动物营养学报 >

2020 , Vol. 32 >Issue 1: 99 - 108

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.015

猪营养与饲料 SWINE NUTRITION AND FEED

柑橘提取物对仔猪免疫功能的影响

  • 崔艺燕 ,
  • 田志梅 ,
  • 邓盾 ,
  • 王刚 ,
  • 鲁慧杰 ,
  • 陈卫东 ,
  • 马现永
展开
  • 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640
崔艺燕(1987-),女,广东佛山人,助理研究员,硕士,从事动物营养与饲料科学、生态养殖与环境控制的研究。E-mail:958117076@qq.com

收稿日期: 2019-07-18

  网络出版日期: 2020-01-19

基金资助

广东省现代农业产业技术体系创新团队项目（2018LM1080）；省级现代农业产业技术推广体系建设项目（2018LM2153）；动物健康养殖国际科技合作示范基地（2019A050505007）；清远市启航计划（2018002）；广东省农业科学院人才项目（[2018]3）

收起

Effects of Citrus Extract on Immunity of Piglets

  • CUI Yiyan ,
  • TIAN Zhimei ,
  • DENG Dun ,
  • WANG Gang ,
  • LU Huijie ,
  • CHEN Weidong ,
  • MA Xianyong
Expand
  • Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Key Laboratory of Animal Breeding and Nutrition, The Key Laboratory of Animal Nutrition and Feed Science(South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2019-07-18

  Online published: 2020-01-19

Supported by

 

Fold

摘要

本试验旨在研究柑橘提取物对仔猪血清、肝脏以及十二指肠、空肠黏膜免疫功能的影响。选取144头28日龄（8.39±1.06）kg的"杜×长×大"健康仔猪，按体重随机分配到3个组，每个组6个栏（重复），每个栏8头猪，公母各占1/2。3个组分别为：对照组，饲喂基础饲粮；抗生素组，在基础饲粮上添加75 g/t金霉素；柑橘提取物组，在基础饲粮上添加300 mL/t柑橘提取物。试验期28 d。结果表明：1）与对照组相比，抗生素组和柑橘提取物组仔猪血清免疫球蛋白G（IgG）含量分别显著提高了11.27%和14.01%（P<0.05）；与抗生素组和对照组相比，柑橘提取物组仔猪血清白细胞介素-6（IL-6）和免疫球蛋白A（IgA）含量有提高趋势（0.05 ≤ P < 0.10）；柑橘提取物组仔猪血清肿瘤坏死因子-α（TNF-α）含量较对照组显著提高了12.13%（P<0.05），与抗生素组差异不显著（P>0.05）。2）与对照组相比，柑橘提取物组和抗生素组仔猪肝脏免疫球蛋白M和IgG含量显著下降（P<0.05）；与对照组和柑橘提取物组相比，抗生素组仔猪肝脏TNF-α含量显著降低（P<0.05），肝脏IgA含量有降低趋势（0.05 ≤ P < 0.10）。3）3组间仔猪十二指肠黏膜免疫指标差异不显著（P>0.05）。4）与对照组相比，柑橘提取物和抗生素有提高仔猪空肠黏膜白细胞介素-2和IL-6含量的趋势（0.05 ≤ P < 0.10），抗生素组仔猪空肠黏膜白细胞介素-8含量显著提高（P<0.05）；柑橘提取物组仔猪空肠黏膜分泌型免疫球蛋白A含量最高，较对照组提高了19.35%（0.05 ≤ P < 0.10）。由此可见，柑橘提取物和抗生素提高了仔猪血清和空肠黏膜的细胞因子和免疫球蛋白含量，增强了仔猪免疫功能。

关键词： 柑橘提取物; 仔猪; 免疫

本文引用格式

崔艺燕 , 田志梅 , 邓盾 , 王刚 , 鲁慧杰 , 陈卫东 , 马现永 . 柑橘提取物对仔猪免疫功能的影响[J]. 动物营养学报, 2020 , 32(1) : 99 -108 . DOI: 10.3969/j.issn.1006-267x.2020.01.015

Abstract

This experiment was conducted to investigate the effects of citrus extract on immune function in serum, liver, duodenal and jejunal mucosa of piglets. One hundred and forty-four 28-day-old (8.39±1.06) kg Duroc×Landrace×Large White pigs were randomly assigned to 3 groups with 6 pens (replicates) per group and 8 pigs per pen (half male and half female). Three groups were:control group, fed a basal diet; antibiotic group, fed the basal diet supplemented with 75 g/t chlortetracycline; citrus extract group, fed the basal diet supplemented with 300 mL/t citrus extract. The experiment lasted for 28 days. The results showed as follows. 1) compared with the control group, the serum immunoglobulin G (IgG) content of piglets in the antibiotic group and the citrus extract group was significantly increased by 11.27% and 14.01%, respectively (P<0.05); compared with the antibiotic group and the control group, the contents of serum interleukin-6 (IL-6) and immunoglobulin G (IgA) of piglets in the citrus extract group were tended to be increased (0.05 ≤ P < 0.10); the serum tumor necrosis factor-α (TNF-α) content of piglets in the citrus extract group was significantly increased by 12.13% compared with the control group (P<0.05), but not significantly different from that in the antibiotic group (P>0.05). 2) Compared with the control group, the contents of liver immunoglobulin M and IgG of piglets in the citrus extract group and the antibiotic group were significantly decreased (P<0.05). Compared with the control group and the citrus extract group, the liver TNF-α content of piglets in the antibiotic group was significantly decreased (P<0.05), and liver IgA content had a decreasing trend (0.05 ≤ P < 0.10). 3) There was no significant difference in indices of duodenal mucosal immunity among three groups (P>0.05). 4) Compared with the control group, the citrus extract and antibiotic had a trend to increase contents of interleukin-2 and IL-6 in jejunal mucosa of piglets (0.05 ≤ P < 0.10), and the IL-8 content in jejunal mucosa of piglets in the antibiotic group was significantly increased (P<0.05). The secretory immunoglobulin A content in jejunal mucosa of piglets in the citrus extract group was the highest, which was 19.35% higher than that in the control group (0.05 ≤ P < 0.10). In brief, citrus extract and antibiotic can improve the contents of cytokines and immunoglobulins in serum and jejunal mucosa, thus enhance the immunity of piglets.

Key words： citrus extract; piglets; immunity

参考文献

[1] PLUSKE J R,HAMPSON D J,WILLIAMS I H.Factors influencing the structure and function of the small intestine in the weaned pig:a review[J].Livestock Production Science,1997,51(1/2/3):215-236.
[2] CAMPBELL J M,CRENSHAW J D,POLO J.The biological stress of early weaned piglets[J].Journal of Animal Science and Biotechnology,2013,4(1):19.
[3] SWEENEY T,O'DOHERTY J V.Marine macroalgal extracts to maintain gut homeostasis in the weaning piglet[J].Domestic Animal Endocrinology,2016,56(Suppl.):S84-S89.
[4] KIM A,IM M,GU M J,et al.Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma[J].Scientific Reports,2016,6(1):24214.
[5] GARCIA A R,AMARAL A C F,AZEVEDO M M B,et al.Cytotoxicity and anti-Leishmania amazonensis activity of Citrus sinensis leaf extracts[J].Pharmaceutical Biology,2016,55(1):1780-1786.
[6] TOSCANO-GARIBAY J D,ARRIAGA-ALBA M,SÁNCHEZ-NAVARRETE J,et al.Antimutagenic and antioxidant activity of the essential oils of Citrus sinensis and Citrus latifolia[J].Scientific Reports,2017,7(1):11479.
[7] NDAYISHIMIYE J,LIM D J,CHUN B S.Antioxidant and antimicrobial activity of oils obtained from a mixture of citrus by-products using a modified supercritical carbon dioxide[J].Journal of Industrial and Engineering Chemistry,2018,57:339-348.
[8] WANG J P,QI Y,NIU X L,et al.Dietary naringenin supplementation attenuates experimental autoimmune encephalomyelitis by modulating autoimmune inflammatory responses in mice[J].The Journal of Nutritional Biochemistry,2018,54:130-139.
[9] GOODARZI BOROOJENI F,MÄNNER K,ZENTEK J.The impacts of Macleaya cordata extract and naringin inclusion in post-weaning piglet diets on performance,nutrient digestibility and intestinal histomorphology[J].Archives of Animal Nutrition,2018,72(3):178-189.  
[10] ALHIDARY I A,ABDELRAHMAN M M.Effects of naringin supplementation on productive performance,antioxidant status and immune response in heat-stressed lambs[J].Small Ruminant Research,2016,138:31-36.
[11] KAMBOH A A,ZHU W Y.Individual and combined effects of genistein and hesperidin on immunity and intestinal morphometry in lipopolysacharide-challenged broiler chickens[J].Poultry Science,2014,93(9):2175-2183.  
[12] DIAZ-SANCHEZ S,D'SOUZA D,BISWAS D,et al.Botanical alternatives to antibiotics for use in organic poultry production[J].Poultry Science,2015,94(6):1419-1430.  
[13] WINDISCH W,SCHEDLE K,PLITZNER C,et al.Use of phytogenic products as feed additives for swine and poultry[J].Journal of Animal Science,2008,86(14 Suppl.):E140-E148.
[14] VANROLLEGHEM W,TANGHE S,VERSTRINGE S,et al.Potential dietary feed additives with antibacterial effects and their impact on performance of weaned piglets:a meta-analysis[J].The Veterinary Journal,2019,249:24-32.
[15] MIDDLETON E,Jr,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:implications for inflammation,heart disease,and cancer[J].Pharmacological Reviews,2000,52(4):673-751.
[16] LEE Y R,JUNG J H,KIM H S.Hesperidin partially restores impaired immune and nutritional function in irradiated mice[J].Journal of Medicinal Food,2011,14(5):475-482.  
[17] GOLIOMYTIS M,SIMITZIS P,PAPALEXI A,et al.Influence of citrus flavonoids on laying hen performance,inflammatory immune response,egg quality and yolk oxidative stability[J].British Poultry Science,2019,60(3):272-278.  
[18] LI G,CHEN M J,WANG C,et al.Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice[J].International Immunopharmacology,2014,21(2):406-411.  
[19] JUSTIZ VAILLANT A A,QURIE A.Interleukin[M/OL].(2019-06-12)[2019-07-09].https://www.ncbi.nlm.nih.gov/books/NBK499840/.
[20] SZALECKI M,MALINOWSKA A,PROKOP-PIOTRKOWSKA M,et al.Interactions between the growth hormone and cytokines-a review[J].Advances in Medical Sciences,2018,63(2):285-289.  
[21] HAFEZ M M,HAMED S S,EL-KHADRAGY M F,et al.Effect of ginseng extract on the TGF-β1 signaling pathway in CCl4-induced liver fibrosis in rats[J].BMC Complementary and Alternative Medicine,2017,17(1):45.
[22] ESTRUEL-AMADES S,MASSOT-CLADERA M,PÉREZ-CANO F J,et al.Hesperidin effects on gut microbiota and gut-associated lymphoid tissue in healthy rats[J].Nutrients,2019,11(2):324.
[23] HE W,LI Y M,LIU M Y,et al.Citrus aurantium L. and its flavonoids regulate TNBS-induced inflammatory bowel disease through anti-inflammation and suppressing isolated jejunum contraction[J].International Journal of Molecular Sciences,2018,19(10):3057.
[24] STEVENS Y,VAN RYMENANT E,GROOTAERT C,et al.The intestinal fate of citrus flavanones and their effects on gastrointestinal health[J].Nutrients,2019,11(7):1464.
[25] ZANOTTI SIMÕES DOURADO G K,DE ABREU RIBEIRO L,ZEPPONE CARLOS I,et al.Orange juice and hesperidin promote differential innate immune response in macrophages ex vivo[J].International Journal for Vitamin and Nutrition Research,2013,83(3):162-167.  
[26] RAJA KUMAR S,MOHD RAMLI E S,ABDUL NASIR N A,et al.Preventive effect of naringin on metabolic syndrome and its mechanism of action:a systematic review[J].Evidence-Based Complementary and Alternative Medicine,2019,2019:9752826.
[27] PANTSULAIA I,IOBADZE M,PANTSULAIA N,et al.The effect of citrus peel extracts on cytokines levels and T regulatory cells in acute liver injury[J].BioMed Research International,2014,2014:127879.
[28] ISHIDA M,TAKEKUNI C,NISHI K,et al.Anti-inflammatory effect of aqueous extract from Kawachi-bankan (Citrus maxima) peel in vitro and in vivo[J].Cytotechnology,2019,71(4):797-807.  
[29] BISSONNETTE N,JIANG X R,MATTE J J,et al.Effect of a post-weaning diet supplemented with functional feed additives on ileal transcriptome activity and serum cytokines in piglets challenged with lipopolysaccharide[J].Veterinary Immunology and Immunopathology,2016,182:136-149.
[30] MA J L,LI C.A hesperetin derivative plays a role in immunoregulatory effect on human macrophages[J].Cellular and Molecular Biology,2019,65(4):43-47.  
[31] 陈丽.黄褐毛忍冬提取物对早期断奶仔猪生长性能、免疫功能和肠道菌群的影响[D].硕士学位论文.贵阳:贵州师范大学,2017:22-24.
[32] LI X L,HE L P,YANG Y,et al.Effects of extracellular polysaccharides of Ganoderma lucidum supplementation on the growth performance,blood profile,and meat quality in finisher pigs[J].Livestock Science,2015,178:187-194.
[33] XI N,KANG J,HAO L J,et al.Effects of ultrafine powder of the stem and leaf of Astragalus on immunity in chickens[J].Italian Journal of Animal Science,2014,13(1):3022.
[34] WANG D F,ZHOU L L,ZHOU H L,et al.Effects of Piper sarmentosum extract on the growth performance,antioxidant capability and immune response in weaned piglets[J].Journal of Animal Physiology and Animal Nutrition,2017,101(1):105-112.  
[35] LIU Y L,HUANG J J,HOU Y Q,et al.Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs[J].The British Journal of Nutrition,2008,100(3):552-560.  
[36] LI Z L,GAO M,YANG B C,et al.Naringin attenuates MLC phosphorylation and NF-κB activation to protect sepsis-induced intestinal injury via RhoA/ROCK pathway[J].Biomedicine & Pharmacotherapy,2018,103:50-58.
[37] ACIPAYAM C,BAYRAM I,DAGLIOGLU K,et al.The protective effect of hesperidin on methotrexate-induced intestinal epithelial damage in rats:an experimental study[J].Medical Principles and Practice,2014,23(1):45-52.  
[38] GUO K,REN J N,GU G S,et al.Hesperidin protects against intestinal inflammation by restoring intestinal barrier function and up-regulating treg cells[J].Molecular Nutrition & Food Research,2019,63(11):1800975.
[39] HUNG W L,CHANG W S,LU W C,et al.Pharmacokinetics,bioavailability,tissue distribution and excretion of tangeretin in rat[J].Journal of Food and Drug Analysis,2018,26(2):849-857.  
[40] BORMOTOVA E A,GUPALOVA T V.Expression of new recombinant IgG-binding polypeptides and analysis of their capacity to bind human IgG[J].Bulletin of Experimental Biology and Medicine,2018,165(3):373-377.  
[41] CASTRO-DOPICO T,CLATWORTHY M R.IgG and Fcγ receptors in intestinal immunity and inflammation[J].Frontiers in Immunology,2019,10:805.
[42] HANSEN I S,BAETEN D L P,DEN DUNNEN J.The inflammatory function of human IgA[J].Cellular and Molecular Life Sciences,2019,76(6):1041-1055.  
[43] LANGEREIS J D,VAN DER FLIER M,DE JONGE M I.Limited innovations after more than 65 years of immunoglobulin replacement therapy:potential of IgA-and IgM-enriched formulations to prevent bacterial respiratory tract infections[J].Frontiers in Immunology,2018,9:1925.
[44] JUSTIZ VAILLANT A A,RAMPHUL K.Immunoglobulin[M/OL].(2019-06-18)[2019-07-09].https://www.ncbi.nlm.nih.gov/books/NBK513460/.
[45] CAMPS-BOSSACOMA M,FRANCH À,PÉREZ-CANO F J,et al.Influence of hesperidin on the systemic and intestinal rat immune response[J].Nutrients,2017,9(6):580.
[46] POURHOSSEIN Z,QOTBI A A A,SEIDAVI A,et al.Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens[J].Animal Science Journal,2015,86(1):105-110.  
[47] TRIVEDI P J,ADAMS D H.Gut-liver immunity[J].Journal of Hepatology,2016,64(5):1187-1189.  
[48] PENG H,WISSE E,TIAN Z G.Liver natural killer cells:subsets and roles in liver immunity[J].Cellular & Molecular Immunology,2016,13(3):328-336.  
[49] LEI Y,HUANG T,SU M,et al.Expression and distribution of immunoglobulin G in the normal liver,hepatocarcinoma and postpartial hepatectomy liver[J].Laboratory Investigation,2014,94(11):1283-1295.  
[50] KRONBORG I J,KNOPF P M,BHATHAL P S,et al.Intrahepatic synthesis of immunoglobulin in liver disease[J].Liver,1982,2(4):385-392.
[51] SHEN H,ZHANG M N,KAITA K,et al.Expression of Fc fragment receptors of immunoglobulin G (FcγRs) in rat hepatic stellate cells[J].Digestive Diseases and Sciences,2005,50(1):181-187.  
[52] INAMINE T,SCHNABL B.Immunoglobulin a and liver diseases[J].Journal of Gastroenterology,2018,53(6):691-700.  
[53] PLANER J D,PENG Y Q,KAU A L,et al.Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice[J].Nature,2016,534(7606):263-266.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2