动物营养学报 >

2019 , Vol. 31 >Issue 12: 5834 - 5845

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.12.049

研究简报 Short communications

饲粮添加山竹醇对氧化应激仔猪生长性能、抗氧化功能及肝脏脂质合成的影响

  • 贺琼玉 ,
  • 邵亚飞 ,
  • 姚卫磊 ,
  • 王同心 ,
  • 夏军 ,
  • 黄飞若
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  • 华中农业大学动物科技学院, 武汉 430070
贺琼玉(1995-),女,山东潍坊人,硕士研究生,动物营养与饲料科学专业。E-mail:914352956@qq.com

收稿日期: 2019-05-15

  网络出版日期: 2019-12-13

基金资助

国家重点研发计划（2018YFD0500600）

收起

Effects of Garcinol Supplementation on Growth Performance, Antioxidant Function and Liver Lipid Synthesis of Piglets under Oxidative Stress

  • HE Qiongyu ,
  • SHAO Yafei ,
  • YAO Weilei ,
  • WANG Tongxin ,
  • XIA Jun ,
  • HUANG Feiruo
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  • College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2019-05-15

  Online published: 2019-12-13

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摘要

本试验旨在研究饲粮添加山竹醇对氧化应激仔猪生长性能、抗氧化功能及肝脏脂质合成的影响。选择30头健康状况良好、胎次相近的35日龄三元杂交（杜×长×大）断奶仔猪，随机分为5个组，每组6个重复，每个重复1头仔猪。对照组和应激组仔猪饲喂基础饲粮，山竹醇组仔猪饲喂在基础饲粮中分别添加200、400、600 mg/kg山竹醇的试验饲粮，预饲7 d后开始正式试验，试验期28 d。在试验第15天早晨进行前腔静脉采血，采血后应激组和山竹醇组仔猪按照10 mg/kg BW的剂量腹腔注射敌草快（diquat）溶液，对照组仔猪腹腔注射相同剂量灭菌生理盐水。在试验第29天试验猪空腹进行前腔静脉采血并采取所需肝脏样品，检测血清和肝脏生化指标以及肝脏脂质合成相关基因mRNA的表达量。结果表明：1）注射diquat后（第15~28天），应激组仔猪的平均日增重（ADG）和平均日采食量（ADFI）显著低于对照组（P<0.05），料重比（F/G）显著高于对照组（P<0.05），各山竹醇组的ADG和ADFI均显著高于应激组（P<0.05），400 mg/kg山竹醇组的F/G显著低于应激组（P<0.05）。2）注射diquat前（第15天），饲粮添加400、600 mg/kg山竹醇显著提高了仔猪血清超氧化物歧化酶（SOD）活性以及总抗氧化能力（T-AOC）（P<0.05）。注射diquat后（第29天），与对照组相比，应激组血清和肝脏SOD、谷胱甘肽过氧化物酶（GSH-Px）活性显著降低（P<0.05），血清和肝脏丙二醛（MDA）含量显著增加（P<0.05）；饲粮添加200、400和600 mg/kg山竹醇均显著提高了氧化应激仔猪血清和肝脏SOD、GSH-Px活性（P<0.05），显著降低了血清和肝脏MDA含量（P<0.05），其中以山竹醇添加量为400 mg/kg时效果最好。3）肝脏组织病理学观察发现应激组仔猪肝脏组织结构受到损伤，且肝脏内脂质沉积明显增加，与应激组相比，各山竹醇组的肝脏组织结构有一定程度恢复，除此之外，脂质沉积也明显减少。4）注射diquat后，应激组血清甘油三酯（TG）和总胆固醇（TC）含量显著高于对照组（P<0.05）。饲粮添加400、600 mg/kg山竹醇可显著降低氧化应激仔猪血清TG和TC含量（P<0.05），且以山竹醇添加量为400 mg/kg时血清TG和TC含量最低。5）与对照组相比，应激组肝脏固醇调节元件结合蛋白-1c （SREBP-1c）、脂肪酸合成酶（FAS）、乙酰辅酶A羧化酶（ACC）、硬脂酰辅酶A去饱和酶1（SCD1） mRNA的表达量显著上调（P<0.05）。饲粮添加200、600 mg/kg山竹醇可以显著降低氧化应激仔猪肝脏中SREBP-1c、ACC mRNA的表达量（P<0.05）；饲粮添加400 mg/kg山竹醇可以显著降低氧化应激仔猪肝脏中SREBP-1c、FAS、ACC、SCD1 mRNA的表达量（P<0.05）。由此可见，氧化应激条件下，饲粮添加山竹醇可通过改善仔猪的抗氧化能力，缓解diquat诱导的氧化应激，改善应激仔猪的生长性能以及应激导致的脂质合成增多，保护肝脏，其中以山竹醇添加量为400 mg/kg时效果最佳。

关键词： 山竹醇; 仔猪; 抗氧化; 肝脏; 脂质合成

本文引用格式

贺琼玉 , 邵亚飞 , 姚卫磊 , 王同心 , 夏军 , 黄飞若 . 饲粮添加山竹醇对氧化应激仔猪生长性能、抗氧化功能及肝脏脂质合成的影响[J]. 动物营养学报, 2019 , 31(12) : 5834 -5845 . DOI: 10.3969/j.issn.1006-267x.2019.12.049

Abstract

This study was conducted to evaluate the effects of garcinol supplementation on growth performance, antioxidant function and liver lipid synthesis of piglets under oxidative stress. Thirty 35-day-old three-breed crossbred (Duroc×Landrace×Yorkshire) piglets with healthy condition and similar parity were randomly divided into 5 groups with 6 replicates in each group and 1 piglet in each replicate. The piglets in control group and stress group were fed a basal diet, and the others in garcinol groups were fed the basal diet supplemented with 200, 400 and 600 mg/kg garcinol, respectively. After 7 days of pre-feeding, the formal trial was started. The experiment lasted for 28 days. Blood samples were sampled via anterior vena cava in the morning of the 15th day. Then the piglets in stress group and garcinol groups were injected intraperitoneally with diquat solution at the dose of 10 mg/kg BW and piglets in control group were injected with the same volume of sterilized saline. In the morning of the 29th day, blood samples were fasting collected via anterior vena cava and the liver samples were collected after slaughter for detection of biochemical indexes, as well as the mRNA expression levels of liver lipid synthesis related genes. The results shows as follows:1) after diquat injection (the 15th to 28th day), average daily gain (ADG) and average daily feed intake (ADFI) of piglets in stress group were significantly decreased and feed/gain (F/G) was significantly increased compared with control group (P<0.05). The ADG and ADFI of piglets in three garcinol groups were significantly higher than those in stress group (P<0.05), while the F/G in 400 mg/kg garcinol group was significantly lower than that in stress group (P<0.05). 2) Before diquat injection (the 15th day), diet supplemented 400 and 600 mg/kg garcinol significantly increased the activity of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum (P<0.05). After diquat injection (the 29th day), the activity of SOD and glutathione peroxidase (GSH-Px) in serum and liver in stress group were significantly decreased (P<0.05), and the malondialdehyde (MDA) content was significantly increased compared with control group (P<0.05). Diet supplemented 200, 400 and 600 mg/kg garcinol significantly increased the activities of SOD and GSH-Px and significantly decreased the content of MDA in serum and liver of piglets under oxidative stress (P<0.05), among which the effect of garcinol at 400 mg/kg was the best. 3) Histological observation showed that the liver tissue structure of the stress group was damaged, and the lipid deposition in the liver was obviously increased. The tissue structure of liver in garcinol groups recovered to a certain extent, and the lipid deposition was also obviously reduced compared with the stress group. 4) After diquat injection (the 29th day), the contents of triglycerides (TG) and total cholesterol (TC) in serum in stress group were significantly higher than those in control group (P<0.05). Diet supplemented 200, 400 and 600 mg/kg garcinol significantly decreased the contents of TG and TC in serum of piglets under oxidative stress (P<0.05), and they were the lowest when the supplemental level of garcinol was 400 mg/kg. 5) Compared with the control group, the mRNA expression levels of sterol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and stearoyl-CoA desaturase 1 (SCD1) in liver the stress group were significantly up-regulated (P<0.05). When diet supplemented with 200 and 600 mg/kg garcinol, the mRNA expression levels of SREBP-1c and ACC in liver of piglets under oxidative stress were significantly down-regulated (P<0.05). When diet supplemented with 400 mg/kg garcinol, the mRNA expression levels of SREBP-1c, FAS, ACC and SCD1 in liver of piglets under oxidative stress were significantly down-regulated (P<0.05). The results indicate that garcinol supplementation can alleviate oxidative stress induced by diquat, improve the growth performance and lipid synthesis of piglets under oxidative stress states by improving antioxidant capacity. The best effect is achieved when the supplemental level of garcinol is 400 mg/kg.

Key words： garcinol; piglets; antioxidant; liver; lipid synthesis

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