动物营养学报 >

2019 , Vol. 31 >Issue 12: 5613 - 5624

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.027

反刍与草食动物营养 Ruminant and herbivore nutrition

补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马3 600 m速度赛赛前赛后血浆中代谢物的影响

  • 张仕琦 ,
  • 李晓斌 ,
  • 张文杰 ,
  • 韩明 ,
  • 王世昌 ,
  • 杨开伦
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  • 新疆农业大学动物科学学院, 新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052
张仕琦(1993-),男,新疆额敏人,博士研究生,研究方向草食动物营养代谢。E-mail:1505521966@qq.com

收稿日期: 2019-06-13

  网络出版日期: 2019-12-13

基金资助

自治区“十三五”重大专项“运动马健康养殖共性技术研发”（2017A01002-2）

收起

Effects of Supplemented with L-Arginine or N-Carbamylglutamate on Plasma Metabolites before and after 3 600 m Speed Race of Yili Horses

  • ZHANG Shiqi ,
  • LI Xiaobin ,
  • ZHANG Wenjie ,
  • HAN Ming ,
  • WANG Shichang ,
  • YANG Kailun
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  • Xinjiang Key Laboratory of Meat&Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2019-06-13

  Online published: 2019-12-13

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摘要

本试验旨在探究补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马3 600 m速度赛赛前赛后血浆中代谢物的影响。选取15匹年龄为2岁、体重[（388.88±23.24） kg]相近、经过严格训练的速度赛用伊犁马，分为3组，每组5匹（3♂+2♀），每日进行等强度训练，对照组补喂123 g/（匹·d）L-丙氨酸，L-精氨酸组补喂60 g/（匹·d）L-精氨酸，N-氨甲酰谷氨酸组补喂12 g/（匹·d） N-氨甲酰谷氨酸和112 g/（匹·d）L-丙氨酸，在试验第28天进行3 600 m速度赛，采集赛前2 h、赛后即刻和赛后2 h血液样品，利用液相色谱-质谱（LC-MS）分析技术测定血浆中代谢物。结果显示：与对照组相比，L-精氨酸组伊犁马赛前2 h血浆柠檬酸、赛后即刻血浆α-酮己二酸、赛后2 h血浆D-葡萄糖-6-磷酸含量显著升高（P<0.05），赛后即刻血浆乳酸含量显著降低（P<0.05）；N-氨甲酰谷氨酸组赛后即刻血浆乳酸含量显著降低（P<0.05）。与对照组相比，L-精氨酸组赛前2 h血浆精氨酸、乙酰谷氨酸、谷氨酰脯氨酸含量显著升高（P<0.05），血浆谷氨酰胺含量显著降低（P<0.05），赛后即刻血浆鸟氨酸、乙酰谷氨酸和脯氨酰胺含量显著升高（P<0.05）；N-氨甲酰谷氨酸组赛前2 h血浆精氨酸含量显著升高（P<0.05）。此外，与对照组相比，L-精氨酸组、N-氨甲酰谷氨酸组赛前2 h血浆亮氨酸、色氨酸、5-羟色氨酸含量显著升高（P<0.05）。本试验应用非靶向代谢组学技术，筛选出了补喂L-精氨酸或N-氨甲酰谷氨酸后伊犁马3 600 m速度赛赛前赛后血浆中差异性表达代谢物，揭示出补喂L-精氨酸或N-氨甲酰谷氨酸能够促进伊犁马运动能量代谢，影响氨基酸代谢，促进精氨酸内源性合成和精氨酸向脯氨酸的代谢。

关键词： 伊犁马; L-精氨酸; N-氨甲酰谷氨酸; 非靶向代谢组学; 血浆代谢物

本文引用格式

张仕琦 , 李晓斌 , 张文杰 , 韩明 , 王世昌 , 杨开伦 . 补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马3 600 m速度赛赛前赛后血浆中代谢物的影响[J]. 动物营养学报, 2019 , 31(12) : 5613 -5624 . DOI: 10.3969/j.issn.1006-267x.2019.12.027

Abstract

The aim of this study was to investigate the effects of supplemented with L-arginine (L-Arg) or N-carbamylglutamate (NCG) on plasma metabolites before and after of 3 600 m speed race of Yili horses. Fifteen Yili horses with similar body weight[(388.88±23.24) kg], aged 2 years old, were divided into 3 groups (5 horses in each group, 3♂+2♀) and trained according to the same training programs. Horses in control group were supplemented with 123 g/(horse·d) L-alanine (L-Ala), those in L-Arg group were supplemented with 60 g/(horse·d) L-Arg, and those in NCG group were supplemented with 12 g/(horse·d) NCG and 112 g/(horse·d). The experiment lasted for 4 weeks. The 3 600 m speed race was conducted at the 28th day of experiment, and samples were collected at pre-race 2 h, immediately post-race and post-race 2 h to analysis plasma metabolites by liquid chromatograph/mass spectrometer (LC/MS) technology. The results showed that, compared with the control group, the contents of plasma citric acid at pre-race 2 h, plasma α-ketoadipic acid at immediately post-race, and plasma D-glucose-6-phosphate at post-race 2 h in L-Arg group were significantly increased (P<0.05), and the content of plasma lactic acid was significantly decreased at immediately post-race (P<0.05); the content of plasma in NCG group was significantly decreased at immediately post-race (P<0.05). Compared with the control group, the contents of plasma arginine, acetylglutamic acid, glutamyl proline were significantly increased (P<0.05) and plasma glutamine content was significantly decreased in L-Arg group at pre-race 2 h (P<0.05), and the contents of plasma ornithine, acetylglutamic acid and proline were significantly increased at immediately post-race (P<0.05); the content of plasma arginine in NCG group was significantly increased at pre-race 2 h (P<0.05). Moreover, the contents of plasma leucine, tryptophan and 5-hydroxytryptophan in L-Arg group and NCG group were significantly increased at pre-race 2 h (P<0.05). In this experiment, the plasma differential expression metabolites before and after 3 600 m speed race of Yili horses supplemented with L-Arg or N-carbamylglutamic acid are screened by non-targeted metabonomics. It is revealed that supplemented with L-Arg or NCG can promote exercise energy metabolism, affect amino acid metabolism, promote the synthesis of arginine and the metabolism of arginine to proline in Yili horses.

Key words： Yili horses; L-arginine; N-carbamylglutamate; non-targeted metabolomics; plasma metabolites

参考文献

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