动物营养学报 >

2019 , Vol. 31 >Issue 12: 5625 - 5634

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.028

水产营养 Aquaculture Nutrition

饲料添加N-氨甲酰谷氨酸对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响

  • 赵红霞 ,
  • 乔国贤 ,
  • 黄燕华 ,
  • 曹俊明 ,
  • 陈冰 ,
  • 王国霞 ,
  • 陈晓瑛
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  • 1. 广东省农业科学院动物科学研究所, 广州 510640;
    2. 农业部华南动物营养与饲料重点实验室, 广州 510640;
    3. 广东省畜禽育种与营养研究重点实验室, 广州 510640
赵红霞(1976-),女,内蒙古乌海人,研究员,博士,从事水产动物营养与饲料的研究。E-mail:zhaohongxia8866@163.com

收稿日期: 2019-06-17

  网络出版日期: 2019-12-13

基金资助

国家自然科学基金项目（31402307）；省级现代农业产业技术推广体系建设项目（2018LM1082，2018LM1083）；广东省农业科学院学科团队建设（201614TD）；广东省科技计划项目（2019A050505007）

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Effects of Dietary N-Carbamylglutamate on Growth Performance, Body Composition, Serum Biochemical Indices and Anti-Ammonia- Nitrogen Stress Ability of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

  • ZHAO Hongxia ,
  • QIAO Guoxian ,
  • HUANG Yanhua ,
  • CAO Junming ,
  • CHEN Bing ,
  • WANG Guoxia ,
  • CHEN Xiaoying
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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Guangzhou 510640, China;
    3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2019-06-17

  Online published: 2019-12-13

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摘要

本试验旨在研究饲料中添加N-氨甲酰谷氨酸（NCG）对黄颡鱼（Pelteobagrus fulvidraco）幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响。选取初始体重为（0.98±0.03） g的黄颡鱼800尾，随机分为5组，每组设置4个重复，分别投喂添加0、250、500、1 000、2 000 mg/kg NCG的5种等氮等脂（42%粗蛋白质和7%脂肪）试验饲料。饲养56 d后，应用氯化铵进行72 h氨氮应激试验。结果表明：1）特定生长率在250 mg/kg组达到最大值，显著高于其他各组（P<0.05）；与0 mg/kg组相比，NCG添加量达到2 000 mg/kg时黄颡鱼饲料系数显著升高（P<0.05），存活率显著下降（P<0.05）。2）500、1 000和2 000 mg/kg组黄颡鱼血清总蛋白含量显著高于0 mg/kg组（P<0.05）。黄颡鱼的肝体比、脏体比及血清甘油三酯、葡萄糖、高密度脂蛋白含量在2 000 mg/kg组达到最低，血清尿素含量在2 000 mg/kg组达到最高。饲料中添加NCG对黄颡鱼肥满度，全鱼干物质、粗蛋白质、粗脂肪、粗灰分含量，血清低密度脂蛋白含量及血清谷丙转氨酶、谷草转氨酶活性无显著影响（P>0.05）。3）黄颡鱼氨氮应激24、48、72 h的累积死亡率随饲料NCG添加量增加均呈现先降低后升高趋势，在250 mg/kg组累积死亡率达到最低。综上所述，饲料添加250 mg/kg NCG能够提高黄颡鱼幼鱼的生长性能和抗氨氮应激能力，添加2 000 mg/kg NCG降低黄颡鱼幼鱼的特定生长率、饲料利用率和存活率。以特定生长率为评价指标，通过二次回归分析计算出黄颡鱼幼鱼饲料中NCG的适宜添加量为217.98 mg/kg。

关键词： N-氨甲酰谷氨酸; 黄颡鱼; 生长性能; 血清生化指标; 氨氮应激

本文引用格式

赵红霞 , 乔国贤 , 黄燕华 , 曹俊明 , 陈冰 , 王国霞 , 陈晓瑛 . 饲料添加N-氨甲酰谷氨酸对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响[J]. 动物营养学报, 2019 , 31(12) : 5625 -5634 . DOI: 10.3969/j.issn.1006-267x.2019.12.028

Abstract

This experiment was conducted to investigate the effects of dietary N-carbamylglutamate (NCG) on growth performance, body composition, serum biochemical indices and anti-ammonia-nitrogen stress ability of juvenile yellow catfish (Pelteobagrus fulvidraco). Five isonitrogenous and isolipidic diets (42% crude protein and 7% lipid) were formulated to contain graded levels of NCG (0, 250, 500, 1 000 and 2 000 mg/kg). Eight hundred juvenile yellow catfish with an initial average body weight of (0.98±0.03) g were randomly divided into 5 groups with 4 replicates of 40 fish each and each group was fed one of the diets. After 56 d feeding, fish were exposed to ammonia-nitrogen for 72 h. Results showed as follows:1) specific growth rate (SGR) in 250 mg/kg group was the highest, and significantly higher than that in other groups (P<0.05). The feed conversion rate (FCR) in 2 000 mg/kg group increased significantly (P<0.05), and the survival rate in 2 000 mg/kg group significantly decreased when compared with other groups (P<0.05). 2) The serum total protein content in 500, 1 000 and 2 000 mg/kg groups was significantly higher than that in 0 mg/kg group (P<0.05). The hepatosomatic index and viscerosomatic index and serum triglycerides, glucose, and high-density lipoprotein contents in 2 000 mg/kg group were the lowest, serum urea content in 2 000 mg/kg group was the highest. Dietary NCG had no significant effect on condition factor, whole-body dry matter, crude protein, crude lipid, ash contents, as well as serum low density lipoprotein content, aspartate aminotransferase and alanine aminotransferase activities (P>0.05). 3) After 24, 48 and 72 h challenge to ammonia-nitrogen stress, cumulative mortality rate was decreased firstly and then increased with dietary NCG additive amount increasing, and minimum cumulative mortality rate was observed in 250 mg/kg group. In conclusion, dietary 250 mg/kg NCG can improve growth performance and anti-ammonia-nitrogen stress ability of juvenile yellow catfish and a level of 2 000 mg/kg NCG seem to depress the specific growth rate, feed utilization and survival rate. With specific growth rate as evaluation index, the optimal dietary NCG additive amount is estimated to be 217.98 mg/kg using a quadratic regression analysis.

Key words： N-carbamylglutamate; Pelteobagrus fulvidraco; growth performance; serum biochemical indices; ammonia-nitrogen stress

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