本试验旨在研究全株玉米青贮中植物乳杆菌及其与尿素混合添加对杜泊绵羊消化代谢、血清生化指标及瘤胃发酵指标的影响，为全株玉米青贮饲料在育肥羊上更好地利用提供科学依据。试验选取60只体重[（23.0±1.5） kg]相近、健康状况良好的杜泊育肥期绵羊，随机分为3组，每组4个重复，每个重复5只羊。3组羊分别饲喂由不含任何添加剂的全株玉米青贮（对照组）、单一添加占全株玉米鲜重0.1%的植物乳杆菌的全株玉米青贮（益生菌组）和混合添加占全株玉米鲜重0.1%的植物乳杆菌与0.5%的尿素的全株玉米青贮（混合组）配制而成的全混合日粮。预试期10 d，正试期90 d。结果表明：1）混合组的粗蛋白质（CP）、粗灰分（Ash）、钙（Ca）、中性洗涤纤维（NDF）摄入量显著高于益生菌组和对照组（P<0.05）。混合组的食入氮（IN）、可消化氮（DN）、沉积氮（RN）、氮的消化率（NAD）及氮的利用率（NUR）显著高于对照组（P<0.05），且Ash和NDF表观消化率显著高于对照组（P<0.05）。2）益生菌组的血清白蛋白（ALB）、甘油三酯（TG）含量及谷丙转氨酶（ALT）、谷草转氨酶（AST）活性显著高于对照组（P<0.05），而血清γ-谷氨酰转肽酶（γ-GGT）活性显著低于对照组（P<0.05）。混合组的血清TG含量显著高于对照组（P<0.05），血清γ-GGT活性显著低于对照组（P<0.05）。3）混合组的瘤胃液氨氮含量显著高于对照组和益生菌组（P<0.05）；益生菌组的瘤胃液乙酸和总挥发性脂肪酸（TVFA）含量显著高于对照组（P<0.05），pH显著低于对照组（P<0.05）；混合组的瘤胃液丁酸含量显著低于对照组和益生菌组（P<0.05）。由此可见，饲喂在全株玉米青贮中单一添加植物乳杆菌的全混合日粮并不能改变杜泊绵羊消化代谢指标，饲喂在全株玉米青贮中混合添加植物乳杆菌和尿素的全混合日粮有利于氮的消化吸收和利用，同时影响了杜泊绵羊血清生化指标和瘤胃发酵指标。

This experiment was conducted to study the effects of Lactobacillus plantarum and it mixed with urea added in whole corn silage on digestion and metabolism, serum biochemical and rumen fermentation indexes of Dorper sheep, to provide scientific basis for better utilization of whole corn silage fattening sheep. Sixty healthy male Dorper sheep in fattening period with similar body weight[(23.0±1.5) kg] were selected and randomly divided into 3 groups with 4 replicates per group and 5 sheep per replicate. Sheep were fed with no additives whole corn silage (control group), single addition of 0.1% Lactobacillus plantarum of whole corn fresh weight (probiotics group) and 0.1% Lactobacillus plantarum mixed with 0.5% urea mixture of whole corn fresh weight (mixed group) formulated total mixed rations, respectively. The pre-experimental period lasted for 10 days, and the experimental period lasted for 90 days. The results showed as follows:1) the intakes of crude protein (CP), crude ash (Ash), calcium (Ca) and neutral washing fiber (NDF) of mixed group were significantly higher than those of probiotics group and control group (P<0.05). The nitrogen intake (IN), digestible nitrogen (DN), retention nitrogen (RN) and nitrogen digestibility (NAD) and nitrogen utilization ratio (NUR) of mixed group were significantly higher than those of the control group (P<0.05), and the apparent digestibilities of Ash and NDF were significantly higher than those of the control group (P<0.05). 2) The albumin (ALB), triglyceride (TG) contents and alanine aminotransferase (ALT), glutamate aminotransferase (AST) activities in serum of probiotics group were significantly higher than those of the control group (P<0.05), and the serum γ-glutamyltransferase (γ-GGT) activity was significantly lower than that of the control group (P<0.05). The serum TG content of mixed group was significantly higher than that of the control group (P<0.05), and the serum γ-GGT activity was significantly lower than that of the control group (P<0.05). 3) The rumen fluid ammonia nitrogen content of mixed group was significantly higher than that of probiotics group and control group (P<0.05); the contents of acetic acid and total volatile fatty acid (TVFA) in rumen fluid of probiotics group were significantly higher than those of the control group (P<0.05), and the pH was significantly lower than that of the control group (P<0.05); the rumen fluid butyric acid content of mixed group was significantly lower than that of probiotics group and control group (P<0.05). In conclusion, the single addition of Lactobacillus plantarum in whole corn silage total mixed ration cannot change the digestion and metabolism indexes of Dorper sheep, and the addition of Lactobacillus plantarum mixed with urea in whole corn silage total mixed ration is conducive to the digestion and absorption of nitrogen, and affect the serum biochemical indexes and rumen fermentation indexes of Dorper sheep.