动物营养学报 >

2020 , Vol. 32 >Issue 2: 827 - 835

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.038

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

不同温度下添加乳酸菌对油莎草青贮品质及体外干物质消失率的影响

  • 李苗苗 ,
  • 靳思玉 ,
  • 王立超 ,
  • 周昕 ,
  • 黄秋连 ,
  • 王健 ,
  • 张嘉宾 ,
  • 曹阳
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  • 黑龙江八一农垦大学动物科技学院, 大庆 163319
李苗苗(1993-),女,甘肃白银人,硕士研究生,研究方向为饲料资源开发与利用。E-mail:mml_0303@163.com

收稿日期: 2019-07-26

  网络出版日期: 2020-02-21

基金资助

黑龙江八一农垦大学学成引进人才科研启动计划（XYB2013-11）；国家自然科学基金（31772673）

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Effects of Lactic Acid Bacteria Addition on Fermentation Quality and Dry Matter Digestibility of Cyperus esculentus Silage under Different Temperatures

  • LI Miaomiao ,
  • JIN Siyu ,
  • WANG Lichao ,
  • ZHOU Xin ,
  • HUANG Qiulian ,
  • WANG Jian ,
  • ZHANG Jiabin ,
  • CAO Yang
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  • College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2019-07-26

  Online published: 2020-02-21

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摘要

为研究不同乳酸菌添加剂及贮藏温度对油莎草青贮品质及体外干物质消失率的影响，试验采用双因素完全随机设计，将采集的油莎草原料分为3组，分别进行副干酪乳杆菌（AC组）和植物乳杆菌添加（LP组）及无添加处理（对照组），每组设10个重复。将以上处理的样品200 g装入食品级聚乙烯袋中进行真空密封，各取5袋分别置于常温（20±2）℃和低温4℃贮藏35 d。结果表明：1）常温处理下，LP组油莎草青贮有机物含量，AC组和LP组粗蛋白质、粗脂肪含量，乳酸菌数量及体外产气量，干物质消失率，丙酸含量均显著高于对照组（P<0.05）；AC组和LP组油莎草青贮pH，氨态氮/总氮，酵母菌、好氧菌数量及体外pH，乙酸含量均显著低于对照组（P<0.05）。2）低温处理下，AC组和LP组油莎草青贮乳酸含量，乳酸菌、芽孢杆菌数量，粗蛋白质含量及体外产气量，干物质消失率均显著高于对照组（P<0.05）；AC组和LP组油莎草青贮酵母菌、好氧菌数量及体外氨态氮含量均显著低于对照组（P<0.05）。3）乳酸菌添加和温度处理在油莎草青贮pH，乳酸菌、酵母菌、好氧菌及芽孢杆菌数量，有机物、粗蛋白质、粗脂肪含量，氨态氮/总氮及体外发酵产气量，干物质消失率，乳酸、乙酸和丙酸含量方面表现出极显著的交互作用（P<0.01）。本试验条件下，2种乳酸菌添加均能有效改善油莎草青贮品质，提高营养价值，添加植物乳杆菌可以改善低温环境下油莎草的青贮品质。

关键词： 油莎草; 乳酸菌; 发酵品质; 体外干物质消失率

本文引用格式

李苗苗 , 靳思玉 , 王立超 , 周昕 , 黄秋连 , 王健 , 张嘉宾 , 曹阳 . 不同温度下添加乳酸菌对油莎草青贮品质及体外干物质消失率的影响[J]. 动物营养学报, 2020 , 32(2) : 827 -835 . DOI: 10.3969/j.issn.1006-267x.2020.02.038

Abstract

In order to study the effects of different lactic acid bacteria additives and storage temperatures on the quality of Cyperus esculentus silage and the disappearance rate of dry matter in vitro, the experiment was designed completely randomly with two factors. Cyperus esculentus were divided into 3 group, and the addition treatments included the addition of Lactobacillus plantarum (AC group), Lactobacillus paracasei (LP group) and no addition (control group), and each group had 10 replicates; the treatment samples 200 g were putted into food grade polyethylene bag for vacuum sealing, and 5 bags samples were ensiled under normal temperature (20±2)℃ and low temperature 4℃ for 35 days, respectively. The results showed as follows:1) under normal temperature treatment, the organic matter content in LP group, crude protein and ether extract contents, lactic acid bacteria amount of Cyperus esculentus silage and in vitro gas production, dry matter disappearance rate and propionic acid content in AC and LP groups were significantly higher than those in the control group (P<0.05); pH, ammonia nitrogen/total nitrogen, yeast and aerobic bacteria amount of Cyperus esculentus silage and in vitro pH and acetic acid content in AC and LP groups were significantly lower than those in the control group (P<0.05). 2) Under low temperature treatment, the lactic acid content, lactic acid bacteria and Bacillus amount, organic matter and crude protein contents of Cyperus esculentus silage and in vitro gas production, dry matter disappearance rate in AC and LP groups were significantly higher than those in the control group (P<0.05); yeast and aerobic bacteria amount of Cyperus esculentus silage and ammonia nitrogen content in vitro were significantly lower than those in the control group (P<0.05). 3) Lactic acid bacteria additives and storage temperatures had significant interaction on pH, lactic acid bacteria, yeast, aerobic bacteria and Bacillus amount, organic matter, crude protein, ether extract contents, ammonia nitrogen/total nitrogen of Cyperus esculentus silage and in vitro fermentation gas production, dry matter disappearance rate, lactic acid, acetic acid and propionic acid contents (P<0.01). The two kind of lactic acid bacteria can improve the fermentation quality and nutritional value of the Cyperus esculentus silage under the conditions of this test. Lactobacillus plantarum can improve the fermentation quality of the Cyperus esculentus silage under low temperature.

Key words： Cyperus esculentus; lactic acid bacteria; fermentation quality; dry matter disappearance rate in vitro

参考文献

[1] 王瑞元,王晓松,相海.一种多用途的新兴油料作物——油莎豆[J].中国油脂,2019,44(1):1-4.
[2] 阳振乐.油莎豆的特性及其研究进展[J].北方园艺,2017(17):192-201.
[3] 张学昆.我国油莎豆产业研发进展报告[J].中国农村科技,2019(4):67-69.
[4] 刘建新,杨振海,叶均安,等.青贮饲料的合理调制与质量评定标准[J].饲料工业,1999,20(4):3-5.
[5] CAO Y M,CAI Y M,HIRAKUBO T,et al.Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons[J].Animal Science Journal,2011,82(2):259-266.  
[6] CAI Y,BENNO Y,OGAWA M,et al.Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage[J].Journal of Dairy Science,1999,82(3):520-526.  
[7] AOAC.Official methods of analysis[M].15th ed.Artington:Association of Official Analytical Chemists,1990.
[8] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber, neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.  
[9] MCDOUGALL E I.Studies on ruminant saliva.1.The composition and output of sheep's saliva[J].Biochemical Journal,1948,43(1):99-109.
[10] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993(4):40-41.
[11] 贾鹏禹,孙蕊,李井春,等.离子排斥色谱法测定瘤胃液中乳酸和挥发性脂肪酸的含量[J].黑龙江畜牧兽医,2017(3):43-46.
[12] 张红梅,段珍,李霞,等.青贮饲料乳酸菌添加剂的应用现状[J].草业科学,2017,34(12):2575-2583.
[13] KUNG L Jr,SHAVER R D,GRANT R J,et al.Silage review:interpretation of chemical,microbial,and organoleptic components of silages[J].Journal of Dairy Science,2018,101(5):4020-4033.  
[14] MUCK R.Recent advances in silage microbiology[J].Agricultural and Food Science,2013,22(1):3-15.  
[15] 张相伦,游伟,赵红波,等.乳酸菌制剂对全株玉米青贮品质及营养成分的影响[J].动物营养学报,2018,30(1):336-342.
[16] 万学瑞,吴建平,雷赵民,等.优良抑菌活性乳酸菌对玉米青贮及有氧暴露期微生物数量和pH的影响[J].草业学报,2016,25(4):204-211.
[17] MUCH R E,NADEAU E M G,MCALLISTER T A,et al.Silage review:recent advances and future uses of silage additives[J].Journal of Dairy Science,2018,101(5):3980-4000.  
[18] 牛化欣,常杰,胡宗福,等.青贮微生物的发掘及其应用的研究进展[J].动物营养学报,2018,30(11):4279-4285.
[19] 贾婷婷,吴哲,玉柱.不同类型乳酸菌添加剂对燕麦青贮品质和有氧稳定性的影响[J].草业科学,2018,35(5):1266-1272.
[20] 姜伯玲,王曙阳,陈积红,等.发酵温度对复合微生物菌剂青贮饲料品质的影响[J].中国草食动物科学,2016,36(2):29-32.
[21] HASHEMZADEH-CIGARI F,KHORVASH M,GHORBANI G R,et al.Interactive effects of molasses by homofermentative and heterofermentative inoculants on fermentation quality, nitrogen fractionation,nutritive value and aerobic stability of wilted alfalfa (Medicago sativa L.) silage[J].Journal of Animal Physiology and Animal Nutrition,2014,98(2):290-299.  
[22] SILVA V P,PEREIRA O G,LEANDRO E S,et al.Effects of lactic acid bacteria with bacteriocinogenic potential on the fermentation profile and chemical composition of alfalfa silage in tropical conditions[J].Journal of Dairy Science,2016,99(3):1895-1902.  
[23] 董志浩,原现军,闻爱友,等.添加乳酸菌和发酵底物对桑叶青贮发酵品质的影响[J].草业学报,2016,25(6):167-174.
[24] 桂荣,刘晗璐.披碱草和老芒麦附着微生物检测及添加乳酸菌对其青贮发酵品质的影响[J].中国饲料,2007(13):39-42.
[25] 于满满,姜雨轩,张美美,等.利用体外产气法评定不同比例氨化秸秆替代苜蓿的组合效应[J].中国畜牧兽医,2017,44(12):3497-3504.
[26] NSAHLAI I V,SIAW D,OSUJI P O.The relationships between gas production and chemical composition of 23 browses of the genus Sesbania[J].Journal of the science of food and agriculture,1994,65(1):13-20.  
[27] 玉柱,魏馨,于艳冬,等.添加剂对尖叶胡枝子青贮发酵品质及体外消化率的影响[J].草业学报,2009,18(5):73-79.
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