动物营养学报 >

2020 , Vol. 32 >Issue 1: 397 - 404

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.046

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

天然蜂粮和蜂花粉对小鼠免疫功能的调节作用

  • 李震 ,
  • 刘志勇 ,
  • 李龙雪 ,
  • 舒坤 ,
  • 刘光楠 ,
  • 张祖芸 ,
  • 颜伟玉 ,
  • 曾志将
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  • 1. 江西农业大学蜜蜂研究所, 南昌 330045;
    2. 江西中医药大学实验动物科技中心, 南昌 330006;
    3. 江西省石城县农业农村局, 石城 342700;
    4. 云南省农业科学院蚕桑蜜蜂研究所, 蒙自 661100
李震(1995-),男,安徽六安人,硕士研究生,从事蜂产品研究。E-mail:zhenli1995@sina.com

收稿日期: 2019-07-02

  网络出版日期: 2020-01-19

基金资助

国家现代蜂产业技术体系建设专项（CARS-44-kxj15）

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Regulating Effect of Natural Bee Bead and Bee Pollen on Immune Function of Mice

  • LI Zhen ,
  • LIU Zhiyong ,
  • LI Longxue ,
  • SHU Kun ,
  • LIU Guangnan ,
  • ZHANG Zuyun ,
  • YAN Weiyu ,
  • ZENG Zhijiang
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  • 1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Laboratory Animal Science and Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China;
    3. Agricultural and Rural Bureau of Shicheng County, Jiangxi Province, Shicheng 342700, China;
    4. Sericultural and Apicultural Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661100, China

Received date: 2019-07-02

  Online published: 2020-01-19

Supported by

 

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摘要

本试验旨在研究天然蜂粮和蜂花粉对小鼠免疫功能的调节作用。以昆明小鼠作为试验动物，设6个试验组（天然蜂粮和蜂花粉均设低、中、高3个剂量组）和1个空白对照组，每组32只小鼠。参照人推荐食用蜂花粉剂量400 mg/（d·kg BW），将低、中和高3个剂量分别设定为80、400、800 mg/（d·kg BW）。连续灌胃小鼠30 d天然蜂粮和蜂花粉后，对小鼠做如下处理：1）进行体重称量和脾脏、胸腺指数测定；2）进行自然杀伤（NK）细胞活性测定；3）进行刀豆蛋白A（ConA）诱导的小鼠脾淋巴细胞转化试验；4）进行碳廓清试验；5）进行抗体生成细胞检测。结果显示：相同周龄时，各组小鼠体重无显著差异（P>0.05）。与空白对照组相比，蜂粮中剂量组和3个蜂花粉剂量组小鼠的NK细胞活性均显著升高（P<0.05）；6个试验组小鼠的吞噬指数和脾淋巴细胞增殖能力与空白对照组无显著差异（P>0.05）；与3个蜂花粉剂量组相比，蜂粮低剂量组小鼠胸腺指数显著升高（P<0.05）；6个试验组小鼠的溶血空斑数均较空白对照组显著增加（P<0.05）；与3个蜂花粉剂量组相比，蜂粮高剂量组小鼠溶血空斑数显著增加（P<0.05）。由此得出，不同剂量天然蜂粮与蜂花粉对小鼠的生长均无不良影响，且二者均能提高NK细胞活性，并可提高体液免疫功能，而且天然蜂粮还能够刺激胸腺发育。综合来看，天然蜂粮调节免疫的功效要优于蜂花粉。

关键词： 天然蜂粮; 蜂花粉; 小鼠; 免疫功能

本文引用格式

李震 , 刘志勇 , 李龙雪 , 舒坤 , 刘光楠 , 张祖芸 , 颜伟玉 , 曾志将 . 天然蜂粮和蜂花粉对小鼠免疫功能的调节作用[J]. 动物营养学报, 2020 , 32(1) : 397 -404 . DOI: 10.3969/j.issn.1006-267x.2020.01.046

Abstract

The aim of this study was to study the regulating effect of natural bee bead and bee pollen on the immune function of mice. Kunming mice were used as experimental animals and divided into one blank control group and six experimental groups, and each group had 32 mice. The six experimental groups included three doses (low, middle and high doses) of natural bee bread groups and three doses (low, middle and high doses) of bee pollen groups. Referring to the human daily recommended feeding dosage[400 mg/(d·kg BW)] of bee pollen, low, middle and high doses were set as 80, 400 and 800 mg/(d·kg BW), respectively. After intragastric administration natural bee bread and bee pollen for 30 days, the mice were treated as follows:1) body weight, spleen and thymus indices were measured. 2) The natural killer (NK) cell activity was detected. 3) Concanavalin A (ConA)-induced transformation of mouse spleen lymphocytes experimental was performed. 4) Carbon clearance experiment was carried out. 5) Antibody producing cells were detected. The results showed as follows:the body weight among all groups in the same week age was no significant difference (P>0.05). The NK cell activity of mice in the middle dose bee bread group and the three bee pollen dose groups was significantly increased compared with the blank control group (P<0.05). There was no significant difference in the phagocytic index and the splenic lymphocyte proliferation capacity of mice in the six experimental groups compared with the blank control group (P>0.05). The thymus index of mice in low dose bee bread group was significantly increased compared with the three bee pollen dose groups (P<0.05). The number of hemolytic plaques of mice in the six experimental groups was significantly increased compared with the blank control group (P<0.05). Moreover, the number of hemolytic plaques of mice in the high dose bee bread group was significantly higher than that in the three bee pollen dose groups (P<0.05). It suggests that different doses of natural bee bread and bee pollen have no harmful effects on growth on mice, but both of them can increase the NK cell activity, enhance the humoral immune function, and the natural bee bread also can stimulate the development of thymus of mice. Overall, the effect of natural bee bread on regulating immune function is superior to bee pollen.

Key words： natural bee bread; bee pollen; mice; immune function

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