本试验旨在研究饲粮中铜添加水平对2~4月龄长毛兔生长性能、产毛性能、血清生化指标与含铜酶活性及内脏器官发育的影响。选取180只刚剪完胎毛的长毛兔，随机分为6组，每组30只，单笼饲养。6组长毛兔分别饲喂在基础饲粮中添加0（对照）、10、20、40、80及160 mg/kg铜（添加形式为五水硫酸铜）的试验饲粮，试验饲粮中铜含量的实测值依次为3.25、13.13、23.40、44.56、83.58及163.87 mg/kg，预试期7 d，正试期53 d。结果显示：饲粮铜添加水平对长毛兔的平均日增重和料重比有显著影响（P<0.05），铜添加水平为80 mg/kg时平均日增重最高，铜添加水平为40 mg/kg时料重比最低。饲粮铜添加水平对长毛兔的产毛量、皮张面积、毛直径均有显著影响（P<0.05）。铜添加水平为40 mg/kg时，皮张面积最大，产毛量最高。20、40 mg/kg组毛直径显著高于对照组（P<0.05）。饲粮铜添加水平对长毛兔血清中尿素氮（UN）含量有显著影响（P<0.05），铜添加水平为40 mg/kg时，血清中UN含量最低，为7.24 mmol/L，显著低于其他各组（P<0.05）。饲粮铜添加水平对血清总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）含量与白球比（A/G）无显著影响（P>0.05）。饲粮铜添加水平显著影响血清中铜锌超氧化物歧化酶（CuZn-SOD）和铜蓝蛋白（CP）活性（P<0.05）。40、80 mg/kg组血清CuZn-SOD活性显著高于其他各组（P<0.05）。40、80 mg/kg组血清CP活性显著高于对照组与10、20 mg/kg组（P<0.05）。饲粮铜添加水平对内脏发育指数均无显著影响（P>0.05）。根据本试验测定结果，推荐2~4月龄长毛兔饲粮中铜添加水平为40~80 mg/kg（饲粮中铜含量的实测值为44.56~83.58 mg/kg）。

This experiment was conducted to study the effects of dietary copper supplemental level on growth performance, wool performance, serum biochemical indices and copper-containing enzyme activities and visceral organ development of 2- to 4-month-old long hairy rabbits. After cutting lanugo, one hundred and eighty long hairy rabbits were randomly assigned to 6 groups with 30 rabbits in each group, the rabbits were feed separately. Rabbits in the 6 groups were fed experimental diets which supplemented with 0 (control), 10, 20, 40, 80 and 160 mg/kg copper (the form was CuSO₄·5H₂O) based on a basal diet, and the measured values of copper content in the 6 experimental diets were 3.25, 13.13, 23.40, 44.56, 83.58 and 163.87 mg/kg, respectively. The trail lasted for 7 days for adaption, and 53 days for test. The results showed as follows:dietary copper supplemental level had significant effects on average daily gain (ADG) and feed/gain (F/G) (P<0.05), the highest ADG was obtained when the copper supplemental level was 80 mg/kg, and the lowest F/G was obtained when the copper supplemental level was 40 mg/kg. Dietary copper supplemental level had significant effects on lanugo production, fur area and wool diameter of rabbits (P<0.05), the lanugo production was the highest and the fur area was the biggest when the copper supplemental level was 40 mg/kg. Dietary copper supplemental level had significant influence on serum urea nitrogen (UN) content(P<0.05), when the copper supplemental level was 40 mg/kg, the serum UN content was the lowest and it was 7.24 mmol/L, but dietary copper supplemental level had no influences on serum total protein (TP), albumin (ALB), globulin (GLB) and ALB/GLB (A/G) (P>0.05). Dietary copper supplemental level had significant influence on the activities of serum copper-zinc superoxide dismutase (CuZn-SOD) and ceruloplasmin (CP) (P<0.05). The serum CuZn-SOD activity of 40 and 80 mg/kg groups was significantly higher than that of other groups (P<0.05). The serum CP activity of 40 and 80 mg/kg groups was significantly higher than that of control group and 10, 20 mg/kg groups (P<0.05). Dietary copper supplemental level had no significant influence on organ development indices (P>0.05). Considering all results of this experiment, the appropriate copper supplemental levels are 40 to 80 mg/kg (the measured values of copper content in diets are 44.56 to 83.58 mg/kg) for 2- to 4-month-old long hairy rabbits.