饲料科学与技术 Feed science and technology

饲料中添加刺五加超微粉对吉富罗非鱼生长、脂肪沉积以及非特异性免疫能力的影响

  • 李鸣霄 ,
  • 李红霞 ,
  • 强俊 ,
  • 徐跑 ,
  • 包景文 ,
  • 陈德举 ,
  • 陶易凡 ,
  • 朱昊俊
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  • 1. 南京农业大学无锡渔业学院, 无锡 214081;
    2. 中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业与种质资源利用重点实验室, 无锡 214081
李鸣霄(1997-),男,浙江兰溪人,硕士研究生,研究方向为水产动物繁殖生物学。E-mail:535613003@qq.com

收稿日期: 2019-05-23

  网络出版日期: 2019-12-13

基金资助

中国水产科学研究院淡水渔业研究中心基本科研业务费专项项目(2019JBFC01)

Effects of Acanthopanax senticosus Ultrafine Powder on Growth, Fat Deposition and Non-Specific Immunity of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

  • LI Mingxiao ,
  • LI Hongxia ,
  • QIANG Jun ,
  • XU Pao ,
  • BAO Jingwen ,
  • CHEN Deju ,
  • TAO Yifan ,
  • ZHU Haojun
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  • 1. Wuxi Fisheries College, Nanjing Agricultural University, Wu'xi 214081, China;
    2. Key Laboratory of Freshwater Fishes and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wu'xi 214081, China

Received date: 2019-05-23

  Online published: 2019-12-13

摘要

本试验旨在探究饲料中添加刺五加超微粉对吉富罗非鱼生长、脂肪沉积以及非特异性免疫能力的影响。选取平均体重为(6.50±0.02) g的健康吉富罗非鱼720尾,随机分成6组,每组4个重复,每个重复放养30尾鱼。6组试验鱼分别饲喂添加了0(对照)、0.5‰、1.0‰、2.0‰、4.0‰和8.0‰刺五加超微粉的试验饲料,饲养56 d。试验结束后,测定各组试验鱼的生长性能、血常规指标、血清生化指标、肝脏生化指标以及肝脏中免疫相关基因mRNA相对表达量。结果表明:1)各添加组的增重了(WGR)和特定生长率(SGR)均显著高于对照组(P<0.05),且以2.0‰组最高。1.0‰、2.0‰、4.0‰、8.0‰组饲料转化率(FCR)均显著高于对照组(P<0.05),且以1.0‰组最高。2)2.0‰、4.0‰、8.0‰组的白细胞计数(WBC)与8.0‰组红细胞计数(RBC)均显著高于对照组(P<0.05),但在血红蛋白浓度(HGB)和红细胞压积(HCT)上各组之间均没有显著差异(P>0.05)。3)血清葡萄糖(Glu)含量随刺五加添加量的增加呈先下降后上升的趋势,0.5‰和1.0‰组显著低于其他组(P<0.05)。1.0‰组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性以及甘油三酯(TG)含量均最低,显著低于对照组(P<0.05)。血清补体3(C3)含量随饲料中刺五加超微粉添加量的增加先上升后下降,以1.0‰组最高,显著高于对照组(P<0.05)。血清溶菌酶(LZM)活性以1.0‰组最高,与对照组差异不显著(P>0.05),但显著高于8.0‰组(P<0.05)。4)2.0‰组肝脏超氧化物歧化酶(SOD)和LZM活性显著高于对照组(P<0.05),同时该组肝脏丙二醛(MDA)含量最低,显著低于对照组(P<0.05)。肝脏总胆固醇(TC)、糖原(Gly)和TG含量随饲料中刺五加超微粉添加量的增加呈先下降后上升的趋势,1.0‰组显著低于对照组(P<0.05)。5)与对照组相比,各添加组肝脏中肿瘤坏死因子-α(TNF-α)与γ-干扰素(INF-γ) mRNA相对表达量均显著降低(P<0.05)。综上,在实际生产中,建议在饲料中添加1.0‰~2.0‰的刺五加超微粉,以促进吉富罗非鱼健康生长。

本文引用格式

李鸣霄 , 李红霞 , 强俊 , 徐跑 , 包景文 , 陈德举 , 陶易凡 , 朱昊俊 . 饲料中添加刺五加超微粉对吉富罗非鱼生长、脂肪沉积以及非特异性免疫能力的影响[J]. 动物营养学报, 2019 , 31(12) : 5801 -5812 . DOI: 10.3969/j.issn.1006-267x.2019.12.046

Abstract

The aim of this study was to investigate the effects of Acanthopanax senticosus (AS) ultrafine powder on growth, fat deposition and non-specific immunity of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). A total of 720 healthy GIFT with an average body weight of (6.50±0.02) g were randomly divided into 6 groups with 4 replicates per group and 30 fish per replicate. Six experimental diets which supplemented with 0 (control), 0.5‰, 1.0‰, 2.0‰, 4.0‰ and 8.0‰ AS ultrafine powder were used to feed fish in the 6 groups. After feeding 56 days, the growth performance, blood routine indexes, serum biochemical indexes, liver biochemical indexes and the mRNA relative expression levels of immune related genes in liver of fish in each group were determined. The results showed as follows:1) the weight gain rate and specific growth rate of each additive group were significantly higher than those of control group (P<0.05), and the highest values were all found in the 2.0‰ group. The feed conversion ratio of 1.0‰, 2.0‰, 4.0‰ and 8.0‰ groups was significantly higher than that of control group (P<0.05), and the highest value was found in the 1.0‰ group. 2) The white blood cell count of 2.0‰, 4.0‰, 8.0‰ groups and the red blood cell count of 8.0‰ group were significantly higher than those of control group (P<0.05), but there were no significant differences among groups in hemoglobin concentration and hematocrit (P>0.05). 3) The serum glucose content decreased first and then increased with the increase of AS ultrafine powder supplementation, and that of 0.5‰ and 1.0‰ groups was significantly lower than that of other groups (P<0.05). The serum alanine aminotransferase, aspartate aminotransferase activities and triglyceride content were the lowest in the 1.0‰ group, which were significantly lower than those of control group (P<0.05). The content of serum complement 3 increased first and then decreased with the increase of AS ultrafine powder supplementation, and the highest value was found in the 1.0‰ group, which was significantly higher than that of control group (P<0.05). The activity of serum lysozyme was the highest in the 1.0‰ group, which was not significantly different from the control group (P>0.05), but significantly higher than the 8.0‰ group (P<0.05). 4) The activities of superoxide dismutase and lysozyme in liver of 2.0‰ group were significantly higher than those of control group (P<0.05). The content of malondialdehyde in liver was the lowest in the 2.0‰ group, which was significantly lower than that of control group (P<0.05). The contents of total cholesterol, glycogen and triglyceride in liver decreased first and then increased with the increase of AS ultrafine powder supplementation, and the 1.0‰ group was significantly lower than the control group (P<0.05). 5) Compared with the control group, the mRNA relative expression levels of tumor necrosis factor-α and interferon-γ in liver of each additive group were significantly decreased (P<0.05). Brief, in actual farming, it is recommended to add 1.0‰ to 2.0‰ of AS ultrafine powder to the diet, which can promote the healthy growth of GIFT.

参考文献

[1] 齐遵利,张秀文,孙步太,等.复方中草药饲料添加剂对草鱼生长性能和免疫保护力的影响[J].科学养鱼,2009(12):64-66.
[2] 许友卿,丁兆坤.水产动物饲料添加剂促进营养与免疫的研究[J].水产科学,2013,32(5):300-305.
[3] 孔江红.复方中草药对斜带石斑鱼生长性能及非特异性免疫功能的影响[D].硕士学位论文.福州:集美大学,2010.
[4] 段雪磊,马奎红,包永占,等.刺五加免疫调节功能的研究进展[J].中兽医学杂志,2015,(6):72-75.
[5] YI J M,KIM M S,SEO S W,et al.Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis[J].Clinica Chimica Acta,2001,312(1):163-168.
[6] GAFFNEY B T,HÜGEL H M,RICH P A.Panax ginseng and Eleutherococcus senticosus may exaggerate an already existing biphasic response to stress via inhibition of enzymes which limit the binding of stress hormones to their receptors[J].Medical Hypotheses,2001,56(5):567-572.  
[7] GUO M Q,SONG F R,LIU Z Q,et al.Characterization of triterpenoidic saponin mixture in crude extracts from leaves of Acanthopanax senticosus Harms by saponin structural correlation and mass spectrometry[J].Analytica Chimica Acta,2006,557(1/2):198-203.
[8] CHEN R Z,LIU Z Q,ZHAO J M,et al.Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu[J].Food Chemistry,2011,127(2):434-440.  
[9] FANG J,YAN F Y,KONG X F,et al.Dietary supplementation with Acanthopanax senticosus extract enhances gut health in weanling piglets[J].Livestock Science,2009,123(2/3):268-275.
[10] YAMAZAKI T,SHIMOSAKA S,SASAKI H,et al.(+)-syringaresinol-di-O-β-D-glucoside,a phenolic compound from Acanthopanax senticosus Harms,suppresses proinflammatory mediators in SW982 human synovial sarcoma cells by inhibiting activating protein-1 and/or nuclear factor-κB activities[J].Toxicology in Vitro,2007,21(8):1530-1537.  
[11] 陈思,孙春玉,王义.药用植物刺五加研究进展[J].园艺与种苗,2011(4):108-111.
[12] 陈芬芳.刺五加多糖对肉鸡免疫功能和抗氧化性能的研究[D].硕士学位论文.聊城:聊城大学,2015.
[13] YOON T J,YOO Y C,LEE S W,et al.Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action[J].Journal of Ethnopharmacology,2004,93(2/3):247-253.
[14] KONG X F,YIN Y L,WU G Y,et al.Dietary supplementation with Acanthopanax senticosus extract modulates cellular and humoral immunity in weaned piglets[J].Asian-Australasian Journal of Animal Sciences,2007,20(9):1453-1461.  
[15] 阮国良,杨代勤,王金龙.几种中草药饲料添加剂对黄鳝免疫功能和生长性能的影响[J].饲料工业,2005,26(24):34-36.
[16] 王永玲,蔡春芳.中草药免疫增强剂对银鲫促生长效果的研究[J].水生态学杂志,2002,22(4):42-43.
[17] 陈开文,谭涌.中药超微粉碎应用研究概况[J].中国药业,2006,15(2):75-77.
[18] QIANG J,YANG H,WANG H,et al.Interacting effects of water temperature and dietary protein level on hematological parameters in Nile tilapia juveniles,Oreochromis niloticus (L.) and mortality under Streptococcus iniae infection[J].Fish & Shellfish Immunology,2013,34(1):8-16.  
[19] MA X Y,QIANG J,HE J,et al.Changes in the physiological parameters,fatty acid metabolism,and SCD activity and expression in juvenile GIFT tilapia (Oreochromis niloticus) reared at three different temperatures[J].Fish Physiology and Biochemistry,2015,41(4):937-950.  
[20] TAO Y F,QIANG J,BAO J W,et al.miR-205-5p negatively regulates hepatic acetyl-CoA carboxylase β mRNA in lipid metabolism of Oreochromis niloticus[J].Gene,2018,660:1-7.
[21] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.  
[22] 谢丽玲,曹俊辉,杨素霞,等.中草药作为饲料添加剂养殖罗非鱼试验[J].水产科学,2009,28(1):11-14.
[23] 于明超,李卓佳,林黑着,等.饲料中添加芽孢杆菌和中草药制剂对凡纳滨对虾生长及肠道菌群的影响[J].热带海洋学报,2010,29(4):132-137.
[24] 黄凯,杨鸿昆,甘晖,等.饲料中添加胆碱预防罗非鱼脂肪肝病变的作用[J].中国水产科学,2007,14(2):257-262.
[25] 张海涛,王安利,李国立,等.营养素对鱼类脂肪肝病变的影响[J].海洋通报,2004,23(1):82-89.
[26] BHASKAR B R,RAO K S.Influence of environmental variables on haematology,and compendium of normal haematological ranges of milkfish,Chanos chanos (Forskal),in brackishwater culture[J].Aquaculture,1989,83(1/2):123-136.
[27] HARIKRISHNAN R,KIM M C,KIM J S,et al.Protective effect of herbal and probiotics enriched diet on haematological and immunity status of Oplegnathus fasciatus (Temminck & Schlegel) against Edwardsiella tarda[J].Fish & Shellfish Immunology,2011,30(3):886-893.  
[28] 王军,鄢庆枇,苏永全,等.免疫添加物对大黄鱼血液白细胞数量及其吞噬功能的影响[J].海洋科学,2001,25(9):44-46.
[29] 王晓光.浅析刺五加的药理作用及其临床应用[J].中国医药指南,2014,12(30):271-272.
[30] 刘小玲.应激对黄颡鱼非特异性免疫细胞的影响[D].博士学位论文.武汉:华中农业大学,2006.
[31] QIANG J,YANG H,WANG H,et al.Physiological responses and HSP70 mRNA expression in GIFT tilapia juveniles,Oreochromis niloticus under short-term crowding[J].Aquaculture Research,2015,46(2):335-345.  
[32] CASILLAS E,MYERS M,AMES W E.Relationship of serum chemistry values to liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride[J].Aquatic Toxicology,1983,3(1):61-78.  
[33] 强俊,杨弘,王辉,等.急性温度应激对吉富品系尼罗罗非鱼(Oreochromis niloticus)幼鱼生化指标和肝脏HSP70 mRNA表达的影响[J].海洋与湖沼,2012,43(5):943-953.
[34] 强俊,杨弘,王辉,等.海豚链球菌感染对不同品系罗非鱼血液生化指标和肝脏HSP70 mRNA表达的影响[J].水产学报,2012,36(6):958-968.
[35] LEEMEN C L,LAPPE R,CRESTANI M,et al.Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen[J].Aquaculture,2004,239(1/4):497-507.
[36] LUEDER C G K,ALGNER M,LANG C,et al.Reduced expression of the inducible nitric oxide synthase after infection with Toxoplasma gondii facilitates parasite replication in activated murine macrophages[J].International Journal for Parasitology,2003,33(8):833-844.  
[37] 方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002:1-24.
[38] FRANCO R,SANCHEZ-OLEA R,REYES-REYES E M,et al.Environmental toxicity,oxidative stress and apoptosis:menage a trois[J].Mutation Research/Genetic Toxicology and Environmental Mutagenesis,2009,674(1/2):3-22.
[39] PARIHAR M S,JAVERI T,HEMNANI T,et al.Responses of superoxide dismutase,glutathione peroxidase and reduced glutathione antioxidant defenses in gills of the freshwater catfish (Heteropneustes fossilis) to short-term elevated temperature[J].Journal of Thermal Biology,1997,22(2):151-156.  
[40] 叶继丹,韩友文,赵吉伟,等.喹乙醇对鲤肝胰脏抗氧化酶系统的影响[J].水产学报,2004,28(3):231-235.
[41] HIKIMA S,HIKIMA J I,ROJTINNAKORN J,et al.Characterization and function of kuruma shrimp lysozyme possessing lytic activity against Vibrio species[J].Gene,2003,316:187-195.
[42] HOFFMANN J A,KAFATOS F C,JANEWAY C A,Jr.,et al.Phylogenetic perspectives in innate immunity[J].Science,1999,284(5418):1313-1318.  
[43] GOPALAKANNAN A,ARUL V.Immunomodulatory effects of dietary intake of chitin,chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds[J].Aquaculture,2006,255(1/2/3/4):179-187.
[44] ELLIS A E.Immunity to bacteria in fish[J].Fish & Shellfish Immunology,1999,9(4):291-308.  
[45] ŠKUGOR S,ŠKUGOR A,TODORCEVIC M,et al.Exposure to lipopolysaccharide induces immune genes in cultured preadipocytes of Atlantic salmon[J].Fish & Shellfish Immunology,2010,29(5):817-824.  
[46] NAKAE S,ASANO M,HORAI R,et al.Interleukin-1 beta,but not interleukin-1 alpha,is required for T-cell-dependent antibody production[J].Immunology,2010,104(4):402-409.
[47] VELÁZQUEZ J,ACOSTA J,HERRERA N,et al.Novel IFNγ homologue identified in Nile tilapia (Oreochromis niloticus) links with immune response in gills under different stimuli[J].Fish & Shellfish Immunology,2017,71:275-285.
[48] 王家敏.复方中草药对吉富罗非鱼免疫相关基因表达的影响[D].硕士学位论文.湛江:广东海洋大学,2011.
[49] YI J M,HONG S H,KIM J H,et al.Effect of Acanthopanax senticosus stem on mast cell-dependent anaphylaxis[J].Journal of Ethnopharmacology,2002,79(3):347-352.  
[50] SMITH L C,CLOW L A,TERWILLIGER D P.The ancestral complement system in sea urchins[J].Immunological Reviews,2001,180(1):16-34.  
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