动物营养学报 >

2020 , Vol. 32 >Issue 1: 321 - 333

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.039

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

白藜芦醇对不同类型底物体外产气和发酵参数的影响及其代谢产物的研究

  • 吴万成 ,
  • 马涛 ,
  • 李文娟 ,
  • 刘娜 ,
  • 陈国顺 ,
  • 刁其玉
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 中国农业科学院饲料研究所, 农业农村部饲料生物技术重点实验室, 北京 100081
吴万成(1994-),男,甘肃兰州人,硕士研究生,从事动物遗传育种与繁殖学研究。E-mail:892177792@qq.com

收稿日期: 2019-07-12

  网络出版日期: 2020-01-19

基金资助

国家自然科学基金（41705129）；国家重点研发计划"反刍动物甲烷排放测算模型及基于宏基因组学的减排技术"（2016YFE0109000）

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Effects of Resveratrol on in Vitro Gas Production and Fermentation Parameters of Different Types of Substrates and Its Metabolites Research

  • WU Wancheng ,
  • MA Tao ,
  • LI Wenjuan ,
  • LIU Na ,
  • CHEN Guoshun ,
  • DIAO Qiyu
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2019-07-12

  Online published: 2020-01-19

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摘要

本试验旨在探索2种类型底物下添加不同水平白藜芦醇（RES）对体外产气和发酵参数的影响，为合理应用天然植物提取物调控反刍动物瘤胃发酵提供依据。选取3只体况良好的安装有永久性瘤胃瘘管的杜寒杂交肉用公羊作为瘤胃液供体。采用单因素试验设计，在2种类型底物[精粗比分别为68：32（高精料）和28：72（高粗料）]中分别添加0、7.7%、14.3%和25.0%（干物质基础）的RES进行体外产气试验。分别在体外发酵0、2、4、8、12、16、24 h时记录产气量，计算产气参数；在发酵24 h后测定发酵液pH和挥发性脂肪酸（VFA）浓度。在2种类型底物[精粗比分别为68：32（高精料）和28：72（高粗料）]条件下添加14.3%的RES进行RES体外降解试验，分别在发酵0、12和24 h时检测发酵残余物中RES及其代谢产物浓度。结果显示：1）随着发酵时间的延长，2种底物的产气量均增加。高精料条件下，随RES添加水平的升高，24 h产气量、产气速率均呈线性降低（P<0.05），但各产气参数在高粗料的各RES添加组间无显著差异（P>0.05）。2）高粗料条件下，随着RES添加水平的升高，发酵液pH无显著变化（P>0.05）；各RES添加组发酵液中乙酸摩尔比例以及总VFA浓度和乙酸/丙酸在发酵12和24 h时均显著低于未添加RES组（P<0.05），丙酸摩尔比例显著高于未添加RES组（P<0.05）；发酵液中丁酸摩尔比例在发酵12 h时表现为14.3%和25.0% RES组显著低于未添加RES组（P<0.05），在发酵24 h时表现为各RES添加组显著低于未添加RES组（P<0.05）。高精料条件下，随着RES添加水平的升高，在发酵12 h时，14.3%和25.0% RES组发酵液pH均显著高于未添加RES组（P<0.05）；在发酵24 h时，各RES添加组发酵液pH均显著高于未添加RES组（P<0.05）；各RES添加组发酵液乙酸摩尔比例在发酵12 h时均显著低于未添加RES组（P<0.05），在发酵24 h时14.3%和25.0% RES组显著低于未添加RES组（P<0.05）；各RES添加组发酵液丙酸摩尔比例在2个发酵时间点均显著高于未添加RES组（P<0.05）；各RES添加组发酵液丁酸摩尔比例以及总VFA浓度和乙酸/丙酸在2个发酵时间点均显著低于未添加RES组（P<0.05）。3）RES在2种类型底物条件下均发生了降解，且在高精料条件下表现出了更强的降解特性，主要降解产物为二氢白藜芦醇（DH-RES）。由本试验结果发现，底物精粗比会影响RES调控体外产气和发酵的效果，高添加水平的RES对体外瘤胃发酵具有一定抑制作用；RES可以在瘤胃环境中降解代谢，且RES及其代谢产物DH-RES在高精料条件下显示出更强的降解特性。

关键词： 白藜芦醇; 体外发酵; 挥发性脂肪酸; 二氢白藜芦醇

本文引用格式

吴万成 , 马涛 , 李文娟 , 刘娜 , 陈国顺 , 刁其玉 . 白藜芦醇对不同类型底物体外产气和发酵参数的影响及其代谢产物的研究[J]. 动物营养学报, 2020 , 32(1) : 321 -333 . DOI: 10.3969/j.issn.1006-267x.2020.01.039

Abstract

This experiment explored the effects of different supplemental levels of resveratrol (RES) on in vitro gas production and fermentation parameters under two types of substrates, with an aim to rationally apply natural plant extracts to regulate rumen fermentation of ruminants. Three rams were used as rumen fluid donors with rumen fistula. Rumen fluid was collected from three adult Dorper×thin-tailed Han crossbred male lambs fitted with permanent rumen cannulation. Using a single factor test design, 0, 7.7%, 14.3%, and 25.0% RES (dry matter basis) were added to the two substrates[forage to concentrate ratio was 28:72 (high forge) or 68:32 (high concentrate)], respectively, to carry out in vitro gas production test. At 0, 2, 4, 8, 12, 16 and 24 h of in vitro fermentation, the gas production was recorded and the gas production parameters were calculated, respectively; the fermentation liquid pH and volatile fatty acid (VFA) concentrations were determined at 24 h of fermentation. Under the conditions of two types of substrates[forage to concentrate ratio was 28:72 (high forge) or 68:32 (high concentrate)], 14.3% of RES was added for RES in vitro degradation test, and the concentrations of RES and its metabolites were detected in fermentation residues at 0, 12 and 24 h of fermentation, respectively. The results showed as follows:1) as the fermentation time prolonged, the gas production of both substrates increased. Under the high concentrate condition, the 24 h gas production and gas production rate decreased linearly with the RES supplemental level increasing (P<0.05). However, the gas production parameters were not significantly different among the adding RES groups under high forage condition (P>0.05). 2) Under the high forage condition, with the RES supplemental level increasing, there was no significant change in pH (P>0.05); the molar proportion of acetate, total VFA concentration and acetate/propionate in fermentation broth of each RES addition group were significantly lower than those of the unadded RES group at 12 and 24 h of fermentation (P<0.05), while the molar proportion of propionate was significantly higher than that of the unadded RES group (P<0.05); the molar proportion of butyrate in fermentation broth of the 14.3% and 25.0% RES groups was significantly lower than that of the unadded RES group at 12 h of fermentation (P<0.05), and it in each RES addition group was significantly lower than that of the unadded RES group at 24 h of fermentation (P<0.05). Under the high concentrate condition, with the RES supplemental level increasing, the fermentation broth pH of 14.3% and 25.0% RES groups was significantly higher than that of the unadded RES group at 12 h of fermentation (P<0.05), and it of each RES addition group was significantly higher than that of the RES group at 24 h of fermentation (P<0.05); the molar proportion of acetic acid in fermentation broth of each RES addition group was significantly lower than that of the unadded RES group at 12 h of fermentation (P<0.05), and it of 14.3% and 25.0% RES groups were significantly lower than that of the unadded RES group at 24 h of fermentation (P<0.05); the molar proportion of propionic acid in fermentation of each RES addition group was significantly higher than that of the unadded RES group at the two fermentation time points (P<0.05); the molar proportion of butyric acid, the total VFA concentration and acetic acid/propionic acid in fermentation of each RES addition group were significantly lower than those of the unadded RES group at the two fermentation time points (P<0.05). 3) RES was degraded in both two types of substrate conditions and more degradable in high concentrate condition. Its main metabolite was dihydro-resveratrol (DH-RES). The above results indicate that the forage to concentrate ratio of substrate affects the regulation effect of RES on gas production and fermentation, and high supplemental level of RES has an inhibitory effect on rumen fermentation. RES can be degraded and metabolized in the rumen environment, and RES and its metabolite DH-RES show stronger degradation characteristics under the high concentrate condition.

Key words： resveratrol; in vitro fermentation; volatile fatty acid; dihydro-resveratrol

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