猪与禽营养 Swine and poultry nutrition

饲粮添加异亮氨酸对轮状病毒攻毒断奶仔猪回肠屏障功能的影响

  • 毛湘冰 ,
  • 古长松 ,
  • 陈代文 ,
  • 余冰 ,
  • 何军 ,
  • 郑萍 ,
  • 虞洁 ,
  • 罗钧秋 ,
  • 罗玉衡
展开
  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 农业农村部动物抗病营养与饲料重点实验室, 动物抗病营养四川省重点实验室, 成都 611130
毛湘冰(1980-),男,湖南长沙人,副教授,博士,研究方向为动物营养与饲料科学。E-mail:acatmxb2003@163.com

收稿日期: 2019-06-14

  网络出版日期: 2019-12-13

基金资助

现代农业产业技术体系(CARS-35);四川省教育厅重点项目(17ZA0311);四川省科技支撑项目(2016NYZ0052,2016NZ0006)

Effects of Dietary Isoleucine on Ileal Barrier Function of Weaned Piglets Challenged by Rotavirus

  • MAO Xiangbing ,
  • GU Changsong ,
  • CHEN Daiwen ,
  • YU Bing ,
  • HE Jun ,
  • ZHENG Ping ,
  • YU Jie ,
  • LUO Junqiu ,
  • LUO Yuheng
Expand
  • Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Key Laboratory of Animal Disease-Resistant Nutrition and Feed of Ministry of Agriculture and Rural Affairs, Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-06-14

  Online published: 2019-12-13

摘要

本研究旨在探讨饲粮添加异亮氨酸是否可以缓解轮状病毒(RV)感染对断奶仔猪回肠屏障功能的影响。在细胞试验中,用8 mmol/L的L-异亮氨酸处理IPEC-J2细胞24 h;在动物饲养试验中,选取28头健康的21日龄断奶的"杜×长×大"仔猪,采用2×2双因子设计,即饲粮处理(添加1%L-异亮氨酸饲粮或添加0.68%L-丙氨酸饲粮)和RV攻毒(灌服RV或无菌培养液),RV攻毒于试验第15天早上进行。试验期为18 d。结果表明,L-异亮氨酸处理提高了IPEC-J2细胞中黏液蛋白2(mucin 2)、闭锁小带蛋白-1(ZO-1)和闭合蛋白(occludin)的mRNA相对表达量(P<0.05);RV攻毒提高了断奶仔猪平均腹泻指数和血清二胺氧化酶活性(P<0.05),提高了回肠黏膜中RV的非结构蛋白4阳性率,降低了断奶仔猪回肠黏膜中mucin 2、ZO-1和occludin mRNA相对表达量(P<0.05);而饲粮添加异亮氨酸缓解了RV攻毒对断奶仔猪平均腹泻指数、血清二胺氧化酶活性和回肠黏膜mucin 2、ZO-1和occludin mRNA相对表达量的影响(P<0.05),降低了回肠黏膜中RV的非结构蛋白4阳性率。综上所述,在断奶仔猪的饲粮中补充1%异亮氨酸可以预防RV诱导发生的腹泻,而这一作用与其可改善回肠屏障功能有关。

本文引用格式

毛湘冰 , 古长松 , 陈代文 , 余冰 , 何军 , 郑萍 , 虞洁 , 罗钧秋 , 罗玉衡 . 饲粮添加异亮氨酸对轮状病毒攻毒断奶仔猪回肠屏障功能的影响[J]. 动物营养学报, 2019 , 31(12) : 5493 -5499 . DOI: 10.3969/j.issn.1006-267x.2019.12.014

Abstract

This experiment was conducted to determine whether dietary isoleucine alleviated the effects of rotavirus (RV) infection on ileal barrier function of weaned piglets. In cell culture experiment, IPEC-J2 cells were treated by 8 mmol/L L-isoleucine for 24 h. In animal breeding experiment, twenty-eight healthy crossbred (Duroc×Landrace×Yorkshire) piglets weaned at 21 days of age were used in a 2×2 factorial design. The trial factors included diet treatment (adding 1% L-isoleucine diet or adding 0.68% L-alanine diet) and RV challenge (infusing RV or sterile culture solution). On day 15, RV challenge was carried out. The trial duration was 18 days. The results showed that L-isoleucine treatment increased mRNA relative expression of mucin 2, zonula occludens-1 (ZO-1) and occludin in IPEC-J2 cells (P<0.05). RV challenge increased average diarrhea index and serum diamine oxidase activity (P<0.05), enhanced RV nonstructural protein 4 positive rate in ileal mucosa, and reduced mRNA relative expression of mucin 2, ZO-1 and occludin in ileal mucosa of weaned piglets (P<0.05). Dietary isoleucine relieved the effects of RV challenge on average diarrhea index, serum diamine oxidase activity, and mRNA relative expression of mucin 2, ZO-1 and occludin in ileal mucosa (P<0.05), and decreased RV nonstructural protein 4 positive rate in ileal mucosa of weaned piglets. These results suggest that dietary 1% isoleucine can prevent the RV-induced diarrhea, which is associated with the improvement of ileal barrier function of weaned pigs.

参考文献

[1] GU C S,MAO X B,CHEN D W,et al.Isoleucine plays an important role for maintaining immune function[J].Current Protein and Peptide Science,2019,20(7):644-651.  
[2] MAO X B,GU C S,REN M,et al.L-isoleucine administration alleviates rotavirus infection and immune response in the weaned piglet model[J].Frontiers in Immunology,2018,9:1654.
[3] 任文华,崔志洪.猪轮状病毒概述[J].畜牧兽医杂志,2005,24(5):21-25.
[4] DONELLI G,SUPERTI F.The rotavirus genus[J].Comparative Immunology Microbiology and Infectious Diseases,1994,17(3/4):305-320.
[5] BARNETT B.Viral gastroenteritis[J].Medical Clinics of North America,1983,67(5):1031-1058.  
[6] WU S P,YUAN L J,ZHANG Y G,et al.Probiotic Lactobacillus rhamnosus GG mono-association suppresses human rotavirus-induced autophagy in the gnotobiotic piglet intestine[J].Gut Pathogens,2013,5(1):22.
[7] MAO X B,QI S R N,YU B,et al.Zn2+ and L-isoleucine induce the expressions of porcine β-defensins in IPEC-J2 cells[J].Molecular Biology Reports,2013,40(2):1547-1552.  
[8] MAO X B,GU C S,HU H Y,et al.Dietary Lactobacillus rhamnosus GG supplementation improves the mucosal barrier function in the intestine of weaned piglets challenged by porcine rotavirus[J].PLoS One,2016,11(1):e0146312.
[9] MAO X B,LIU M H,TANG J,et al.Dietary leucine supplementation improves the mucin production in the jejunal mucosa of the weaned pigs challenged by porcine rotavirus[J].PLoS One,2015,10(9):e0137380.
[10] MAO X B,HU H Y,XIAO X C,et al.Lentinan administration relieves gut barrier dysfunction induced by rotavirus in a weaned piglet model[J].Food & Function,2019,10(4):2094-2101.  
[11] FRANCO D J,JOSEPHSON J K,MOEHN S,et al.Isoleucine requirement of pregnant sows[J].Journal of Animal Science,2013,91(8):3859-3866.  
[12] DONG X Y,AZZAM M M M,ZOU X T.Effects of dietary L-isoleucine on laying performance and immunomodulation of laying hens[J].Poultry Science,2016,95(10):2297-2305.  
[13] JUNTUNEN M,KIRJAVAINEN P V,OUWEHAND A C,et al.Adherence of probiotic bacteria to human intestinal mucus in healthy infants and during rotavirus infection[J].Clinical and Diagnostic Laboratory Immunology,2001,8(2):293-296.
[14] TIAN G,LIANG X F,CHEN D W,et al.Vitamin D3 supplementation alleviates rotavirus infection in pigs and IPEC-J2 cells via regulating the autophagy signaling pathway[J].The Journal of Steroid Biochemistry and Molecular Biology,2016,163:157-163.
[15] BOSHUIZEN J A,REIMERINK J H J,KORTELAND-VAN MALE A M,et al.Changes in small intestinal homeostasis,morphology,and gene expression during rotavirus infection of infant mice[J].Journal of Virology,2003,77(24):13005-13016.  
[16] HORIE Y,NAKAGOMI O,KOSHIMURA Y,et al.Diarrhea induction by rotavirus NSP4 in the homologous mouse model system[J].Virology,1999,262(2):398-407.  
[17] MAO X B,ZENG X F,QIAO S Y,et al.Specific roles of threonine in intestinal mucosal integrity and barrier function[J].Frontiers in Bioscience (Elite Edition),2011,3(4):1192-1200.
[18] DIAO H,YAN H L,XIAO Y,et al.Modulation of intestine development by fecal microbiota transplantation in suckling pigs[J].RSC Advances,2018,8(16):8709-8720.  
[19] LAUKOETTER M G,BRUEWER M,NUSRAT A.Regulation of the intestinal epithelial barrier by the apical junctional complex[J].Current Opinion in Gastroenterology,2006,22(2):85-89.  
[20] DEPLANCKE B,GASKINS H R.Microbial modulation of innate defense:goblet cells and the intestinal mucus layer[J].The American Journal of Clinical Nutrition,2001,73(6):1131S-1141S.
文章导航

/