哺乳期补饲精氨酸对断奶仔猪肝脏脂代谢功能的影响

刘军; 仲召鑫; 彭众; 喻礼怀; 董丽

doi:10.3969/j.issn.1006-267x.2020.02.023

动物营养学报 >

2020 , Vol. 32 >Issue 2: 674 - 681

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.023

猪营养与饲料 SWINE NUTRITION AND FEED

哺乳期补饲精氨酸对断奶仔猪肝脏脂代谢功能的影响

刘军 ,
仲召鑫 ,
彭众 ,
喻礼怀 ,
董丽

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扬州大学动物科学与技术学院, 扬州 225009

刘军(1998-),男,安徽池州人,本科生,研究方向为动物营养调控与饲料资源开发。E-mail:770772073@qq.com

收稿日期: 2019-08-20

网络出版日期: 2020-02-21

基金资助

江阴市科技创新专项（JY0603A010103180020PB）；江苏省自然科学基金（青年）项目（BK20170490）

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Effects of Arginine Supplementation during Lactation Period on Liver Lipid Metabolism Function of Weaned Piglets

LIU Jun ,
ZHONG Zhaoxin ,
PENG Zhong ,
YU Lihuai ,
DONG Li

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2019-08-20

Online published: 2020-02-21

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摘要

本文旨在研究哺乳期补饲精氨酸对断奶仔猪肝脏脂代谢功能的影响。试验成对选取7日龄体重相近40头哺乳仔猪，分为对照组（CON组）和精氨酸组（Arg组），每组20头猪（公母各占1/2）。7日龄起，在自然哺乳的同时，CON组仔猪每日灌服生理盐水40 mL，Arg组每日灌服精氨酸40 mL[250 mg/（kg·d]。在21日龄时，仔猪断奶并饲喂基础饲粮。每组在断奶当天（21日龄）和断奶后第3天（24日龄）每次随机挑选8头仔猪（公母各占1/2）屠宰并采取肝脏。结果表明：1）断奶后第3天，仔猪的体重与肝脏重均极显著低于断奶当天（P<0.01）；与CON组相比，断奶后第3天，Arg组仔猪肝脏指数极显著提高（P<0.01）。2）与CON组相比，Arg组仔猪肝细胞粒径极显著提高（P<0.01），肝细胞面积显著提高（P<0.05）。3）断奶后第3天，仔猪肝脏中总胆固醇（TC）、甘油三酯（TG）含量极显著高于断奶当天（P<0.01）；断奶当天，Arg组仔猪肝脏中高密度脂蛋白胆固醇（HDL-C）含量显著高于CON组（P<0.05），低密度脂蛋白胆固醇（LDL-C）含量极显著低于CON组（P<0.01）。4）与CON组相比，断奶当天，Arg组仔猪肝脏中脂蛋白脂肪酶（LPL）基因相对表达水平显著提高（P<0.05）。由此可得，哺乳期补饲精氨酸能够促进断奶仔猪肝脏发育，提高肝脏中HDL-C含量，降低肝脏中LDL-C含量，提高肝脏中LPL基因的表达水平，进而提高断奶仔猪肝脏脂代谢的功能。

关键词： 精氨酸; 仔猪; 肝脏; 脂代谢; 断奶应激

本文引用格式

刘军 , 仲召鑫 , 彭众 , 喻礼怀 , 董丽 . 哺乳期补饲精氨酸对断奶仔猪肝脏脂代谢功能的影响[J]. 动物营养学报, 2020 , 32(2) : 674 -681 . DOI: 10.3969/j.issn.1006-267x.2020.02.023

Abstract

The purpose of this study was to investigate the effects of arginine supplementation during the lactation period on liver lipid metabolism function of weaned piglets. Forty 7-day-old suckling piglets with similar body weight were selected in pairs and divided into control group (CON group) and arginine group (Arg group) with 20 heads in each group (half male and half female). From the 7 days of age, under the natural breastfeeding, piglets in the CON group were given 40 mL normal saline daily, and those in the Arg group were given 40 mL arginine[250 mg/(kg·d)] daily. All piglets were weaned at 21 days of age and then fed a basal diet. Every eight piglets (half male and half female) were randomly selected for slaughter sampling and taking liver on day of weaning (21 days of age) and 3 days after weaning (24 days of age), respectively. The results showed as follows:1) the body weight and liver weight of piglets after weaning for 3 days were extremely significantly lower than those of piglets at weaning day (P<0.01). Compared with the CON group, the liver index of piglets in the Arg group was extremely significantly increased after weaning for 3 days (P<0.01). 2) Compared with the CON group, the hepatocyte size was extremely significantly increased (P<0.01) and the hepatocyte area was significantly increased (P<0.05) of piglets in the Arg group. 3) Three days after weaning, the contents of liver total cholesterol (TC) and triglyceride (TG) of piglets were extremely significantly higher than those of piglets at the day of weaning (P<0.01); on the day of weaning, the high-density lipoprotein cholesterol (HDL-C) content in the liver of piglets in the Arg group was significantly higher than that in the CON group (P<0.05), and the low-density lipoprotein cholesterol (LDL-C) content in the liver of piglets in the Arg group was extremely significantly lower than in the CON group (P<0.01). 4) Compared with the CON group, the relative expression level of lipoprotein lipase (LPL) gene in the liver of piglets in the Arg group was significantly higher than that in the CON group on the day of weaning (P<0.05). It is concluded that arginine supplementation during the lactation period can promote liver development of weaned piglets, increase HDL-C content in liver, decrease LDL-C content in liver, increase the expression level of LPL gene in liver, and improve liver lipid metabolism function of weaned piglets.

Key words： arginine; piglets; liver; lipid metabolism; weaning stress

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