动物营养学报 >

2019 , Vol. 31 >Issue 10: 4817 - 4824

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.10.047

饲料科学与技术 Feed science and technology

氯喹抑制自噬对呕吐毒素诱导的仔猪生长性能和血液指标变化的影响

  • 廖思梦 ,
  • 齐鸣 ,
  • 颜佳梦 ,
  • 李建军 ,
  • 唐宇龙 ,
  • 谭碧娥 ,
  • 印遇龙
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  • 1. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 农业部中南动物营养与饲料科学观测实验站, 长沙 410125;
    2. 中国科学院大学, 北京 100008
廖思梦(1993-),女,湖南永州人,硕士研究生,动物营养与饲料科学专业。E-mail:lsm19931@163.com

收稿日期: 2019-04-05

  网络出版日期: 2019-10-17

基金资助

国家自然科学基金项目（31672433）

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Effects of Chloroquine Inhibiting Autophagy on Growth Performance and Blood Indexes Change of Piglets Induced by Deoxynivalenol

  • LIAO Simeng ,
  • QI Ming ,
  • YAN Jiameng ,
  • LI Jianjun ,
  • TANG Yulong ,
  • TAN Bi'e ,
  • YIN Yulong
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  • 1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100008, China

Received date: 2019-04-05

  Online published: 2019-10-17

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摘要

本试验旨在探究氯喹（CQ）抑制自噬对摄入呕吐毒素（DON）污染饲粮的仔猪生长性能、血液生理生化指标和抗氧化能力的影响。选择24头初始体重[（7.10±0.58）kg]相近的健康长×大二元杂交猪，随机分为3组，分别为对照组（CON组，基础饲粮）、DON组（饲粮中含1.5 mg/kg DON）、DON＋CQ组（饲粮中含1.5 mg/kg DON＋灌服10 mg/mL CQ），每组8头，每头为1个重复。试验开始时各组均饲喂常规基础饲粮，CON和DON组灌服8 mL生理盐水；DON＋CQ组灌服8 mL CQ，试验开始后第7天，DON和DON＋CQ组饲喂含1.5 mg/kg DON的霉变饲粮，持续1周（保持CQ的灌服），试验期共14 d。结果表明：1）与CON组相比，DON组仔猪平均日增重（ADG）和平均日采食量（ADFI）显著降低（P<0.05），DON＋CQ组仔猪ADG和ADFI与其他2组均无显著差异（P>0.05），料重比（F/G）显著低于DON组（P<0.05）。2）与CON组相比，DON组血清尿素氮（UN）含量及谷丙转氨酶（ALT）和碱性磷酸酶（ALP）活性显著提高（P<0.05），DON＋CQ组血清UN含量和ALP活性显著低于DON组（P<0.05）。与CON组相比，DON组血清生长激素（GH）和胰岛素样生长因子-Ⅰ（IGF-Ⅰ）含量显著降低（P<0.05）；DON＋CQ组血清GH和IGF-Ⅰ含量显著高于DON组（P<0.05）。3）与CON组相比，DON组血浆超氧化物歧化酶（SOD）、谷胱甘肽S-转移酶（GST）、谷胱甘肽过氧化物酶（GSH-Px）活性和总抗氧化能力（T-AOC）显著降低（P<0.05），而丙二醛（MDA）含量显著升高（P<0.05）；DON+CQ组血浆SOD、GST、GSH-Px活性和T-AOC显著高于DON组（P<0.05）。结果提示，CQ抑制自噬能提高DON处理仔猪的生长性能，改善血液生化指标，提高机体抗氧化能力，一定程度上缓解DON对断奶仔猪的毒害作用。

关键词： 呕吐毒素; 仔猪; 氯喹; 血液生化指标; 抗氧化

本文引用格式

廖思梦 , 齐鸣 , 颜佳梦 , 李建军 , 唐宇龙 , 谭碧娥 , 印遇龙 . 氯喹抑制自噬对呕吐毒素诱导的仔猪生长性能和血液指标变化的影响[J]. 动物营养学报, 2019 , 31(10) : 4817 -4824 . DOI: 10.3969/j.issn.1006-267x.2019.10.047

Abstract

This experiment was conducted to study the effects of chloroquine (CQ) inhibiting autophagy on growth performance, blood physiological and biochemical indexes, and antioxidant ability of piglets fed diets contaminated by deoxynivalenol (DON). Twenty-four healthy "Long×Large" binary crossbred pigs with an initial body weight of (7.10±0.58) kg were randomly divided into 3 groups with 8 replicates per group and one pig per replicate according to a single factor complete randomized trial design. The control group (CON group) fed a basal diet, DON group was fed a DON diet (1.5 mg/kg), and DON+CQ group was fed the DON diet (1.5 mg/kg) and oral chloroquine (10 mg/mL). At the beginning of the experiment, all groups were fed the conventional basal diet, DON+CQ group was oral 8 mL chloroquine, CON and DON groups were oral administration with an equal volume of saline. On the 7 d after the beginning of the experiment, the DON and DON+CQ groups were fed mildew diet for 1 week,and the experiment lasted for 14 days. The results showed as follows:1) compared with CON group, the average daily gain (ADG) and daily feed intake (ADFI) of piglets significantly decreased (P<0.05), and there was no significant difference in ADG and ADFI between DON+CQ group and the other two groups (P>0.05), and feed/gain was significantly lower than that in DON group (P<0.05). 2) Compared with CON group, serum urea nitrogen (UN) content, glutamic-pyruvic transaminase (ALT) and alkaline phosphatase (ALP) activities in DON group significantly increased (P<0.05), and serum UN content and alkaline phosphatase (ALP) activity in DON+CQ group were significantly lower than those in DON group (P<0.05). Compared with CON group, serum growth hormone (GH) and insulin-like growth factor-Ⅰ (IGF-Ⅰ) contents significantly decreased (P<0.05), and GH and IGF-Ⅰ contents were significantly higher than those in DON group (P<0.05). 3) Compared with CON group, the activities of plasma superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in DON group significantly decreased (P<0.05), and malonaldehyde (MDA) content significantly increased (P<0.05). The activities of activities SOD, GST, GSH-Px and T-AOC in DON+CQ group were significantly higher than those in DON group (P<0.05). The results suggest that the inhibition of autophagy by CQ can improve the growth performance of piglets inhibited by DON, the blood biochemical indexes, the antioxidant capacity of the body, and alleviate the toxic effect of DON on weaned piglets to a certain extent.

Key words： deoxynivalenol; piglets; chloroquine; blood biochemical index; antioxidation

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