动物营养学报 >

2020 , Vol. 32 >Issue 2: 736 - 745

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.029

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

光周期变化对山羊褪黑素分泌及免疫和抗氧化功能的影响

  • 毛晨羽 ,
  • 邢媛媛 ,
  • 石璐璐 ,
  • 徐元庆 ,
  • 金晓 ,
  • 史彬林
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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018
毛晨羽(1992-),男,内蒙古包头人,博士研究生,从事动物环境与营养研究。E-mail:mcyyy1992@163.com

收稿日期: 2019-08-09

  网络出版日期: 2020-02-21

基金资助

国家重点研发计划项目（2016YFD0500508）

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Effects of Photoperiod Change on Melatonin Secretion, Immune and Antioxidant Function of Goats

  • MAO Chenyu ,
  • XING Yuanyuan ,
  • SHI Lulu ,
  • XU Yuanqing ,
  • JIN Xiao ,
  • SHI Binlin
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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2019-08-09

  Online published: 2020-02-21

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摘要

光周期可以影响动物体内褪黑素（MLT）的分泌，而MLT能参与机体多种生理功能的调节。本试验旨在研究光周期对山羊MLT分泌、免疫和抗氧化功能及相关基因表达的影响。将18只6月龄雌性绒山羊随机分为3组：对照（CG，自然光周期）组、短光照（SDPP，光照8 h/d，黑暗16 h/d）组和渐减光照（SIPP，光照时间从16 h/d逐渐缩短到8 h/d）组。试验期为60 d。结果表明：与对照组相比，1）在试验第30天，SDPP显著增加了血清MLT含量（P<0.05），在试验第60天，SIPP显著增加了血清MLT含量（P<0.05）；2）在试验第30天，SDPP组血清总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）活性显著升高（P<0.05），丙二醛（MDA）含量显著降低（P<0.05），而SIPP组上述指标均无显著变化（P>0.05）；在试验第60天，SDPP组和SIPP组血清T-SOD、GPx和CAT活性均显著升高（P<0.05），MDA含量显著降低（P<0.05）；3）在试验第30天，SDPP显著提高了血清免疫球蛋白G（IgG）、白细胞介素-1β（IL-1β）和白细胞介素-2（IL-2）含量（P<0.05）；在试验第60天，SDPP和SIPP均显著提高了血清IgG含量（P<0.05），此外，SDPP显著提高了血清IL-1β、IL-2和肿瘤坏死因子-α（TNF-α）含量（P<0.05），而SIPP仅显著提高了血清IL-1β含量（P<0.05）；4）在基因表达方面，在试验第30天，SDPP显著上调了白细胞中SOD1、GPx1、GPx4、CAT、核转录因子2（Nrf2）、IL-1β、和IL-2基因相对表达量（P<0.05）；在试验第60天，SDPP组白细胞中SOD1、CAT、GPx4、IL-1β和IL-2基因相对表达量显著提高（P<0.05），SIPP组白细胞中SOD1、GPx1、CAT、IL-1β和IL-2基因相对表达量显著提高（P<0.05）。上述结果表明，持续短光照能分泌更多的MLT，提高山羊的免疫和抗氧化功能。

关键词： 光周期变化; MLT分泌; 免疫功能; 抗氧化功能; 基因表达; 绒山羊

本文引用格式

毛晨羽 , 邢媛媛 , 石璐璐 , 徐元庆 , 金晓 , 史彬林 . 光周期变化对山羊褪黑素分泌及免疫和抗氧化功能的影响[J]. 动物营养学报, 2020 , 32(2) : 736 -745 . DOI: 10.3969/j.issn.1006-267x.2020.02.029

Abstract

Photoperiod can affect the secretion of melatonin (MLT), which can participate in a variety of physiological regulation. The purpose of this experiment was to study the effects of photoperiod on MLT secretion, immune and antioxidant function and related gene expression of goats. Eighteen 6-month-old female cashmere goats were randomly divided into 3 groups:control (CG, natural light) group; short-day photoperiod (SDPP, 8 h light,16 h dark) group and shortening-day photoperiod (SIPP, lighting time is gradually shortened from 16 to 8 h/day) group. The experimental period was 60 days. The results showed as follows:compared with control group, 1) SDPP significantly increased the content of MLT in goat serum (P<0.05) at day 30 of experiment, while SIPP significantly increased the content of MLT at day 60 of experiment (P<0.05). 2) The activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GPx) and catalase (CAT) in SDPP group significantly increased (P<0.05) and the content of malondialdehyde (MDA) significantly decreased (P<0.05) at day 30 of experiment. No effect of SIPP was observed (P>0.05). At the day 60 of experiment, the activities of serum T-SOD, GPx and CAT in SDPP group and SIPP group significantly increased (P<0.05), and the content of MDA significantly decreased (P<0.05). 3) At day 30 of experiment, the contents of immunoglobulin G (IgG), interleukin-1β (IL-1β) and interleukin-2 (IL-2) in serum significantly increased in SDPP group (P<0.05). At the day 60 of experiment, SDPP and SIPP both significantly increased the content of IgG in serum (P<0.05). For cytokines, SDPP significantly increased the contents of IL-1β, IL-2 and tumor necrosis factor-α (TNF-α) in serum (P<0.05), while SIPP only significantly increased the content of IL-1β in serum (P<0.05). 4) In terms of gene expression, SDPP significantly upregulated the gene relative expression levels of SOD1, GPx1, GPx4, CAT, nuclear transcription factor 2 (Nrf2), IL-1β, and IL-2 in leukocytes at day 30 of experiment (P<0.05). At day 60 of experiment, SDPP goat had higher gene relative expression levels of SOD1, CAT, GPx4, IL-1β and IL-2 in leukocytes (P<0.05). The gene relative expression levles of SOD1, GPx1, CAT, IL-1β and IL-2 in leukocytes significantly increased in SIPP (P<0.05). These results show that continuous short illumination can secrete more MLT, and improve goat's immune and antioxidant function.

Key words： photoperiod change; MLT secretion; immune function; antioxidant function; gene expression; goat

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