复合植物提取物对奶牛生产性能及血清免疫、抗氧化指标的影响

徐腾腾; 张腾龙; 王丽芳; 吕可欣; 敖长金

doi:10.3969/j.issn.1006-267x.2019.12.037

动物营养学报 >

2019 , Vol. 31 >Issue 12: 5707 - 5718

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.037

饲料科学与技术 Feed science and technology

复合植物提取物对奶牛生产性能及血清免疫、抗氧化指标的影响

徐腾腾 ,
张腾龙 ,
王丽芳 ,
吕可欣 ,
敖长金

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1. 内蒙古自治区农牧业科学院, 呼和浩特 010031;
2. 内蒙古农业大学动物科学学院, 呼和浩特 010018

徐腾腾(1993-),内蒙古通辽人,硕士研究生,研究方向为反刍动物营养与饲料。E-mail:13190601497@163.com

收稿日期: 2019-05-24

网络出版日期: 2019-12-13

基金资助

内蒙古农牧业科学院创新基金（2017CXJJM09）；内蒙古科技成果转化项目（CGZH2018159）

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Effects of Compound Plant Extracts on Production, Serum Immune and Antioxidant Indexes of Dairy Cows

XU Tengteng ,
ZHANG Tenglong ,
WANG Lifang ,
LYU Kexin ,
AO Changjin

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1. Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Autonomous Region, Hohhot 010031, China;
2. College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2019-05-24

Online published: 2019-12-13

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摘要

本试验旨在研究复合植物提取物对奶牛生产性能及血清免疫、抗氧化指标的影响。以金银花、蒲公英、益母草、连翘4种全株植物乙醇提取物按重量比1：1：1：1混合制成复合植物提取物。采用完全随机试验设计，选取胎次、泌乳期、泌乳量及体况相近的24头荷斯坦奶牛，随机分为2组，每组12头。对照组饲喂基础饲粮，试验组饲喂基础饲粮+54 g/（头·d）复合植物提取物。预试期15 d，正试期60 d。结果表明：1）饲粮中添加复合植物提取物对奶牛产奶量、干物质采食量、营养物质表观消化率没有显著影响（P>0.05）。试验组第60天乳蛋白率显著高于对照组（P<0.05），试验组第30天体细胞数显著低于对照组（P<0.05）。2）与对照组相比，试验组第30天、第45天和第60天血清总蛋白含量显著增加（P<0.05），血清白蛋白含量显著降低（P<0.05）；试验组第30天和第45天血清免疫球蛋白A含量显著增加（P<0.05），试验组第30天血清免疫球蛋白M含量显著降低（P<0.05），试验组第15天血清免疫球蛋白G含量显著降低（P<0.05），试验组各时间点血清白细胞介素-2含量显著降低（P<0.05）。3）与对照组相比，试验组第30天血清谷胱甘肽过氧化物酶活性显著升高（P<0.05），试验组第15天和第45天血清超氧化物歧化酶活性显著升高（P<0.05），试验组第15天和第60天血清总抗氧化能力显著提高（P<0.05），试验组第30天和第60天血清一氧化氮含量和一氧化氮合成酶活性均显著提高（P<0.05）。由此可见，饲粮中添加复合植物提取物对奶牛产奶量、干物质采食量和营养物质表观消化率没有显著影响，但对机体的免疫功能及抗氧化能力有的促进效果。

关键词： 复合植物提取物; 奶牛; 生产性能; 免疫; 抗氧化

本文引用格式

徐腾腾 , 张腾龙 , 王丽芳 , 吕可欣 , 敖长金 . 复合植物提取物对奶牛生产性能及血清免疫、抗氧化指标的影响[J]. 动物营养学报, 2019 , 31(12) : 5707 -5718 . DOI: 10.3969/j.issn.1006-267x.2019.12.037

Abstract

The purpose of this experiment was to study the effects of compound plant extracts on production, serum immune and antioxidant indexes of dairy cows. Four kinds of whole plant ethanol extracts of honeysuckle, dandelion, motherwort and forsythia suspense were mixed by weight ratio of 1:1:1:1 to make the compound plant extracts. A complete random experiment design was adopted in this experiment, twenty-four Holstein cows with similar parity, lactation period, milk yield and body condition were randomly divided into 2 groups with 12 cows per group. The control group was fed a basic diet, and the experimental group was fed the basic diet+54 g/(head·d) compound plant extracts. The pre-experimental period lasted for 15 days, and the experimental period lasted for 60 days. The results showed as follows:1) dietary supplementation of compound plant extracts had no significant effects on milk yield, dry matter intake and nutrient apparent digestibility of dairy cows (P>0.05). The milk protein percentage at day 60 of experimental group was significantly higher than that of the control group (P<0.05), and the somatic cell count at day 30 of experimental group was significantly lower than that of the control group (P<0.05). 2) Compared with the control group, the serum total protein content at days 30, 45 and 60 of experimental group was significantly increased (P<0.05), and the serum albumin content was significantly decreased (P<0.05); the serum immunoglobulin A content at days 30 and 45 of experimental group was significantly increased (P<0.05), the serum immunoglobulin M content at day 30 of experimental group was significantly decreased (P<0.05), the serum immunoglobulin G content at day 15 of experimental group was significantly decreased (P<0.05), and the serum interleukin-2 content at each time point of experimental group was significantly decreased (P<0.05). 3) Compared with the control group, the serum glutathione peroxidase activity at day 30 of experimental group was significantly increased (P<0.05), the serum superoxide dismutase activity at days 15 and 45 of experimental group was significantly increased (P<0.05), the serum total antioxidant capacity at days 15 and 60 of experimental group was significantly increased (P<0.05), and the nitric oxide content and nitric oxide synthase activity in serum at days 30 and 60 of experimental group were significantly increased (P<0.05). In conclusion, supplementation of compound plant extracts has no significant effects on milk yield, dry matter intake and nutrient apparent digestibility of dairy cows, but has promotion effects on body immune function and antioxidant capacity.

Key words： compound plant extracts; dairy cows; performance; immune; antioxidant

参考文献

[1] 张然,郑琛,闫晓刚,等.植物提取物对反刍动物瘤胃发酵和甲烷排放影响的研究进展[J].东北农业科学,2017,42(5):43-47.
[2] 张华,童津津,孙铭维,等.植物提取物对反刍动物瘤胃发酵、生产性能及甲烷产量的调控作用及其机制[J].动物营养学报,2018,30(6):2027-2035.
[3] 李若鹏.植物提取物行业现状与发展[J].管理观察,2018(2):175-176.
[4] 韦松叶,郑宏伟,韦海芬,等.荷斯坦奶牛优质高产集成技术研究与应用[J].广西畜牧兽医,2018,34(6):307-308.
[5] 徐哲,周凌云,贾亚雄,等.提高中国奶业科技国际地位的战略思考[J].世界农业,2018(8):62-65.
[6] 胡永灵,叶世莉,罗佳捷.中草药制剂对热应激奶牛泌乳性能、抗氧化能力及免疫功能的影响[J].草业学报,2015,24(1):132-140.
[7] 常肖肖,张议夫,赵氏萍,等.茶皂素对奶牛免疫功能的影响[J].动物营养学报,2017,29(3):1039-1045.
[8] 赵国玲,刘佳佳,林丹,等.金银花化学成分及药理研究进展[J].中成药,2002,24(12):973-976.
[9] 唐志文,蒋林树,杨亮,等.金银花提取物在围产期奶牛氧化应激及炎症中的应用前景[J].动物营养学报,2018,30(10):3843-3849.
[10] 唐志文.金银花提取物对围产期奶牛健康状况及生产性能的作用效果研究[D].硕士学位论文,北京:中国农业科学院,2018.
[11] 洪金锁,闵继光,刘建雷.中草药添加剂(蒲公英流浸膏)对奶牛乳房炎及其生产性能的影响[J].当代畜牧,2018(6):13-17.
[12] HU C,KITTS D D.Antioxidant,prooxidant,and cytotoxic activities of solvent-fractionated dandelion (Taraxacum officinale) flower extracts in vitro[J].Journal of Agricultural & Food Chemistry,2003,51(1):301-310.

[13] 周艳,张涛,郑勤琴,等.连翘有效成分提取及提取物对奶牛乳房炎致病菌的抑制效果[J].生物技术进展,2014,4(4):292-295,305.
[14] 安胜英,刘观忠,景大明,等.三种植物提取物对蛋鸡初产性能的影响[J].黑龙江畜牧兽医,2018(4):162-164.
[15] 王加启.现代奶牛养殖科学[M].北京:中国农业出版社,2006,415-416.
[16] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 23742-2009饲料中盐酸不溶灰分的测定[S].北京:中国标准出版社,2009.
[17] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6435-2014饲料中水分的测定[S].北京:中国标准出版社,2015.
[18] 国家市场监督管理总局国家标准化管理委员会.GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法[S].北京:中国标准出版社,2018.
[19] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员.GB/T 6433-2006饲料中粗脂肪的测定[S].北京:中国标准出版社,2006.
[20] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 20806-2006饲料中中性洗涤纤维(NDF)的测定[S].北京:中国标准出版社,2007.
[21] 中华人民共和国农业部.NY/T 1459-2007饲料中酸性洗涤纤维的测定[S].北京:农业出版社,2008.
[22] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6438-2007饲料中粗灰分的测定[S].北京:中国标准出版社,2007.
[23] 国家市场监督管理总局,中国国家标准化管理委员会.GB/T 6436-2018饲料中钙的测定[S].北京:中国标准出版社,2018.
[24] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6437-2018饲料中总磷的测定分光光度法[S].北京:中国标准出版社,2018.
[25] WALLMANN J,BÖTTNER A,GOOSSENS L,et al.Results of an interlaboratory test on antimicrobial susceptibility testing of bacteria from animals by broth microdilution[J].International Journal of Antimicrobial Agents,2006,27(6):482-490.

[26] BYARUGABA D K.Antimicrobial resistance in developing countries and responsible risk factors[J].International Journal of Antimicrobial Agents,2004,24(2):105-110.

[27] CASEWELL M,FRIIS C,MARCO E,et al.The European ban on growth-promoting antibiotics and emerging consequences for human and animal health[J].Journal of Antimicrobial Chemotherapy,2003,52(2):159-161.

[28] OH J,HARPER M,GIALLONGO F,et al.Effects of rumen-protected Capsicum oleoresin on productivity and responses to a glucose tolerance test in lactating dairy cows[J].Journal of Dairy Science,2017,100(3):1888-1901.

[29] TAGER L R,KRAUSE K M.Effects of essential oils on rumen fermentation,milk production,and feeding behavior in lactating dairy cows[J].Journal of Dairy Science,2011,94(5):2455-2464.

[30] YANG W Z,AMETAJ B N,BENCHAAR C,et al.Cinnamaldehyde in feedlot cattle diets:intake,growth performance,carcass characteristics,and blood metabolites[J].Journal of Animal Science,2010,88(3):1082-1092.

[31] BENCHAAR C.Diet supplementation with cinnamon oil,cinnamaldehyde,or monensin does not reduce enteric methane production of dairy cows[J].Animal,2016,10(3):418-425.

[32] 斯琴毕力格,王丽芳,丁赫,等.饲粮中添加黄花蒿乙醇提取物对奶牛产奶性能及乳脂中共轭亚油酸含量的影响[J].动物营养学报,2017,29(11):4137-4142.
[33] 张成喜,刘开东,孙国强.肉桂醛对奶牛尿中嘌呤衍生物排出量、产奶性能和氮排泄的影响[J].动物营养学报,2017,29(6):2010-2017.
[34] 刘景喜,芦娜,冯晋芳,等.复合植物提取物对奶牛产奶量、乳品质及经济效益的影响[J].中国奶牛,2019(3):1-4.
[35] 莫慧诚,杨燕宁,林鸿.大蒜油和肉桂醛复合物对奶牛物质体外消化率、血清尿素氮以及葡萄糖的影响[J].中国饲料,2018(2):34-37.
[36] WALL E H,DOANE P H,DONKIN S S,et al.The effects of supplementation with a blend of cinnamaldehyde and eugenol on feed intake and milk production of dairy cows[J].Journal of Dairy Science,2014,97(9):5709-5717.

[37] 扈添琴,韩兆玉,王群,等.酶制剂和植物甾醇复合物对泌乳奶牛生产性能和血清指标的影响[J].动物营养学报,2014,26(1):236-244.
[38] 史仁煌,毛江,杜云,等.杜仲素对泌乳高峰期奶牛瘤胃发酵和血清指标的影响[J].中国畜牧杂志,2015,51(7):37-41.
[39] 张成喜,孙国强.过瘤胃蛋氨酸和肉桂醛对奶牛血液生化指标的影响[J].饲料工业,2018,39(1):32-35.
[40] 王建东,马吉锋,侯鹏霞,等.枸杞多糖对围产期奶牛抗体水平和细胞因子分泌的影响[J].动物医学进展,2018,39(4):68-71.
[41] 张吉鹍.植物提取物对奶牛的营养生理功能及其最新研究进展[J].中国奶牛,2018(9):1-6.
[42] 黄其春,郑新添,黄翠琴,等.银杏叶超微粉对断奶仔猪生长性能、肠道菌群及其形态的影响[J].中国畜牧杂志,2018,54(11):105-109.
[43] 朱碧泉,曹璐,车炼强,等.植物提取物对育肥猪生长性能、胴体性状、猪肉品质及抗氧化能力的影响[J].中国饲料,2011(14):15-18.
[44] 阿顺贤,罗增海,张文颖,等.菊苣酸对围产期放牧牦牛生长性能、血清生化指标及抗氧化能力的影响[J].中国畜牧兽医,2019,46(2):449-457.
[45] GROSSMAN C J.Interactions between the gonadal steroids and the immune system[J].Science,1985,227(4684):257-261.

[46] ADJEI A A,MORIOKA T,AMEHO C K,et al.Nucleoside-nucleotide free diet protects rat colonic mucosa from damage induced by trinitrobenzene sulphonic acid[J].Gut,1996,39(3):428-433.

[47] 罗国富,杨小萍,宋焕,等.外源核苷酸对动物营养的作用[J].饲料博览,2009(4):8-11.
[48] 卢德勋.新版系统动物营养学导论[M].北京:中国农业出版社,2016:427-465.

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