动物营养学报 >

2020 , Vol. 32 >Issue 2: 596 - 604

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.015

猪营养与饲料 SWINE NUTRITION AND FEED

长白公猪血清和精浆中元素含量对精液品质的影响

  • 吴英慧 ,
  • 王超 ,
  • 谭家健 ,
  • 孙海清 ,
  • 彭健
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  • 1. 华中农业大学动物科技学院, 武汉 430070;
    2. 广西扬翔股份有限公司, 贵港 537000;
    3. 生猪健康养殖协同中心, 武汉 430070
吴英慧(1993-),女,山东菏泽人,博士研究生,动物营养与饲料科学专业。E-mail:academy_wuyinghui@163.com

收稿日期: 2019-07-24

  网络出版日期: 2020-02-21

基金资助

国家重点研发项目（2017YFD0502004）；国家现代产业技术体系（CARS-36）

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Effects of Element Contents in Serum and Seminal Plasma on Semen Quality of Landrace Boars

  • WU Yinghui ,
  • WANG Chao ,
  • TAN Jiajian ,
  • SUN Haiqing ,
  • PENG Jian
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  • 1. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. Guangxi YangXiang Joint Stock Co., Ltd., Guigang 537000, China;
    3. The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China

Received date: 2019-07-24

  Online published: 2020-02-21

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摘要

本试验旨在研究不同精液品质特征长白公猪血清和精浆中元素含量的差异，分析血清和精浆中元素含量对精液品质的影响。基于107头长白公猪的1 402次精液品质记录，将公猪群按照精液可利用率划分为3组：低利用率组（利用率<60%，n=21）、中等利用率组（60%≤利用率≤80%，n=27）和高利用率组（利用率>80%，n=59）。采集每头长白公猪的血清和精浆样品，采用电感耦合等离子体质谱法检测血清和精浆中营养元素和毒性元素含量。结果表明：1）低利用率组长白公猪的有效精子数和精子活力显著低于中等利用率和高利用率组（P<0.01），精子畸形率显著高于中等利用率和高利用率组（P<0.01）。2）不同利用率组间长白公猪的血清和精浆元素含量无显著差异（P>0.05）；但在血清和精浆元素含量与精液品质参数相关性分析中发现，精浆铅元素含量与精子活力呈显著负相关（P<0.05），与精子畸形率呈显著正相关（P<0.05）。3）对精浆铅含量分组进一步分析发现，精浆铅含量为0 μg/L时长白公猪的精子畸形率显著低于精浆铅含量>10.0 μg/L时（P<0.05），精子畸形率降低约6.11%。总的来说，长白公猪精浆中毒性元素铅的存在会通过损害精子活力和形态，影响公猪精液品质。

关键词： 长白公猪; 精液品质; 营养元素; 毒性元素

本文引用格式

吴英慧 , 王超 , 谭家健 , 孙海清 , 彭健 . 长白公猪血清和精浆中元素含量对精液品质的影响[J]. 动物营养学报, 2020 , 32(2) : 596 -604 . DOI: 10.3969/j.issn.1006-267x.2020.02.015

Abstract

This experiment was conducted to investigate the differences of element contents in serum and seminal plasma of Landrace boars with different semen quality characteristics, and analyze the effects of element contents in serum and seminal plasma on semen quality. Based on 1 402 semen quality records of 107 Landrace boars, boars were allocated into 3 groups according to semen utilization rate:low utilization group (utilization was less than 60%,n=21), medium utilization group (utilization was 60% to 80%,n=27) and high utilization group (utilization was more than 80%,n=59). Serum and seminal plasma samples of Landrace boars were collected, and nutrient and toxic element contents in serum and seminal plasma were analyzed using inductively coupled plasma mass spectrometry. The results showed as follows:1) functional sperm number and sperm motility of Landrace boars in low utilization group were significantly lower than those in middle and high utilization group (P<0.01), and abnormal sperm rate was significantly higher than that in middle and high utilization group (P<0.01). 2) There were no significant differences in serum and seminal plasma element contents of Landrace boars between different utilization groups (P>0.05). While seminal plasma lead (Pb) element content was significantly negatively correlated with sperm motility (P<0.05), and significantly positively correlated with abnormal sperm rate by correlation analysis between serum and seminal plasma element contents and semen quality parameters (P<0.05). 3) Further analysis of seminal plasma Pb element content grouping showed that abnormal sperm rate of Landrace boars as 0 μg/L seminal plasma Pb element content was significantly lower than that as >10.0 μg/L seminal plasma Pb element content (P<0.05), and abnormal sperm rate decreased about 6.11%. In conclusion, existence of seminal plasma toxic Pb element may affect semen quality by impairing sperm motility and morphology of Landrace boars.

Key words： Landrace boar; semen quality; nutrient elements; toxic elements

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