不同水平脱脂米糠部分替代玉米对苏淮猪氧化还原状态和胆固醇代谢的影响

高芳芳; 李平华; 郑卫江; 黄瑞华; 姚文

doi:10.3969/j.issn.1006-267x.2020.02.018

动物营养学报 >

2020 , Vol. 32 >Issue 2: 626 - 635

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.018

猪营养与饲料 SWINE NUTRITION AND FEED

不同水平脱脂米糠部分替代玉米对苏淮猪氧化还原状态和胆固醇代谢的影响

高芳芳 ,
李平华 ,
郑卫江 ,
黄瑞华 ,
姚文

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1. 南京农业大学消化道微生物实验室, 江苏省消化道营养与动物健康重点实验室, 南京 210095;
2. 南京农业大学养猪研究所, 南京 210095;
3. 南京农业大学淮安研究院, 淮安 223005

高芳芳(1993-),女,山西晋城人,硕士研究生,研究方向为动物营养与消化道微生物。E-mail:1365005807@qq.com

收稿日期: 2019-07-29

网络出版日期: 2020-02-21

基金资助

江苏现代农业（生猪）产业技术体系营养调控创新团队（JATS[2018]287）

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Effects of Partial Replacement of Corn with Different Levels of Defatted Rice Bran on Redox State and Cholesterol Metabolism of Suhuai Pigs

GAO Fangfang ,
LI Pinghua ,
ZHENG Weijiang ,
HUANG Ruihua ,
YAO Wen

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1. Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Swine Science, Nanjing Agricultural University, Nanjing 210095, China;
3. Huai'an Academy of Nanjing Agricultural University, Huai'an 223005, China

Received date: 2019-07-29

Online published: 2020-02-21

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摘要

本试验旨在探讨不同水平脱脂米糠部分替代玉米对苏淮阉公猪氧化还原状态和胆固醇代谢的影响。试验选用21头体重[（62.90±1.12）kg]相近的健康苏淮阉公猪，随机分为对照组（试验Ⅰ组）、试验Ⅱ组和试验Ⅲ组，每组7个重复，每个重复1头猪。试验Ⅰ组猪饲喂基础饲粮，试验Ⅱ组和试验Ⅲ组猪分别以14%和28%的脱脂米糠部分替代基础饲粮中的玉米，并使用豆粕、麸皮和豆油等物质调平基础饲粮与试验饲粮的消化能、粗蛋白质和氨基酸水平。预试期10 d，正试期28 d。结果表明：1）试验Ⅱ组和试验Ⅲ组苏淮猪血清丙二醛（MDA）含量均显著低于试验Ⅰ组（P<0.05）；与试验Ⅰ组和试验Ⅲ组相比，试验Ⅱ组苏淮猪血清过氧化氢酶（CAT）活性显著升高（P<0.05）；试验Ⅲ组苏淮猪血清铜锌超氧化物歧化酶（CuZn-SOD）活性最高，且与试验Ⅰ组相比差异显著（P<0.05）；试验Ⅲ组苏淮猪肝脏还原型谷胱甘肽（GSH）含量以及还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）显著高于试验Ⅰ组（P<0.05）。2）与试验Ⅰ组相比，试验Ⅲ组苏淮猪血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）含量显著升高（P<0.05）；各组间肝脏TC和甘油三酯（TG）含量差异均不显著（P>0.05）。3）脱脂米糠部分替代饲粮玉米对苏淮猪肝脏胆固醇-7α-羟化酶1（CYP7α1）和胆固醇-27α-羟化酶1（CYP27α1）mRNA表达量均无显著影响（P>0.05）；试验Ⅱ组和试验Ⅲ组苏淮猪肝脏3-羟-3-甲基戊二酸单酰辅酶A还原酶（HMGCR）、固醇调节原件结合蛋白-2（SREBP-2）和清道夫受体BI（SR-BI）的mRNA表达量显著低于试验Ⅰ组（P<0.05）；此外，试验Ⅲ组苏淮猪肝脏低密度脂蛋白受体（LDLR）mRNA表达量最低，且与试验Ⅰ组相比差异显著（P<0.05）。4）将苏淮猪血清TC和LDL-C含量分别与血清和肝脏氧化标记物及抗氧化酶做相关性分析表明，同一个体血清TC含量与肝脏GSH含量以及GSH/GSSG呈显著正相关（P<0.05），而血清LDL-C含量与肝脏GSH含量以及GSH/GSSG极显著正相关（P<0.01）。综上所述，在本试验条件下，脱脂米糠部分替代饲粮中玉米可部分改变苏淮猪血清和肝脏氧化还原相关酶的活性，并在一定程度上影响机体胆固醇代谢和肝脏胆固醇代谢相关基因的表达。

关键词： 脱脂米糠; 苏淮猪; 氧化标记物; 抗氧化酶; 胆固醇代谢

本文引用格式

高芳芳 , 李平华 , 郑卫江 , 黄瑞华 , 姚文 . 不同水平脱脂米糠部分替代玉米对苏淮猪氧化还原状态和胆固醇代谢的影响[J]. 动物营养学报, 2020 , 32(2) : 626 -635 . DOI: 10.3969/j.issn.1006-267x.2020.02.018

Abstract

The purpose of this study was to investigate the effects of partial replacement of corn with different levels of defatted rice bran on redox state and cholesterol metabolism of Suhuai castrated boars. A total of 21 healthy Suhuai castrated boars with similar body weight of (62.90±1.12) kg were randomly divided into control group (test group Ⅰ), test group Ⅱ and test group Ⅲ, with 7 replicates per group and 1 pig per replicate. Pigs in the test group Ⅰ were fed a basal diet, and those in the test group Ⅱ and test group Ⅲ were fed the basal diets replaced corn with 14% and 28% defatted rice bran, respectively. Soybean meal, bran and soybean oil, etc. were used to balance the digestible energy, crude protein and amino acid levels among the basal diet and the two experimental diets. The adaption period was 10 days, and the experiment period was 28 days. The results showed as follows:1) the serum malondialdehyde (MDA) content of Suhuai pigs in the test group Ⅱ and test group Ⅲ were significantly lower than that in the test group Ⅰ (P<0.05); compared with the test group Ⅰ and test group Ⅲ, the serum catalase (CAT) activity of Suhuai pigs in the test group Ⅱ was significantly higher (P<0.05); Suhuai pigs in the test group Ⅲ had the highest activity of CuZn superoxide dismutase (CuZn-SOD) in serum, which was significantly different from that in the test group Ⅰ (P<0.05); the liver glutathione (GSH) content and the ratio of glutathione to oxidized glutathione (GSH/GSSG) of Suhuai pigs in the test group Ⅲ were significantly higher than those in the test group Ⅰ (P<0.05). 2) Compared with the test group Ⅰ, the serum contents of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) of Suhuai pigs in the test group Ⅲ were significantly higher (P<0.05); there was no significant difference in the contents of liver TC and triglyceride (TG) among all groups (P>0.05). 3) Diets with defatted rice bran partially replacement of corn had no significant effects on the mRNA expression levels of cholesterol-7alpha-hydroxylase 1 (CYP7α1) and cholesterol-27alpha-hydroxylase 1 (CYP27α1) in the liver of Suhuai pigs (P>0.05); the mRNA expression levels of 3-hydroxy-3-mothylglutaryl coenzyme A reductase (HMGCR), sterol regulatory element binding protein-2 (SREBP-2) and scaveger receptor class B type I (SR-BI) in the liver of Suhuai pigs in the test group Ⅱ and the test group Ⅲ were significantly lower than those in the test group Ⅰ (P<0.05). In addition, Suhuai pigs in the test group Ⅲ had the lowest low density lipoprotein receptor (LDLR) mRNA expression level in liver, which was significantly different from that in the test group Ⅰ (P<0.05). 4) Correlation analysis between serum contents of TC and LDL-C with oxidation marker and antioxidant enzymes in serum and liver of Suhuai pigs showed that serum TC content in the same individual was positively correlated with liver GSH content and GSH/GSSG (P<0.05). There was an extremely significant positive correlation between serum LDL-C content and liver GSH content as well as GSH/GSSG (P<0.01). In summary, under the conditions of this experiment, partial replacement of corn with different levels of defatted rice bran in the diet can improve the antioxidant capacity, and affect the cholesterol metabolism in serum and liver, and cholesterol-related gene expression in liver of Suhuai pigs.

Key words： defatted rice bran; Suhuai pigs; oxidative biomarker; antioxidant enzymes; cholesterol metabolism

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