0.05)。血浆一氧化氮含量以N360组最高,显著高于N480组(P<0.05)。由上述结果可知,在花鲈饲料中添加适量NCG能够促进鱼体蛋白质沉积,降低腹腔和肝脏脂肪蓄积,改善蛋白质和脂肪代谢。分别以蛋白质沉积率、腹脂率为指标,通过折线模型分析得出,花鲈蛋白质沉积率最高时NCG添加水平为219.2 mg/kg,腹脂率最低时NCG添加水平为214.3 mg/kg。综合考虑得出,花鲈饲料中的NCG适宜添加水平为214.3~219.2 mg/kg。

本文引用格式

黄皓琰 , 郁欢欢 , 梁晓芳 , 郑银桦 , 王春平 , 吴秀峰 , 陈沛 , 薛敏 . N-氨甲酰谷氨酸对花鲈生长性能、形体指标和血浆生化指标的影响[J]. 动物营养学报, 2019 , 31(10) : 4590 -4601 . DOI: 10.3969/j.issn.1006-267x.2019.10.023

Abstract

A 10-week growth trial was to determine the effects of different supplemental levels of N-carbamylglutamate (NCG) in the diet on growth performance, morphometric parameters and plasma biochemical parameters of Japanese seabass (Lateolabrax japonocus), and to determine the optimum requirement of NCG for Japanese seabass. Six experimental diets were designed with NCG supplement levels of 0 (N0), 120 (N120), 240 (N240), 360 (N360), 480 (N480) and 720 mg/kg (N720), respectively. Each diet was fed to 6 replicates with 30 Japanese seabass[initial body weight=(11.67±0.02) g] in each replicate. The results showed that no significant differences in final body weight (FBW), weight gain rate (WGR), fat retention ratio (FRR), special growth rate (SGR), viscerasomatic index (VSI) and hepatosomatic index (HSI) were observed among groups (P>0.05). The N240 group had the highest protein retention ratio (PRR) and the lowest abdominal fat percentage (AFP), and the PRR was significantly higher than that in the groups except N360 group (P<0.05), while the AFP was significantly lower than that in the N480 group (P<0.05). Hepatic fat content was the lowest in the N360 group, which was significantly lower than that in the N0 and N120 groups (P<0.05). There were no significant differences in plasma glucose (GLU), total cholesterol (TC), low density lipoprotein cholesterin (LDL-C) contents and aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities (P>0.05). Plasma urea nitrogen (UN) content in the N120 group was significantly lower than that in the N0, N480 and N720 groups (P<0.05). Plasma triglyceride (TG) content in the N120 group was significantly lower than that in the N0 and N360 groups (P<0.05). Plasma high density lipoprotein cholesterin (HDL-C) content in the N240 group was significantly higher than that in the N360 and N720 groups (P<0.05). Plasma nitric oxide (NO) content was the highest in the N360 group which was significantly higher than that in the N480 group (P<0.05). The results indicate that appropriate dietary NCG supplementation can promote the protein retention, decrease the fat accumulation in liver and abdomen, and improve the metabolism of protein and lipid in Japanese seabass. According to the broken-line models, taking PRR and AFP as evaluation indexes, the highest PRR can be obtained when dietary NCG supplemental level is 219.2 mg/kg, and the lowest AFP can be obtained when dietary NCG supplemental level is 214.3 mg/kg. By comprehensive analysis, optimal dietary NCG supplemental level in the diet of Japanese seabass is 214.3 to 219.2 mg/kg.

参考文献

[1] YU H H,HAN F,XUE M,et al.Efficacy and tolerance of yeast cell wall as an immunostimulant in the diet of Japanese seabass (Lateolabrax japonicus)[J].Aquaculture,2014,432:217-224.
[2] 林利民,胡家财,洪惠馨.鲈鱼(Lateolabrax japonicus)人工配合饲料中蛋白质最适含量的研究[J].厦门水产学院学报,1994,16(1):6-10.
[3] 高淳仁,刘庆慧,梁亚全,等.鲈鱼幼鱼人工配合饲料中蛋白质、脂肪适宜含量的研究[J].海洋水产研究,1998,19(1):81-85.
[4] 周凡,邵庆均.鱼类必需氨基酸营养研究进展[J].饲料与畜牧,2010(8):20-26.
[5] NRC.Nutrient requirements of fish and shrimp[S].Washington,D.C.:National Academies Press, 2011.
[6] NGAMSNAE,DE SILVA S S,GUNASEKERA R.Arginine and phenylalanine requirement of juvenile silver perch Bidyanus bidyanus and validation of the use of body amino acid composition for estimating individual amino acid requirements[J].Aquaculture Nutrition,2015,5(3):173-180.
[7] KAUSHIK S J,SEILIEZ I.Protein and amino acid nutrition and metabolism in fish:current knowledge and future needs[J].Aquaculture Research,2010,41(3):322-332.  
[8] CHENG Z Y,GATLIN Ⅲ D M,BUENTELLO A.Dietary supplementation of arginine and/or glutamine influences growth performance,immune responses and intestinal morphology of hybrid striped bass (Morone chrysops×Morone saxatilis)[J].Aquaculture,2012,362/363:39-43.
[9] ZHOU Q C,JIN M,ELMADA Z C,et al.Growth,immune response and resistance to Aeromonas hydrophila of juvenile yellow catfish,Pelteobagrus fulvidraco,fed diets with different arginine levels[J].Aquaculture,2015,437:84-91.
[10] JONES J D.Lysine-arginine antagonism in the chick[J].The Journal of Nutrition,1964,84(4):313-321.  
[11] 刘雅倩.N-氨甲酰谷氨酸合成及其生理功能研究[D].硕士学位论文.长沙:长沙理工大学,2011.
[12] 曹洪战,陈楠,芦春莲.N-氨甲酰谷氨酸对哺乳仔猪生长及内源精氨酸合成代谢的影响[J].畜牧兽医学报,2013,44(11):1844-1850.
[13] WU G Y,KNABE D A,KIM S W.Arginine nutrition in neonatal pigs[J].The Journal of Nutrition,2004,134(Suppl.10):2783S-2790S.
[14] WU X,ZHANG Y,LIU Z,et al.Effects of oral supplementation with glutamate or combination of glutamate and N-carbamylglutamate on intestinal mucosa morphology and epithelium cell proliferation in weanling piglets[J].Journal of Animal Science,2012,90 Suppl 4:337-339.
[15] WU X,RUAN Z,GAO Y L,et al.Dietary supplementation with L-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn- and soybean meal-based diet[J].Amino Acids,2010,39(3):831-839.  
[16] ZHANG H,ZHAO F F,PENG A,et al.Effects of dietary L-arginine and N-carbamylglutamate supplementation on intestinal integrity,immune function,and oxidative status in intrauterine-growth-retarded suckling lambs[J].Journal of Agricultural and Food Chemistry,2018,66(16):4145-4154.  
[17] 尚晓迪,陈春秀,贾磊,等.N-氨甲酰谷氨酸对大菱鲆幼鱼生长性能的影响[J].饲料研究,2017(3):35-38.
[18] 程炜轩,张丽,许国焕,等.N-氨甲酰谷氨酸对罗非鱼幼鱼生长、血液氨基酸组成及脂肪沉积的影响[J].水生生物学报,2015,39(3):490-497.
[19] WANG L S,LI J N,WANG C A,et al.Effect of N-carbamoylglutamate supplementation on the growth performance,antioxidant status and immune response of mirror carp (Cyprinus carpio) fed an arginine-deficient diet[J].Fish & Shellfish Immunology,2019,84:280-289.
[20] JOBLING M.National Research Council (NRC):nutrient requirements of fish and shrimp[J].Aquaculture International,2012,20(3):601-602.  
[21] BAKER D H.Lysine,arginine,and related amino acids:an introduction to the 6th amino acid assessment workshop[J].The Journal of Nutrition,2007,137(6 Suppl.2):1599S-1601S.
[22] CVANCARA V A.Studies on tissue arginase and ureogenesis in fresh-water teleosts[J].Comparative Biochemistry and Physiology,1969,30(3):489-496.  
[23] LIANG X F,HAN J,XUE M,et al.Growth and feed intake regulation responses to anorexia,adaptation and fasting in Japanese seabss,Lateolabrax japonicas when fishmeal is totally replaced by plant protein[J].Aquaculture,2019,498:528-538.
[24] 苏传福.花鲈营养需求的研究进展[J].饲料研究,2005,14(4):35-38.
[25] 王远红,吕志华,高天翔,等.不同海域中国花鲈营养成分的比较研究[J].青岛海洋大学学报,2003,33(4):531-536.
[26] 王远红,吕志华,高天翔,等.中国花鲈与日本花鲈营养成分的研究[J].海洋水产研究,2003,24(2):35-39.
[27] TIBALDI E,TULLI F,LANARI D.Arginine requirement and effect of different dietary arginine and lysine levels for fingerling sea bass (Dicentrarchus labrax)[J].Aquaculture,1994,127(2/3):207-218.
[28] LUZZANA U,HARDY R W,HALVER J E.Dietary arginine requirement of fingerling coho salmon (Oncorhynchus kisutch)[J].Aquaculture,1998,163(1/2):137-150.
[29] AHMED I,KHAN M A.Dietary arginine requirement of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)[J].Aquaculture Nutrition,2004,10(4):217-225.  
[30] ALAM M S,TESHIMA S I,KOSHIO S,et al.Arginine requirement of juvenile Japanese flounder Paralichthys olivaceus estimated by growth and biochemical parameters[J].Aquaculture,2002,205(1/2):127-140.
[31] FRANK J W,ESCOBAR J,NGUYEN H V,et al.Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets[J].The Journal of Nutrition,2007,137(2):315-319.  
[32] 尚晓迪,陈春秀,贾磊,等.N-氨甲酰谷氨酸对大菱鲆幼鱼营养组成及免疫功能的影响[J].饲料工业,2017,38(18):5-9.
[33] FAN Z,SHAO Q J,XIAO J X,et al.Effects of dietary arginine and lysine levels on growth performance,nutrient utilization and tissue biochemical profile of black sea bream,Acanthopagrus schlegelii,fingerlings[J].Aquaculture,2011,319(1/2):72-80.
[34] BOUJARD T,GÉLINEAU A,COVÈS D,et al.Regulation of feed intake,growth,nutrient and energy utilisation in European sea bass (Dicentrarchus labrax) fed high fat diets[J].Aquaculture,2004,231(1/2/3/4):529-545.
[35] MORAIS S,BELL J G,ROBERTSON D A,et al.Protein/lipid ratios in extruded diets for Atlantic cod (Gadus morhua L.):effects on growth,feed utilisation,muscle composition and liver histology[J].Aquaculture,2001,203(1/2):101-119.
[36] YAN J,LIAO K,WANG T J,et al.Dietary Lipid levels influence lipid deposition in the liver of large yellow croaker (Larimichthys crocea) by regulating lipoprotein receptors,fatty acid uptake and triacylglycerol synthesis and catabolism at the transcriptional level[J].PLoS One,2015,10(6):e0129937.
[37] WANG J T,LIU Y J,TIAN L X,et al.Effect of dietary lipid level on growth performance,lipid deposition,hepatic lipogenesis in juvenile cobia (Rachycentron canadum)[J].Aquaculture,2005,249(1/2/3/4):439-447.
[38] MIDTBØ L K,IBRAHIM M M,MYRMEL L S,et al.Intake of farmed atlantic salmon fed soybean oil increases insulin resistance and hepatic lipid accumulation in mice[J].PLoS One,2013,8(1):e53094.
[39] COLOMBO S.Fish nutrition and current issues in aquaculture:the balance in providing safe and nutritious seafood,in an environmentally sustainable manner[J].Journal of Aquaculture Research and Development,2014,3(3):1-10.
[40] TAN B,YIN Y L,LIU Z Q,et al.Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs[J].Amino Acids,2009,37(1):169-175.  
[41] WU L Y,FANG Y J,GUO X Y.Dietary L-arginine supplementation beneficially regulates body fat deposition of meat-type ducks[J].British Poultry Science,2011,52(2):221-226.  
[42] TAN B,YIN Y L,LIU Z Q,et al.Dietary L-arginine supplementation differentially regulates expression of lipid-metabolic genes in porcine adipose tissue and skeletal muscle[J].The Journal of Nutritional Biochemistry,2011,22(5):441-445.  
[43] SILVEIRA-COFFIGNY R,PRIETO-TRUJILLO A.Effects of different stressors in haematological variables in cultured Oreochromis aureus S[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2005,139(4):245-250.
[44] MILNER J A.Metabolic aberrations associated with arginine deficiency[J].The Journal of Nutrition,1985,115(4):516-523.  
[45] MILNER J A,PERKINS E G.Liver lipid alterations in rats fed arginine deficient diets[J].Lipids,1978,13(8):563-565.  
[46] NOGA A A,VANCE D E.A gender-specific role for phosphatidylethanolamine N-methyltransferase-derived phosphatidylcholine in the regulation of plasma high density and very low density lipoproteins in mice[J].Journal of Biological Chemistry,2003,278(24):21851-21859.  
[47] 石桂城,董晓慧,陈刚,等.饲料脂肪水平对吉富罗非鱼生长性能及其在低温应激下血清生化指标和肝脏脂肪酸组成的影响[J].动物营养学报,2012,24(11):2154-2164.
[48] FOUAD A M,EL-SENOUSEY H K,YANG X J,et al.Dietary L-arginine supplementation reduces abdominal fat content by modulating lipid metabolism in broiler chickens[J].animal,2013,7(8):1239-1245.  
[49] JOBGEN W,MEININGER C J,JOBGEN S C,et al.Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats[J].The Journal of Nutrition,2008,139(2):230-237.
[50] 刘艳妍,朱飞,邹梅,等.L-精氨酸对3-5月龄獭兔脂代谢的影响[J].饲料研究,2017(11):44-49.
[51] LIANG H L,JI K,GE X P,et al.Effects of dietary arginine on antioxidant status and immunity involved in AMPK-NO signaling pathway in juvenile blunt snout bream[J].Fish & Shellfish Immunology,2018,78:69-78.
[52] JIANG J,SHI D,ZHOU X Q,et al.In vitro and in vivo protective effect of arginine against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Fish & Shellfish Immunology,2015,42(2):457-464.  
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