猪营养与饲料 SWINE NUTRITION AND FEED

地衣芽孢杆菌对脂多糖应激仔猪肠道形态、肠黏膜抗氧化能力和免疫力的影响

  • 宦海琳 ,
  • 张干 ,
  • 徐祝华 ,
  • 闫俊书 ,
  • 林勇 ,
  • 徐小明 ,
  • 周维仁
展开
  • 1. 江苏省农业科学院畜牧研究所, 南京 210014;
    2. 江苏省农业种质资源保护与利用平台, 南京 210014;
    3. 泰兴瑞泰化工有限公司, 泰州 225453
宦海琳(1981-),女,江苏扬州人,副研究员,硕士,从事益生菌与动物肠道健康研究。E-mail:huanhl@126.com

收稿日期: 2019-07-29

  网络出版日期: 2020-02-21

基金资助

江苏省农业自主创新基金(CX135040)

Effects of Bacillus licheniformis on Intestinal Morphology, Intestinal Mucosal Antioxidant Capacity and Intestinal Immunity of Lipopolysaccharide-Stressed Piglets

  • HUAN Hailin ,
  • ZHANG Gan ,
  • XU Zhuhua ,
  • YAN Junshu ,
  • LIN Yong ,
  • XU Xiaoming ,
  • ZHOU Weiren
Expand
  • 1. Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
    2. Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, China;
    3. Taixing Ruitai Chemical Co., Ltd., Taizhou 225453, China

Received date: 2019-07-29

  Online published: 2020-02-21

摘要

本试验旨在研究地衣芽孢杆菌对脂多糖(LPS)应激仔猪肠道形态、肠黏膜抗氧化能力和免疫力的影响。试验选用35日龄、平均体重为(10.0±0.5)kg的苏山猪90头,随机分成3组(对照组、LPS组和BL+LPS组),每组3个重复,每个重复10头仔猪。其中,对照组和LPS组仔猪均饲喂基础饲粮;BL+LPS组仔猪饲喂添加500 mg/kg地衣芽孢杆菌的基础饲粮。预试期3 d,正试期21 d,于正试期第21天从每个重复中选2头仔猪腹腔注射LPS(LPS组和BL+LPS组)或等量的灭菌生理盐水(对照组),注射24 h后屠宰取样。结果显示:1)与对照组相比,LPS应激显著降低了仔猪十二指肠二胺氧化酶(DAO)、空肠一氧化氮合成酶(NOS)活性及空肠一氧化氮(NO)含量(P<0.05),显著提高了血浆中NO含量和NOS活性(P<0.05);显著降低了空肠绒毛高度和绒隐比、回肠绒隐比(P<0.05);显著降低了十二指肠、空肠和回肠谷胱甘肽过氧化物酶(GSH-Px)、十二指肠和回肠超氧化物歧化酶(SOD)活性(P<0.05);显著降低了空肠上皮细胞中淋巴细胞、空肠和回肠上皮细胞中杯状细胞数量(P<0.05);显著降低了血清免疫球蛋白G(IgG)含量,提高了回肠黏膜白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)含量(P<0.05)。2)与LPS组相比,饲粮添加地衣芽孢杆菌显著提高了LPS应激仔猪回肠DAO和NOS活性(P<0.05);显著提高了空肠黏膜GSH-Px活性显著提高(P<0.05),显著降低了十二指肠黏膜丙二醛(MDA)含量(P<0.05);显著提高了血清IgG含量(P<0.05)。由此可见,LPS应激导致仔猪肠黏膜损伤,在饲粮中添加地衣芽孢杆菌可提高LPS应激仔猪的肠黏膜抗氧化能力和免疫力,一定程度上促进肠道形态的修复,减缓LPS应激导致的肠道损伤。

本文引用格式

宦海琳 , 张干 , 徐祝华 , 闫俊书 , 林勇 , 徐小明 , 周维仁 . 地衣芽孢杆菌对脂多糖应激仔猪肠道形态、肠黏膜抗氧化能力和免疫力的影响[J]. 动物营养学报, 2020 , 32(2) : 586 -595 . DOI: 10.3969/j.issn.1006-267x.2020.02.014

Abstract

The aim of this study was to investigate the effects of Bacillus licheniformis on intestinal morphology, intestinal mucosal antioxidant capacity and intestinal immunity of lipopolysaccharide (LPS)-stressed piglets. The experiment used 90 heads of Sushan pigs of 35 days old and weighing about (10.0±0.5) kg, they were randomly divided into 3 groups (control group, LPS group and BL+LPS group) with 3 repetitions in each group and 10 repetitions each. The pigs in control group and LPS group were fed a basal diet, while the pigs in BL+LPS group were fed the basal diet supplemented with 500 mg/kg Bacillus licheniformis (1.0×1010 CFU/g). There was a pretrial period of 3-day followed by an experimental period of 21-day. Two piglets were selected from each group and injected intraperitoneally with 150 μg/kg BW LPS (LPS group and BL+LPS group) or an equivalent amount of sterile saline (control group) at day 21 of experimental period. After 24 h of injection, the piglets were slaughtered and sampled. The results showed as follows:1) compared with the control group, LPS stress significantly reduced the activities of diamine oxidase (DAO) in duodenum, nitric oxide synthetase (NOS) in jejunum and the content of nitric oxide (NO) in jejunum of weaned piglets (P<0.05), significantly increased the NO content and NOS activity in plasma (P<0.05), significantly decreased the villus height (VH) and VH/crypt depth (V/C) of jejunum and the V/C of ileum (P<0.05), significantly decreased the activities of glutathione peroxidase (GSH-Px) in duodenum, jejunum and ileum and superoxide dismutase (SOD) in ileum (P<0.05), and significantly reduce the counts of lymphocytes in jejunal epithelial cells and goblet cells in jejunal and ileal epithelial cells (P<0.05). In addition, LPS stress significantly decreased serum immunoglobulin (IgG) content and significantly increased interleukin-6 (IL-6) and interleukin-1β (IL-1β) contents in ileal mucosa (P<0.05). 2) Compared with the LPS group, adding Bacillus licheniformis in the diet significantly increased the activities of DAO and NOS in ileum (P<0.05), improved jejunum VH and V/C (P>0.05), significantly increased the activity of GSH-Px in jejunal mucosa (P<0.05), significantly decreased the content of MDA in duodenal mucosa (P<0.05), and significantly increased the content of IgG in serum of LPS-stressed piglets (P<0.05). The results indicate that LPS stress causes intestinal mucosal injury in piglets. Adding Bacillus licheniformis in the diet can improve the antioxidant capacity and immunity of intestinal mucosa of LPS-stressed piglets, promote the repair of intestinal morphology to a certain extent, and alleviate the intestinal damage caused by LPS stress.

参考文献

[1] WANG H L,SHI M,XU X,et al.Effects of flavomycin,Bacillus licheniformis and enramycin on performance,nutrient digestibility,gut morphology and the intestinal microflora of broilers[J].The Journal of Poultry Science,2016,53(2):128-135.  
[2] WANG Y,DU W,LEI K,et al.Effects of Dietary Bacillus licheniformis on gut physical barrier,immunity,and reproductive hormones of laying hens[J].Probiotics and Antimicrobial Proteins,2017,9(3):292-299.  
[3] YANG J J,KUN Q,WU D,et al.Effects of different proportions of two Bacillus sp. on the growth performance,small intestinal morphology,caecal microbiota and plasma biochemical profile of Chinese Huainan Partridge shank chickens[J].Journal of Integrative Agriculture,2017,16(6):1383-1392.  
[4] LAN R X,TRAN H,KIM I.Effects of probiotic supplementation in different nutrient density diets on growth performance,nutrient digestibility,blood profiles,fecal microflora and noxious gas emission in weaning pig[J].Journal of the Science of Food and Agriculture,2017,97(4):1335-1341.  
[5] ZONG X,WANG T,LU Z,et al.Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance,blood indexes,and intestinal barrier function of weanling piglets[J].Livestock Science,2018,220:137-142.
[6] ZHU C,WU Y P,JIANG Z Y,et al.Dietary soy isoflavone attenuated growth performance and intestinal barrier functions in weaned piglets challenged with lipopolysaccharide[J].International Immunopharmacology,2015,28(1):288-294.  
[7] 康保聚,陈家顺,金顺顺,等.血根碱对脂多糖免疫应激仔猪生长性能、免疫功能及肠道健康的影响[J].动物营养学报,2019,31(9):4251-4261.
[8] 肖定福.壳聚糖对仔猪生长、肠道屏障和免疫的影响及其机理研究[D].博士学位论文.长沙:湖南农业大学,2011.
[9] WU Q J,WANG Y Q,QI Y X.The protective effect of procyanidin against LPS-induced acute gut injury by the regulations of oxidative state[J].SpringerPlus,2016,5:1645.
[10] LI Y,ZHANG H,CHEN Y P,et al.Bacillus amyloliquefaciens supplementation alleviates immunological stress and intestinal damage in lipopolysaccharide-challenged broilers[J].Animal Feed Science and Technology,2015,208:119-131.
[11] LEI K,LI Y L,YU D Y,et al.Influence of dietary inclusion of Bacillus licheniformis on laying performance,egg quality,antioxidant enzyme activities,and intestinal barrier function of laying hens[J].Poultry Science,2013,92(9):2389-2395.  
[12] ZHANG Q,TAN B P,MAI K S,et al.Dietary administration of Bacillus (B. licheniformis and B. subtilis) and isomaltooligosaccharide influences the intestinal microflora,immunological parameters and resistance against Vibrio alginolyticus in shrimp,Penaeus japonicus (Decapoda:Penaeidae)[J].Aquaculture Research,2011,42(7):943-952.  
[13] YANG G Y,ZHU Y H,ZHANG W,et al.Influence of orally fed a select mixture of Bacillus probiotics on intestinal T-cell migration in weaned MUC4 resistant pigs following Escherichia coli challenge[J].Veterinary Research,2016,47:71.
[14] KONG W G,HUANG C,TANG Y,et al.Effect of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp (Ctenopharyngodon idella)[J].Scientific Reports,2017,7:1588.
[15] HA N,GONÇALVES A F N,SOUSA L C,et al.Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation[J].Aquaculture International,2017,25(2):755-776.  
[16] GADDE U D,OH S,LEE Y,et al.Dietary Bacillus subtilis-based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens[J].Research in Veterinary Science,2017,114:236-243.
[17] YA'ACOV A B,LICHTENSTEIN Y,ZOLOTAROV L,et al.The gut microbiome as a target for regulatory T cell-based immunotherapy:induction of regulatory lymphocytes by oral administration of anti-LPS enriched colostrum alleviates immune mediated colitis[J].BMC Gastroenterology,2015,15:154.
[18] 李军亮,杨奇慧,谭北平,等.低鱼粉饲料添加枯草芽孢杆菌对珍珠龙胆石斑鱼幼鱼生长、消化酶活性、抗氧化酶活性及其mRNA表达的影响[J].水产学报,2019,43(4):1126-1137.
[19] DA SILVA E O,GEREZ J R,HOHMANN M S N,et al.Phytic acid decreases oxidative stress and intestinal lesions induced by Fumonisin B1 and deoxynivalenol in intestinal explants of pigs[J]. Toxins,2019,11:18.
[20] CHEN Z C,ZHAO S F,LIU Y,et al.Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot,Scophthalmus maximus L[J].Aquaculture Research,2018,49(12):3804-3816.  
[21] HUANG X,KONG G Q,LI Y,et al.Decitabine and 5-azacitidine both alleviate LPS induced ARDS through anti-inflammatory/antioxidant activity and protection of glycocalyx and inhibition of MAPK pathways in mice[J].Biomedicine & Pharmacotherapy,2016,84:447-453.
[22] SONG Z H,TONG G,XIAO K,et al.L-cysteine protects intestinal integrity,attenuates intestinal inflammation and oxidant stress,and modulates NF-κB and Nrf2 pathways in weaned piglets after LPS challenge[J].Innate Immunity,2016,22(3):152-161.  
[23] CAO S T,ZHANG Q H,WANG C C,et al.LPS challenge increased intestinal permeability,disrupted mitochondrial function and triggered mitophagy of piglets[J].Innate Immunity,2018,24(4):221-230.  
[24] 张甜甜,殷海成,黄巍.枯草芽孢杆菌肽聚糖对β-伴大豆球蛋白诱导的鲤幼鱼肠上皮细胞损伤的保护作用[J].水产学报,2018,42(4):495-502.
[25] ZHANG L L,BAI K W,ZHANG J F,et al.Dietary effects of Bacillus subtilis fmbj on the antioxidant capacity of broilers at an early age[J].Poultry Science,2017,96(10):3564-3573.  
[26] BORGHESI L,MILCAREK C.From B cell to plasma cell:regulation of V(D)J recombination and antibody secretion[J].Immunologic Research,2006,36(1/2/3):27-32.
[27] CARR M W,ROTH S J,LUTHER E,et al.Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant[J].Proceedings of the National Academy of Sciences of the United States of America,1994,91(9):3652-3656.  
[28] HAN J M,LEE E K,GONG S Y,et al.Sparassis crispa exerts anti-inflammatory activity via suppression of TLR-mediated NF-κB and MAPK signaling pathways in LPS-induced RAW264.7 macrophage cells[J].Journal of Ethnopharmacology,2019,231:10-18.
[29] GIRI S S,SEN S S,JUN J W,et al.Role of Bacillus licheniformis VS16-derived biosurfactant in mediating immune responses in carp rohu and its application to the food industry[J].Frontiers in Microbiology,2017,8:514.
[30] ZHEN W R,SHAO Y J,GONG X Y,et al.Effect of dietary Bacillus coagulans supplementation on growth performance and immune responses of broiler chickens challenged by Salmonella enteritidis[J].Poultry Science,2018,97(8):2654-2666.  
[31] MOU C X,ZHU L Q,XING X P,et al.Immune responses induced by recombinant Bacillus subtilis expressing the spike protein of transmissible gastroenteritis virus in pigs[J].Antiviral Research,2016,131:74-84.
[32] WU Z,PAN D D,GUO X X,et al.Structure and anti-inflammatory capacity of peptidoglycan from Lactobacillus acidophilus in RAW-264.7 cells[J].Carbohydrate Polymers,2013,96(2):466-473.  
文章导航

/