卵形鲳鲹(Trachinotus ovatus)对饲料中锌的需要量

于万峰; 林黑着; 黄忠; 周婷; 虞为; 周传朋; 荀鹏伟; 黄倩倩; 黄小林

doi:10.3969/j.issn.1006-267x.2019.10.024

动物营养学报 >

2019 , Vol. 31 >Issue 10: 4602 - 4611

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.024

水产营养 Aquaculture Nutrition

卵形鲳鲹(Trachinotus ovatus)对饲料中锌的需要量

于万峰 ,
林黑着 ,
黄忠 ,
周婷 ,
虞为 ,
周传朋 ,
荀鹏伟 ,
黄倩倩 ,
黄小林

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1. 中国水产科学研究院南海水产研究所, 农业农村部南海渔业资源开发利用重点实验室, 广州 510300;
2. 上海海洋大学水产与生命学院, 上海 201306;
3. 中国水产科学研究院南海水产研究所深圳试验基地, 深圳 518121;
4. 广州大学生命科学学院, 广州 510006

于万峰(1995-),男,河南汤阴人,硕士研究生,从事动物营养与饲料科学研究。E-mail:yuwanfeng123@163.com

收稿日期: 2019-03-20

网络出版日期: 2019-10-17

基金资助

中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金项目（2017ZD01）；国家自然科学基金项目（31602175）；中国水产科学研究院基本科研业务费（2018HY-ZD0506）

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Dietary Zinc Requirement of Juvenile Golden Pompano (Trachinotus ovatus)

YU Wanfeng ,
LIN Heizhao ,
HUANG Zhong ,
ZHOU Ting ,
YU Wei ,
ZHOU Chuanpeng ,
XUN Pengwei ,
HUANG Qianqian ,
HUANG Xiaolin

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1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
3. Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China;
4. College of Life Sciences, Guangzhou University, Guangzhou 510006, China

Received date: 2019-03-20

Online published: 2019-10-17

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摘要

本试验旨在研究饲料中锌含量对卵形鲳鲹（Trachinotus ovatus）幼鱼生长性能、体成分、抗氧化能力和血清生化指标的影响，以确定卵形鲳鲹幼鱼对饲料中锌的需要量。选取平均初始体质量为（5.71±0.08）g的卵形鲳鲹幼鱼450尾，随机分成6组，每组3个重复，每个重复25尾。各组分别投喂以一水硫酸锌（ZnSO₄·H₂O）为锌源、锌含量为38.1（未添加锌组）、47.8、62.6、73.2、95.3和105.0 mg/kg的试验饲料8周。结果表明：1）62.6~105.0 mg/kg组的末体质量和增重率均显著高于未添加锌组（P<0.05），62.6~105.0 mg/kg组的饲料系数均显著低于未添加锌组（P<0.05）。2）47.8~105.0 mg/kg组的鱼体粗灰分含量显著高于未添加锌组（P<0.05）。3）62.6~105.0 mg/kg组的血清总抗氧化能力（T-AOC）均显著高于未添加锌组（P<0.05），62.6~105.0 mg/kg组的血清超氧化物歧化酶（SOD）活性显著高于未添加锌组和47.8 mg/kg组（P<0.05）。4）47.8~105.0 mg/kg组的肝脏丙二醛（MDA）含量显著低于未添加锌组（P<0.05），62.6~105.0 mg/kg组的肝脏铜锌超氧化物歧化酶（CuZn-SOD）活性显著高于未添加锌组和47.8 mg/kg组（P<0.05），73.2~105.0 mg/kg组的肝脏过氧化氢酶（CAT）活性显著高于未添加锌组（P<0.05）。5）73.2 mg/kg组的血清总蛋白（TP）含量显著高于未添加锌组（P<0.05），62.6 mg/kg组的血清白蛋白（ALB）含量显著高于未添加锌组和105.0 mg/kg组（P<0.05），47.8~105.0 mg/kg组的血清碱性磷酸酶（ALP）活性均显著高于未添加锌组（P<0.05），62.6~105.0 mg/kg组的血清高密度脂蛋白胆固醇（HDL-C）含量显著高于未添加锌组和47.8 mg/kg组（P<0.05）。由此可见，饲料中添加锌可以改善卵形鲳鲹幼鱼的生长性能和血清生化指标，提高机体的抗氧化能力。折线回归分析表明，以增重率和肝脏CuZn-SOD活性为评价指标，卵形鲳鲹幼鱼对饲料中锌的需要量分别为57.04和65.95 mg/kg。

关键词： 卵形鲳鲹; 锌; 需要量; 生长性能; 抗氧化能力

本文引用格式

于万峰 , 林黑着 , 黄忠 , 周婷 , 虞为 , 周传朋 , 荀鹏伟 , 黄倩倩 , 黄小林 . 卵形鲳鲹(Trachinotus ovatus)对饲料中锌的需要量[J]. 动物营养学报, 2019 , 31(10) : 4602 -4611 . DOI: 10.3969/j.issn.1006-267x.2019.10.024

Abstract

This study was conducted to investigate the effects of dietary zinc contents on growth performance, body composition, antioxidant capacity and serum biochemical indexes of juvenile golden pompano (Trachinotus ovatus), and to determine the zinc requirement of juvenile golden pompano. A total of 450 fish with an initial body weight of (5.71±0.08) g were randomly assigned into 6 groups with 3 replicates per group and 25 fish per replicate. The fish were fed experimental diets with zinc sulfate monohydrate (ZnSO₄·H₂O) as the zinc source for 8 weeks, which the dietary zinc contents were 38.1 (not added zinc group), 47.8, 62.6, 73.2, 95.3 and 105.0 mg/kg, respectively. The results showed as follows:1) the final body weight and weight gain rate of 62.6 to 105.0 mg/kg groups were significantly higher than those of not added zinc group (P<0.05), and the feed conversion ratio of 62.6 to 105.0 mg/kg groups was significantly lower than that of not added zinc group (P<0.05). 2) The body crude ash content of fish of 47.8 to 105.0 mg/kg groups was significantly higher than that of not added zinc group (P<0.05). 3) The serum total antioxidant capacity (T-AOC) of 62.6 to 105.0 mg/kg groups was significantly higher than that of not added zinc group (P<0.05), and the serum superoxide dismutase (SOD) activity of 62.6 to 105.0 mg/kg groups was significantly higher than that of not added zinc group and 47.8 mg/kg group (P<0.05). 4) The liver malondialdehyde (MDA) content of 47.8 to 105.0 mg/kg groups was significantly lower than that of not added zinc group (P<0.05), the liver copper, zinc-superoxide dismutase (CuZn-SOD) activity of 62.6 to 105.0 mg/kg groups was significantly higher than that of not added zinc group and 47.8 mg/kg group (P<0.05), and the liver catalase (CAT) activity of 73.2 to 105.0 mg/kg groups was significantly higher than that of not added zinc group (P<0.05). 5) The serum total protein (TP) content of 73.2 mg/kg groups was significantly higher than that of not added zinc group (P<0.05), the serum albumin (ALB) content of 62.6 mg/kg group was significantly higher than that of not added zinc group and 105.0 mg/kg groups (P<0.05), the serum alkaline phosphatase (ALP) activity of 47.8 to 105.0 mg/kg group was significantly higher than that of not added zinc group (P<0.05), the serum high density lipoprotein cholesterol (HDL-C) content of 62.6 to 105.0 mg/kg group was significantly higher than that of not added zinc group and 47.8 mg/kg group (P<0.05). In conclusion, diets added zinc can improve the growth performance and serum biochemical indexes of juvenile golden pompano, and increase the body antioxidant capacity. Broken-line regression analysis based on the weight gain rate and liver CuZn-SOD activity indicate that the dietary zinc requirements for juvenile golden pompano are 57.04 and 65.95 mg/kg, respectively.

Key words： Trachinotus ovatus; zinc; requirement; growth performance; antioxidant capacity

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