鸭油甘油二酯与维生素D3协同对肥胖SD大鼠器官指数、血液25羟基维生素D3含量、胫骨发育指标及肝脏维生素D受体和过氧化酶体增殖物激活受体&alpha;基因表达量的影响

刘亚楠; 王宝维; 葛文华; 张名爱; 孙京新; 孔敏

doi:10.3969/j.issn.1006-267x.2020.01.055

动物营养学报 >

2020 , Vol. 32 >Issue 1: 472 - 480

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.01.055

研究简报 SHORT COMMUNICATION

鸭油甘油二酯与维生素D₃协同对肥胖SD大鼠器官指数、血液25羟基维生素D₃含量、胫骨发育指标及肝脏维生素D受体和过氧化酶体增殖物激活受体α基因表达量的影响

刘亚楠 ,
王宝维 ,
葛文华 ,
张名爱 ,
孙京新 ,
孔敏

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1. 青岛农业大学食品科学与工程学院, 青岛 266109;
2. 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109

刘亚楠(1995-),女,山东荣成人,硕士研究生,研究方向为营养与保健。E-mail:657347874@qq.com

收稿日期: 2019-07-22

网络出版日期: 2020-01-19

基金资助

国家水禽产业技术体系专项基金（CARS-43-11）；山东省2018年度农业重大应用技术创新项目

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Effects of Combination of Duck Oil Diglyceride and Vitamin D₃ on Organ Indexes, Blood 25 Hydroxyl Vitamin D₃ Content, Tibia Development Indexes and Liver Vitamin D Receptor and Peroxisome Proliferators-Activated Receptors α Gene Expressions of Obese SD Rats

LIU Yanan ,
WANG Baowei ,
GE Wenhua ,
ZHANG Ming ,
SUN Jingxin ,
KONG Min

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1. Department of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
2. National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2019-07-22

Online published: 2020-01-19

Supported by

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摘要

本试验旨在研究鸭油甘油二酯与维生素D₃协同对肥胖SD大鼠器官指数、血液25羟基维生素D₃含量、胫骨发育指标及肝脏维生素D受体（VDR）和过氧化酶体增殖物激活受体α（PPARα）基因表达量的影响，以探索鸭油甘油二酯与维生素D₃的协同作用。试验选取60只雄性SD大鼠，随机分为4个组（对照组、肥胖模型组、维生素D₃组、鸭油甘油二酯+维生素D₃组），每组3个重复，每个重复5只。1~3周，对照组饲喂基础饲粮，其他各组饲喂高脂饲粮进行肥胖造模；4~6周，造模成功后，对照组饲喂基础饲粮，肥胖模型组饲喂高脂饲粮，维生素D₃组、鸭油甘油二酯﹢维生素D₃组在高脂饲粮中添加12.5 μg/kg维生素D₃，鸭油甘油二酯+维生素D₃组灌胃1 mL/（kg·BW d）鸭油甘油二酯，其他各组灌胃等量的蒸馏水。试验期6周。结果表明，与肥胖模型组相比：1）鸭油甘油二酯与维生素D协同能够显著降低肝脏指数（P<0.05），对心脏、脾脏、肾脏指数无显著影响（P>0.05）；2）鸭油甘油二酯与维生素D₃协同能够显著升高1、2、3周末的血液25羟基维生素D₃含量（P<0.05）；3）鸭油甘油二酯与维生素D₃协同能够显著提高胫骨的骨密度、骨强度（P<0.05），显著降低血液骨源性碱性磷酸酶活性（P<0.05）；4）鸭油甘油二酯与维生素D₃协同能够显著上调肝脏VDR和PPARα基因表达量（P<0.05）。由此可见，鸭油甘油二酯与维生素D₃协同能够降低肥胖大鼠肝脏指数，提高血液25羟基维生素D₃含量，上调肝脏VDR和PPARα基因表达量，促进胫骨发育。

关键词： 鸭油甘油二酯; 维生素D₃; 胫骨发育; 维生素D受体; PPARα; 肥胖大鼠

本文引用格式

刘亚楠 , 王宝维 , 葛文华 , 张名爱 , 孙京新 , 孔敏 . 鸭油甘油二酯与维生素D₃协同对肥胖SD大鼠器官指数、血液25羟基维生素D₃含量、胫骨发育指标及肝脏维生素D受体和过氧化酶体增殖物激活受体α基因表达量的影响[J]. 动物营养学报, 2020 , 32(1) : 472 -480 . DOI: 10.3969/j.issn.1006-267x.2020.01.055

Abstract

This experiment was conducted to study the effects of duck oil diglyceride and vitamin D₃ coordination on organ indexes, blood 25 hydroxyl vitamin D₃ content, tibia development indexes and liver vitamin D receptor (VDR) and peroxisome proliferators-activated receptors α (PPARα) gene expressions of obese SD rats, to explore the synergistic relationship between duck oil diglyceride and vitamin D₃. Sixty male SD rats were randomly assigned into 4 groups (control group, obese model group, vitamin D₃ group and duck oil diglyceride+vitamin D₃ group) with 3 replicates per group and 5 rats per replicate. During 1 to 3 weeks, rats in the control group were fed a basal diet, and the others in other groups were fed high-fat diets for obesity modeling. During 4 to 6 weeks, after successful modeling, rats in the control group were fed a basal diet, rats in the obese model group were fed a high-fat diet, rats in vitamin D₃ group and duck oil diglyceride+vitamin D₃ group were fed high-fat diet supplemented 12.5 μg/kg vitamin D₃, rats in duck oil diglyceride+vitamin D₃ group were given 1 mL/(kg·BW d) duck oil diglyceride by gavage, and rats in other groups were given the same amount of distilled water by gavage. The experiment lasted for 6 weeks. The results showed that compared with the obese model group:1) the combination of duck oil diacylglycerol and vitamin D₃ could significantly reduce the liver index (P<0.05), but had no significant effect on the indexes of heart, spleen and kidney (P>0.05); 2) the combination of duck oil diglyceride and vitamin D₃ could significantly increase the blood 25 hydroxy vitamin D₃ content (P<0.05); 3) the combination of duck oil diglyceride and vitamin D could significantly increase the bone density and bone strength in tibia (P<0.05), and significantly decreased the blood bone derived alkaline phosphatase activity (P<0.05); 4) the combination of duck oil diglyceride and vitamin D₃ could significantly increase the liver VDR and PPARα gene expressions (P<0.05). In conclusion, the combination of duck oil diglyceride and vitamin D₃ can decrease the liver index of obese rats, increase the blood 25 hydroxy vitamin D₃ content, increase the liver VDR and PPARα gene expressions, and promote the development of tibia.

Key words： duck oil diglyceride; vitamin D₃; tibia development; vitamin D receptor; PPARα; obese rats

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