动物营养学报 >

2019 , Vol. 31 >Issue 12: 5645 - 5654

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.030

水产营养 Aquaculture Nutrition

饲料蛋白质水平对拉萨裸裂尻鱼幼鱼免疫和抗氧化能力的影响

  • 徐兆利 ,
  • 曾本和 ,
  • 杨瑞斌 ,
  • 牟振波 ,
  • 李宝海 ,
  • 王万良 ,
  • 张忭忭 ,
  • 刘海平
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  • 1. 华中农业大学水产学院, 武汉 430070;
    2. 西藏自治区农牧科学院水产科学研究所, 拉萨 850000;
    3. 西藏自治区农牧科学院, 拉萨 850000
徐兆利(1994-),男,云南宣威人,硕士研究生,从事水产动物营养与饲料研究。E-mail:zhaolixu@163.com

收稿日期: 2019-06-13

  网络出版日期: 2019-12-13

基金资助

西藏财政专项（XZNKY-2018-C-040，2017CZZX004，2017CZZX003）；西藏自治区自然科学基金（ZRKX2017000143）；西藏财政厅农业科技服务资金（2018SFTG01）；西藏科技厅重点研发及转化计划（XZ201801NB12）

收起

Effects of Dietary Protein Level on Immune and Antioxidant Capacity of Juvenile Schizopygopsis younghusbandi Regan

  • XU Zhaoli ,
  • ZENG Benhe ,
  • YANG Ruibin ,
  • MOU Zhenbo ,
  • LI Baohai ,
  • WANG Wanliang ,
  • ZHANG Bianbian ,
  • LIU Haiping
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  • 1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China;
    3. Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China

Received date: 2019-06-13

  Online published: 2019-12-13

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摘要

本试验旨在研究饲料蛋白质水平对拉萨裸裂尻鱼（Schizopygopsis youughusbaudi）幼鱼免疫和抗氧化能力的影响。选择健康、大小均匀、已驯化完全、体重（22.42±0.56） g的拉萨裸裂尻鱼幼鱼540尾，随机分成6组，每组3个重复，每个重复30尾。各组试验鱼分别投喂蛋白质水平为20%、25%、30%、35%、40%、45%的等脂等能饲料。试验期60 d。结果表明：1）30%、35%蛋白质水平组的体重增长率显著高于其他各组（P<0.05）。2）饲料蛋白质水平对拉萨裸裂尻鱼幼鱼的血清和肝脏的免疫和抗氧化指标有显著影响（P<0.05）。血清溶菌酶（LSZ）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和免疫球蛋白M （IgM）含量以及肝脏ACP、AKP、SOD、CAT活性均随饲料蛋白质水平的增加呈先升高后降低的趋势，肝脏丙二醛（MDA）含量随饲料蛋白质水平的增加呈先降低后趋于稳定的趋势。其中，血清或肝脏免疫酶LZM、ACP、AKP活性和抗氧化酶CAT、SOD活性及血清IgM含量均在饲料蛋白质水平为30%或35%时最高，肝脏MDA含量在饲料蛋白质水平为35%之后趋于稳定。综合考虑生长、免疫和抗氧化指标，经多项式回归分析得出，（22.42±0.56） g拉萨裸裂尻鱼幼鱼人工配合饲料的适宜蛋白质水平为30.43%~33.88%。

关键词： 拉萨裸裂尻鱼; 蛋白质; 免疫; 抗氧化

本文引用格式

徐兆利 , 曾本和 , 杨瑞斌 , 牟振波 , 李宝海 , 王万良 , 张忭忭 , 刘海平 . 饲料蛋白质水平对拉萨裸裂尻鱼幼鱼免疫和抗氧化能力的影响[J]. 动物营养学报, 2019 , 31(12) : 5645 -5654 . DOI: 10.3969/j.issn.1006-267x.2019.12.030

Abstract

This experiment was conducted to study the effects of dietary protein level on immune and antioxidant capacity of juvenile Schizopygopsis youughusbaudi Regan. A total of 540 healthy, uniform-sized, fully domesticated juvenile Schizopygopsis youughusbaudi Regan with body weight of (22.42±0.56) g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in each group were fed experimental diets that the protein levels were 20%, 25%, 30%, 35%, 40% and 45%, respectively. The experiment lasted for 60 days. The results showed as follows:1) the body weight growth rate of 30% and 35% protein level groups was significantly higher than that of other groups (P<0.05). 2) Dietary protein level had significant effects on the indexes of immune and antioxidant in serum and liver of juvenile Schizopygopsis youughusbaudi Regan (P<0.05). The lysozyme (LSZ), acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) activities and immunoglobulin M (IgM) content in serum and activities of ACP, AKP, SOD and CAT in liver were showed a firstly increased and then decreased trend with dietary protein level increased, and the liver malondialdehyde (MDA) content was showed a firstly decreased and then stabilize trend with dietary protein level increased. The activities of immune enzymes LZM, ACP, AKP and antioxidant enzymes CAT, SOD in serum or liver and serum IgM content were the highest at 30% or 35% of dietary protein level, and the liver MDA content stabled after 35% of dietary protein level. In conclusion, considering the growth, immunity and antioxidant indexes, the polymorphic regression analysis shows that the suitable protein level of the artificial compound feed of (22.42±0.56) g juvenile Schizopygopsis youughusbaudi Regan is 30.43% to 33.88%.

Key words： Shizopygopsis younghusbandi Regan; protein; immunity; antioxidant

参考文献

[1] 武云飞,吴翠珍.青藏高原鱼类[M].成都:四川科技出版社,1991:7.
[2] 杨汉运,黄道明,谢山,等.雅鲁藏布江中游渔业资源现状研究[J].水生态学杂志,2010,3(6):120-126.
[3] 朱挺兵,陈亮,杨德国,等.雅鲁藏布江中游裂腹鱼类的分布及栖息地特征[J].生态学杂志,2017,36(10):2817-2823.
[4] 季强.六种裂腹鱼类摄食消化器官形态学与食性的研究[D].硕士学位论文.武汉:华中农业大学,2008.
[5] 许静.雅鲁藏布江四种特有裂腹鱼类早期发育的研究[D].硕士学位论文.武汉:华中农业大学,2011.
[6] 杨学峰.拉萨裸裂尻鱼的食性及食物选择的研究[D].硕士学位论文.武汉:华中农业大学,2011.
[7] 段友健.拉萨裸裂尻鱼个体生物学和种群动态研究[D].博士学位论文.武汉:华中农业大学,2015.
[8] 旦增旺久,强巴央宗,刘海平,等.西藏裂腹鱼类出血病病原的初步研究[J].水生态学杂志,2012,33(6):124-127.
[9] 李文祥,张立强,高谦,等.西藏拉萨河鱼类内寄生蠕虫的种类组成及其群落特征[J].动物学杂志,2008,43(2):1-8.
[10] 洛桑,蒋海,旦增达瓦,等.3种拉萨河鱼类肌肉脂肪酸的组成分析[J].食品与营养科学,2013,2(1):12-15.
[11] 曾本和,张忭忭,刘海平,等.饲料蛋白质水平对拉萨裸裂尻鱼幼鱼生长、饲料利用、形体指标和肌肉营养成分的影响[J].动物营养学报,2019,31(3):1231-1239.
[12] 艾庆辉,麦康森.鱼类营养免疫研究进展[J].水生生物学报,2007,31(3):425-430.
[13] WU G Y,FLYNN N E,FLYNN S P,et al.Dietary protein or arginine deficiency impairs constitutive and inducible nitric oxide synthesis by young rats[J].The Journal of Nutrition,1999,129(7):1347-1354.  
[14] 汪益峰,周维仁,章世元,等.氨基酸平衡和外源酶对异育银鲫的生长、氮代谢及血液生化指标的影响[J].江苏农业学报,2010,26(1):130-135.
[15] CATACUTAN M R,COLOSO R M.Effect of dietary protein to energy ratios on growth,survival,and body composition of juvenile Asian seabass,Lates calcarifer[J].Aquaculture,1995,131(1/2):125-133.
[16] TIBBETTS S M,LALL S P,ANDERSON D M.Dietary protein requirement of juvenile American eel (Anguilla rostrata) fed practical diets[J].Aquaculture,2000,186(1/2):145-155.
[17] CHO C Y,COWEY C B,WATANABE T.Methodological approaches to research and development[M].Ottawa:IDRC,1985:10-80.
[18] SANTINHA P J M,GOMES E F S,COIMBRA J O.Effects of protein level of the diet on digestibility and growth of gilthead sea bream,Sparus auratus L.[J].Aquaculture Nutrition,1996,2(2):81-87.  
[19] KIM K W,WANG X J,BAI S C.Optimum dietary protein level for maximum growth of juvenile olive flounder Paralichthys olivaceus (Temminck et Schlegel)[J].Aquaculture Research,2002,33(9):673-679.  
[20] 孙金辉,范泽,张美静,等.饲料蛋白水平对鲤幼鱼肝功能和抗氧化能力的影响[J].南方水产科学,2017,13(3):113-119.
[21] 蒋阳阳.不同蛋白质、脂肪和糖水平对团头鲂幼鱼生长性能、体组成和生理生化指标的影响[D].硕士学位论文.南京:南京农业大学,2012.
[22] 鲁琼,王立改,楼宝,等.饲料蛋白质水平对黄姑鱼幼鱼生长性能、体组成和消化酶活性的影响[J].动物营养学报,2015,27(12):3763-3771.
[23] 孙瑞健,张文兵,徐玮,等.饲料蛋白质水平与投喂频率对大黄鱼生长、体组成及蛋白质代谢的影响[J].水生生物学报,2013,37(2):281-289.
[24] 曾本和,王万良,朱龙,等.饲料蛋白质水平对台湾泥鳅生长性能、形体指标和体成分的影响[J].动物营养学报,2017,29(9):3413-3421.
[25] 徐起群.尼罗罗非鱼和奥尼罗非鱼幼鱼蛋白质营养需求研究[D].硕士学位论文.南京:南京农业大学,2012.
[26] 肖温温.饲料中脂肪与蛋白质水平对大口黑鲈生长、体组成、非特异性免疫和血液学的影响[D].硕士学位论文.上海:上海海洋大学,2012.
[27] 杨俊江.三个生长阶段斜带石斑鱼蛋白质、脂肪和碳水化合物需要量研究[D].硕士学位论文.湛江:广东海洋大学,2013.
[28] 唐媛媛.饲料蛋白质水平对卵形鲳鲹消化酶、非特异性免疫和肠道内容物细菌的影响[D].硕士学位论文.厦门:厦门大学,2014.
[29] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1/2):248-254.
[30] BARTON B A,IWAMA G K.Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of Corticosteroids[J].Annual Review of Fish Diseases,1991,1:3-26.
[31] HUANG J F,XU Q Y,CHANG Y M.Effects of temperature and dietary protein on gene expression of Hsp70 and Wap65 and immunity of juvenile mirror carp (Cyprinus carpio)[J].Aquaculture Research,2015,46(11):2776-2788.  
[32] 强俊,杨弘,王辉,等.饲料蛋白水平对低温应激下吉富罗非鱼血清生化指标和HSP70 mRNA表达的影响[J].水生生物学报,2013,37(3):434-443.
[33] 张宝龙,高木珍,程镇燕,等.降低饲料蛋白水平对鲤鱼生长、体成分及免疫力的影响[J].饲料研究,2015(8):49-55.
[34] 王常安,徐奇友,唐玲,等.大鳞鲃幼鱼蛋白质的需求量[J].华中农业大学学报,2014,33(3):90-96.
[35] 乐贻荣,杨弘,徐起群,等.饲料蛋白水平对奥尼罗非鱼(Oreochromis niloticus×O. aureus)生长、免疫功能以及抗病力的影响[J].海洋与湖沼,2013,44(2):493-498.
[36] 朱仙龙.不同蛋白、脂肪水平饲料循环投喂对两种石斑鱼生长及饲料利用的影响[D].硕士学位论文.海口:海南大学,2014.
[37] 谭青松.异育银鲫和长吻鮠对饲料碳水化合物利用的比较研究[D].博士学位论文.武汉:中国科学院研究生院,2005.
[38] 华颖.黑鲷幼鱼对饲料中碳水化合物利用及其机理研究[D].博士学位论文.杭州:浙江大学,2017.
[39] 任鸣春.军曹鱼和虹鳟糖类营养生理研究[D].博士学位论文.青岛:中国海洋大学,2012.
[40] 王芬.饲料中高淀粉含量对异育银鲫生长、代谢及鱼体成分的影响[D].硕士学位论文.武汉:华中农业大学,2008.
[41] DENG D F,REFSTIE S,HEMRE G I,et al.A new technique of feeding,repeated sampling of blood and continuous collection of urine in white sturgeon[J].Fish Physiology and Biochemistry,2000,22(3):191-197.  
[42] TENGJAROENKUL B,SMITH B J,CACECI T,et al.Distribution of intestinal enzyme activities along the intestinal tract of cultured Nile tilapia,Oreochromis niloticus L[J].Aquaculture,2000,182(3/4):317-327.
[43] 孙静秋,许燕,张慧绮,等.凡纳对虾体内ACP、AKP酶的细胞化学定位[J].复旦学报(自然科学版),2007,46(6):947-951.
[44] KANAK E G,DOGAN Z,EROGLU A,et al.Effects of fish size on the response of antioxidant systems of Oreochromis niloticus following metal exposures[J].Fish Physiology and Biochemistry,2014,40(4):1083-1091.
[45] VIARENGO A,CANESI L,MARTINEZ P G,et al.Pro-oxidant processes and antioxidant defence systems in the tissues of the Antarctic scallop (Adamussium colbecki) compared with the Mediterranean scallop (Pecten jacobaeus)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1995,111(1):119-126.  
[46] 张晨捷,彭士明,陈超.饲料蛋白和脂肪水平对云纹石斑鱼幼鱼免疫和抗氧化性能的影响[J].海洋渔业,2016,38(6):634-644.
[47] 张宜奎,宋秀凯,刘爱英,等.Cd2+胁迫对文蛤鳃组织SOD、CAT活性及MDA含量的影响[J].安全与环境学报,2011,11(6):19-23.
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